William J Kopaciewicz

US Patent:

6635201, Oct 21, 2003

Filed:

Apr 14, 1999

Appl. No.:

09/291355

Inventors:

William Kopaciewicz - West Newbury MA
Donald G. Sheer - Norfolk MA
Todd E. Arnold - Beverly MA
Vinay Goel - Acton MA

Assignee:

Millipore Corporation - Bedford MA

International Classification:

B29C 6500

US Classification:

264 41, 2105021, 21050027, 21050041, 21032175, 422100

Abstract:

A method for casting-in-place composite and/or non-filled structures which are useful as sorptive or reactive media or for size-based separations. Any particular housing size or configuration can be used, and the inclusion of a large amount of adsorptive particles in polymer is achieved while still maintaining the membrane three dimensional structure. In a first preferred embodiment, the composite structures comprise particles entrapped within a porous polymeric substrate, and are cast in-place into a housing such as a pipette tip, thereby providing an effective platform for micromass handling. With the appropriate selection of particle chemistry, virtually any separation or purification operation can be conducted, including selective bind/elute chromatography operations, on sample mass loads less than 1 microgram in volumes of a few microliters, as well as larger mass loads and volumes. The present invention also encompasses the composite structures as well as sample preparation devices containing the same. In a second structures are cast in situ in a suitable housing and can be used for size-based separations wherein the cast structure acts as a semi-permeable barrier.

US Patent:

6830717, Dec 14, 2004

Filed:

Jul 31, 2002

Appl. No.:

10/209390

Inventors:

William Kopaciewicz - West Newbury MA
Donald G. Sheer - Norfolk MA
Todd E. Arnold - Beverly MA
Vinay Goel - Acton MA

Assignee:

Millipore Corporation - Bedford MA

International Classification:

B29C 6500

US Classification:

264 41, 2105021, 21050041, 21050036, 21050042, 21032175, 422101

Abstract:

A method for casting-in-place composite and/or non-filled structures which are useful as sorptive or reactive media or for size-based separations. Any particular housing size or configuration can be used, and the inclusion of a large amount of adsorptive particles in polymer is achieved while still maintaining the membrane three dimensional structure. In a first preferred embodiment, the composite structures comprise particles entrapped within a porous polymeric substrate, and are cast in-place into a housing such as a pipette tip, thereby providing an effective platform for micromass handling. With the appropriate selection of particle chemistry, virtually any separation or purification operation can be conducted, including selective bind/elute chromatography operations, on sample mass loads less than 1 microgram in volumes of a few microliters, as well as larger mass loads and volumes. The present invention also encompasses the composite structures as well as sample preparation devices containing the same. In a second preferred embodiment, self-retaining, self-supporting structures are cast in situ in a suitable housing and can be used for size-based separations wherein the cast structure acts as a semi-permeable barrier.

US Patent:

6869572, Mar 22, 2005

Filed:

Sep 11, 2000

Appl. No.:

09/659241

Inventors:

William Kopaciewicz - West Newbury MA, US
Cheryl Brucato - Andover MA, US

Assignee:

Millipore Corporation - Bedford MA

International Classification:

B01D029/05

US Classification:

422101, 2103231, 210348, 210406, 210483, 422 69, 4352884, 4352975, 4353052

Abstract:

A card or insert having a plurality of recesses for a sample preparation device, the card containing cast-in-place composite and/or non-filled structures which are useful as sorptive or reactive media or for size-based separations. Any particular card size or configuration can be used, and the inclusion of a large amount of adsorptive particles in polymer is achieved while still maintaining the membrane three dimensional structure. In a first preferred embodiment, the composite structures comprise particles entrapped within a porous polymeric substrate, and are cast in-place into a plurality of recesses in an insert for a multi-well sample preparation device, thereby providing an effective platform for high throughput micromass handling. With the appropriate selection of particle chemistry, virtually any separation or purification operation can be conducted in multiplicity, including selective bind/elute chromatography operations, on sample mass loads less than 1 microgram in volumes of a few microliters, as well as larger mass loads and volumes. Manufacturing flexibility and high throughput is achieved.

US Patent:

6875354, Apr 5, 2005

Filed:

Sep 1, 1999

Appl. No.:

09/387443

Inventors:

William Kopaciewicz - West Newbury MA, US

Assignee:

Millipore Corporation - Billerica MA

International Classification:

B01D063/00
G01N015/06

US Classification:

21032175, 2103216, 21032184, 21050041, 21050035, 2105021, 21050042, 422 681, 422100, 422283

Abstract:

A method for casting-in-place composite and/or non-filled structures which are useful as sorptive or reactive media or for size-based separations. Any particular housing size or configuration can be used, and the inclusion of a large amount of adsorptive particles in polymer is achieved while still maintaining the membrane three dimensional structure. In a first preferred embodiment, the composite structures comprise particles entrapped within a porous polymeric substrate, and are cast in-place into a housing such as a pipette tip, thereby providing an effective platform for micromass handling. With the appropriate selection of particle chemistry, virtually any separation or purification operation can be conducted, including selective bind/elute chromatography operations, on sample mass loads less than 1 microgram in volumes of a few microliters, as well as larger mass loads and volumes. The present invention also encompasses the composite structures as well as sample preparation devices containing the same. In a second preferred embodiment, self-retaining, self-supporting structures are cast in situ in a suitable housing and can be used for size-based separations wherein the cast structure acts as a semi-permeable barrier.

