Jeffrey M. Linnen - Poway CA, US Wen Wu - Carlsbad CA, US
Assignee:
Gen-Probe Incorporated - San Diego CA
International Classification:
C12Q 1/68
US Classification:
435 6
Abstract:
Compositions, methods and kits for detecting the nucleic acids of HIV-1, HIV-2, or the combination of HIV-1 and HIV-2. Particularly described are oligonucleotides that are useful as hybridization probes and amplification primers, including cross-reacting hybridization probes and cross-reacting amplification primers, for detecting very low levels of viral nucleic acids.
Sample Preparation Method Incorporating An Alkaline Shock
Kui Gao - San Diego CA, US Michael M. Becker - San Diego CA, US Wen Wu - Carlsbad CA, US Jeffrey M. Linnen - Poway CA, US
Assignee:
Gen-Probe Incorporated - San Diego CA
International Classification:
C12Q 1/68
US Classification:
435 6
Abstract:
Method of preparing a biological sample appropriate for use in a subsequent in vitro nucleic acid amplification reaction. The method involves a rapid, transient exposure to alkaline conditions which can be achieved by mixing an alkaline solution with a pH-buffered solution that includes a detergent and the biological sample to be tested for the presence of particular nucleic acid species using in vitro amplification. The invented method advantageously can improve detection of some target nucleic acids without substantially compromising detectability of others. The method is particularly useful for simultaneously preparing RNA and DNA templates that can be used in multiplex amplification reactions.
Cross-Reactive Primers For Amplifying The Nucleic Acids Of Hiv-1 And Hiv-2
Compositions, methods and kits for detecting the nucleic acids of HIV-1, HIV-2, or the combination of HIV-1 and HIV-2. Particularly described are oligonucleotides that are useful as hybridization probes and amplification primers, including cross-reacting hybridization probes and cross-reacting amplification primers, for detecting very low levels of viral nucleic acids.
Method For Detecting West Nile Virus Nucleic Acids In The 5′ Non-Coding/Capsid Region
Jeffrey M. Linnen - Poway CA, US Reinhold P. Pollner - San Diego CA, US Wen Wu - Carlsbad CA, US Geoffrey G. Dennis - San Diego CA, US
Assignee:
Gen-Probe Incorporated - San Diego CA
International Classification:
C12Q 1/68 C12P 19/32
US Classification:
435 912, 435 6, 435 911
Abstract:
Methods for detecting flavivirus nucleic acids. Particularly described are methods for detecting very low levels of West Nile virus nucleic acids in the 5′ non-coding/capsid region.
Kui Gao - San Diego CA, US Michael M. Becker - San Diego CA, US Wen Wu - Carlsbad CA, US Jeffrey M. Linnen - Poway CA, US
Assignee:
Gen-Probe Incorporated - San Diego CA
International Classification:
C12P 19/34 C07H 1/08
US Classification:
435 912, 536 254
Abstract:
Method of preparing a biological sample appropriate for use in a subsequent in vitro nucleic acid amplification reaction. The method involves a rapid, transient exposure to alkaline conditions which can be achieved by mixing an alkaline solution with a pH-buffered solution that includes a detergent and the biological sample to be tested for the presence of particular nucleic acid species using in vitro amplification. The invented method advantageously can improve detection of some target nucleic acids without substantially compromising detectability of others. The method is particularly useful for simultaneously preparing RNA and DNA templates that can be used in multiplex amplification reactions.
Method Of Isolating Nucleic Acids From A Biological Sample
Kui Gao - San Diego CA, US Michael M. Becker - San Diego CA, US Wen Wu - Carlsbad CA, US Jeffrey M. Linnen - Poway CA, US
Assignee:
Gen-Probe Incorporated - San Diego CA
International Classification:
C12Q 1/68
US Classification:
435 61
Abstract:
Method of preparing a biological sample appropriate for use in a subsequent in vitro nucleic acid amplification reaction. The method involves a rapid, transient exposure to alkaline conditions which can be achieved by mixing an alkaline solution with a pH-buffered solution that includes a detergent and the biological sample to be tested for the presence of particular nucleic acid species using in vitro amplification. The invented method advantageously can improve detection of some target nucleic acids without substantially compromising detectability of others. The method is particularly useful for simultaneously preparing RNA and DNA templates that can be used in multiplex amplification reactions.
Cross-Reactive Hybridization Probe For Detecting Hiv-1 And Hiv-2 Nucleic Acids In The P31 Gene Sequence
Cross-reacting hybridization probe for detecting HIV-1 and HIV-2 nucleic acids. The probe advantageously exhibited uniformly high signal-to-noise ratios when hybridized to HIV-1 and HIV-2 target nucleic acids. The probe can be used, for example, in screening applications for detecting donated blood contaminated with either of the two analytes.
Alkaline Shock-Based Method Of Processing A Biological Sample
Kui Gao - San Diego CA, US Michael M. Becker - San Diego CA, US Wen Wu - Carlsbad CA, US Jeffrey M. Linnen - Poway CA, US
Assignee:
Gen-Probe Incorporated - San Diego CA
International Classification:
C12Q 1/68 C12P 19/34
US Classification:
435 61, 435 912
Abstract:
Method of processing a biological sample to yield nucleic acid appropriate for use in a subsequent in vitro nucleic acid amplification reaction. The method involves a rapid, transient exposure to alkaline conditions which can be achieved by mixing an alkaline solution with a pH-buffered solution that includes a detergent and the biological sample to be tested for the presence of particular nucleic acid species using in vitro amplification. The invented method advantageously can improve detection of some target nucleic acids without substantially compromising detectability of others. The method is particularly useful for simultaneously preparing RNA and DNA templates that can be used in multiplex amplification reactions.