Wenting Zhang

US Patent:

20130143230, Jun 6, 2013

Filed:

Nov 30, 2012

Appl. No.:

13/690831

Inventors:

The Trustees of the Stevens Institute of Technolog - Hoboken NJ, US
Woo Young Lee - Ridgewood NJ, US
Arthur B. Ritter - Morristown NJ, US
Xiaojun Yu - Fishers IN, US
Hongjun Wang - Millburn NJ, US
Henry Du - Short Hills NJ, US
Chang-Hwan Choi - Palisades Park NJ, US
Wenting Zhang - Jersey City NJ, US
Yexin Gu - North Bergen NJ, US

Assignee:

The Trustees of the Stevens Institute of Technology - Hoboken NJ

International Classification:

C12Q 1/02

US Classification:

435 71, 4352891, 4352972, 4352887, 4352872, 435 34

Abstract:

A microfluidic-based platform with cultured three-dimensional tissues simulates major human physiological systems for rapid evaluation of individual drugs prior to clinical testing or for personalized medical applications. The platform integrates the circulatory and lymphatic systems in a physiologically correct manner. The physiological systems may be simulated in the platform by microfluidic tissue culture devices which accommodate various tissues and provide integrated microvascular and lymphatic systems. Biomimetic nanofiber meshes or microfiber structures may be used to provide the cells with a physiologically relevant substrate. Each device may have an on-board detection system utilizing optical fiber bundles for microarray multiplexing of biomarkers, label-free SERS measurement of drugs, and microendoscopic confocal imaging of cells and tissues.

US Patent:

20160137986, May 19, 2016

Filed:

Dec 17, 2015

Appl. No.:

14/973329

Inventors:

- Hackensack NJ, US
- Hoboken NJ, US
David Samuel Siegel - West Orange NJ, US
Peter Tolias - Westfield NJ, US
Hongjun Wang - Milburn NJ, US
Wenting Zhang - Jersey City NJ, US

International Classification:

C12N 5/09

Abstract:

The described invention provides an ex vivo dynamic multiple myeloma (MM) cancer niche contained in a microfluidic device. The dynamic MM cancer niche includes (a) a three-dimensional tissue construct containing a dynamic ex vivo bone marrow (BM) niche, which contains a mineralized bone-like tissue containing viable osteoblasts self-organized into cohesive multiple cell layers and an extracellular matrix secreted by the viable adherent osteoblasts; and a microenvironment dynamically perfused by nutrients and dissolved gas molecules; and (b) human myeloma cells seeded from a biospecimen composition comprising mononuclear cells and the multiple myeloma cells. The human myeloma cells are in contact with osteoblasts of the BM niche, and the viability of the human myeloma cells is maintained by the MM cancer niche.

US Patent:

20150086993, Mar 26, 2015

Filed:

Sep 19, 2014

Appl. No.:

14/490847

Inventors:

- Hoboken NJ, US
- Hackensack NJ, US
Qiaoling Sun - Secaucus NJ, US
Wenting Zhang - Kearny NJ, US
Jenny Zilberberg - Yardley PA, US

Assignee:

THE TRUSTEES OF THE STEVENS INSTITUTE OF TECHNOLOGY - Hoboken NJ

International Classification:

C12N 5/077
C12M 3/06
C12M 1/12
G01N 33/50

US Classification:

435 612, 435377, 435 29, 4352971, 435 613

Abstract:

A bed of microbeads is used as a foundation for reconstructing a three-dimensional osteocyte network by culturing osteocytes within the bed. The osteocytes are cultured such that they form a network among the microbeads that is capable of simulating the osteocyte network of natural bone. The osteocytes are cultured in a microfluidic device adapted for the purpose.

US Patent:

20140274953, Sep 18, 2014

Filed:

Mar 14, 2013

Appl. No.:

13/827170

Inventors:

Woo Lee - Ridgewood NJ, US
Jenny Zilberberg - Yardley PA, US
David Samuel Siegel - West Orange NJ, US
Peter Tolias - Westfield NJ, US
Hongjun Wang - Milburn NJ, US
Wenting Zhang - Jersey City NJ, US

International Classification:

G01N 33/50

US Classification:

514 64, 435 723, 435366, 514 94, 514108, 514323

Abstract:

The described invention provides an ex vivo dynamic multiple myeloma (MM) cancer niche contained in a microfluidic device. The dynamic MM cancer niche includes (a) a three-dimensional tissue construct containing a dynamic ex vivo bone marrow (BM) niche, which contains a mineralized bone-like tissue containing viable osteoblasts self-organized into cohesive multiple cell layers and an extracellular matrix secreted by the viable adherent osteoblasts; and a microenvironment dynamically perfused by nutrients and dissolved gas molecules; and (b) human myeloma cells seeded from a biospecimen composition comprising mononuclear cells and the multiple myeloma cells. The human myeloma cells are in contact with osteoblasts of the BM niche, and the viability of the human myeloma cells is maintained by the MM cancer niche.