US Patent:

6998047, Feb 14, 2006

Filed:

Oct 27, 2000

Appl. No.:

09/698378

Inventors:

William Kopaciewicz - West Newbury MA, US
Donald G. Sheer - Norfolk MA, US
Todd E. Arnold - Beverly MA, US
Vinay Goel - Acton MA, US

Assignee:

Millipore Corporation - Billerica MA

International Classification:

B01D 63/00

US Classification:

21032175, 2105021, 422101

Abstract:

A method for casting-in-place composite and/or non-filled structures which are useful as sorptive or reactive media or for size-based separations. Any particular housing size or configuration can be used, and the inclusion of a large amount of adsorptive particles in polymer is achieved while still maintaining the membrane three dimensional structure. In a first preferred embodiment, the composite structures comprise particles entrapped within a porous polymeric substrate, and are cast in-place into a housing such as a pipette tip, thereby providing an effective platform for micromass handling. With the appropriate selection of particle chemistry, virtually any separation or purification operation can be conducted, including selective bind/elute chromatography operations, on sample mass loads less than 1 microgram in volumes of a few microliters, as well as larger mass loads and volumes. The present invention also encompasses the composite structures as well as sample preparation devices containing the same. In a second preferred embodiment, self-retaining, self-supporting structures are cast in situ in a suitable housing and can be used for size-based separations wherein the cast structure acts as a semi-permeable barrier.

US Patent:

7309458, Dec 18, 2007

Filed:

Oct 20, 2003

Appl. No.:

10/689121

Inventors:

William Kopaciewicz - West Newbury MA, US
Phillip Clark - Wakefield MA, US
David Brewster - Beverly MA, US

Assignee:

Millipore Corporation - Billerica MA

International Classification:

B29C 65/00

US Classification:

264 41, 422101, 422102, 2103216, 21050022, 21050027

Abstract:

Sample preparation device and method for desalting and concentrating samples prior to further analysis such as by MALDI TOF and/or electro-spray ionization (ESI) mass spectrometry. The device in accordance with an embodiment of the present invention contains a three dimensional structure preferably comprising a plurality of sorptive particles entrapped in a porous polymer matrix so as to form a device capable of carrying out solid phase extraction. The device is manufactured by introducing casting solution containing polymer and optionally particles into a housing, and subsequently exposing the device to a quench bath for a time sufficient to allow for solvent exchange and precipitation to form the composite structure in the housing. The present invention is also directed towards a method of sample preparation using the device of the present invention.

US Patent:

7695686, Apr 13, 2010

Filed:

Dec 9, 2004

Appl. No.:

11/008062

Inventors:

William Kopaciewicz - West Newbury MA, US
Phillip Clark - Wakefield MA, US
David Brewster - Beverly MA, US

Assignee:

Millipore Corporation - Billerica MA

International Classification:

B01L 11/00

US Classification:

422101, 422100, 422102, 436177, 436178

Abstract:

Sample preparation device and method for desalting and concentrating samples prior to further analysis such as by MALDI TOF and/or electro-spray ionization (ESI) mass spectrometry. The device in accordance with an embodiment of the present invention contains a three dimensional structure preferably comprising a plurality of sorptive particles entrapped in a porous polymer matrix so as to form a device capable of carrying out solid phase extraction. The device is manufactured by introducing casting solution containing polymer and optionally particles into a housing, and subsequently exposing the device to a quench bath for a time sufficient to allow for solvent exchange and precipitation to form the composite structure in the housing. The present invention is also directed towards a method of sample preparation using the device of the present invention.

US Patent:

7951611, May 31, 2011

Filed:

Jul 22, 2004

Appl. No.:

10/897169

Inventors:

William Kopaciewicz - West Newbury MA, US
Cheryl Brucato - Andover MA, US

Assignee:

Millipore Corporation - Billerica MA

International Classification:

B01D 15/10

US Classification:

436183, 436177, 436178, 422534, 422535

Abstract:

A card or insert having a plurality of recesses for a sample preparation device, the card containing cast-in-place composite and/or non-filled structures which are useful as sorptive or reactive media or for size-based separations. Any particular card size or configuration can be used, and the inclusion of a large amount of adsorptive particles in polymer is achieved while still maintaining the membrane three dimensional structure. In a first preferred embodiment, the composite structures comprise particles entrapped within a porous polymeric substrate, and are cast in-place into a plurality of recesses in an insert for a multi-well sample preparation device, thereby providing an effective platform for high throughput micromass handling. With the appropriate selection of particle chemistry, virtually any separation or purification operation can be conducted in multiplicity, including selective bind/elute chromatography operations, on sample mass loads less than 1 microgram in volumes of a few microliters, as well as larger mass loads and volumes. Manufacturing flexibility and high throughput is achieved.