William R Light

US Patent:

6518010, Feb 11, 2003

Filed:

Feb 28, 2001

Appl. No.:

09/795821

Inventors:

Maria S. Gawryl - Charlestown MA
Robert A. Houtchens - Milford MA
William R. Light - Natick MA

Assignee:

Biopure Corporation - Cambridge MA

International Classification:

A01N 102

US Classification:

435 2, 514 6, 530380, 530385

Abstract:

Red blood cells are purified by defibrinating whole blood and then filtering the defibrinated whole blood, whereby at least a portion of a plasma component is separated from the red blood cells to form a suspension of red blood cells, thereby purifying the red blood cells. Whole blood is defibrinated by, for example, using a chemical coagulating agent or mechanical agitation. Separation of the plasma component from red blood cells can be completed by, for example, diafiltration. The suspension of red blood cells can then be employed to produce a hemoglobin-based oxygen carrier.

US Patent:

6610832, Aug 26, 2003

Filed:

Jul 7, 1999

Appl. No.:

09/349290

Inventors:

Maria S. Gawryl - Charlestown MA
Robert A. Houtchens - Milford MA
William R. Light - Natick MA

Assignee:

Biopure Corporation - Cambridge MA

International Classification:

C07K 14805

US Classification:

530385, 514 6, 604403, 604408

Abstract:

The invention relates to a method for preserving the stability of a hemoglobin blood substitute comprising maintaining the hemoglobin blood substitute in an atmosphere substantially free of oxygen. The invention also involves a method for producing a stable polymerized hemoglobin blood-substitute from blood. The method of this invention includes mixing blood with an anticoagulant to form a blood solution, washing the red blood cells in the blood solution and then separating the washed red blood cells from the white blood cells. This method also includes disrupting the red blood cells to release hemoglobin and form a hemoglobin solution, which is then treated by high performance liquid chromatography to form a hemoglobin eluate. The hemoglobin eluate is then deoxygenated, contacted with a first sulfhydryl compound to form an oxidation-stabilized deoxygenated hemoglobin solution, and mixed with a cross-linking agent to form a polymerization reaction mixture, which is then polymerized. The polymerized hemoglobin solution is then diafiltered with a physiologic solution and with a sulfhydryl compound, whereby the polymerized hemoglobin solution is made physiologically acceptable, and whereby the sulfhydryl compound scavenges oxygen, to form a stable polymerized hemoglobin blood-substitute, which is then packaged and stored in an atmosphere substantially free of oxygen.

US Patent:

6811778, Nov 2, 2004

Filed:

Sep 10, 2001

Appl. No.:

09/950270

Inventors:

Thomas C. Page - Watertown MA
William R. Light - Natick MA

Assignee:

Biopure Corporation - Cambridge MA

International Classification:

A01N 6300

US Classification:

424 9373, 424533, 514 6

Abstract:

A method of treatment of a patient includes administering to the patient stored red blood cells and a hemoglobin solution. The stored red blood cells and the hemoglobin solution can be administered to the patient simultaneously. Alternatively, the hemoglobin solution can be administered to the patient prior to administrating the stored red blood cells or the stored red blood cells can be administered to the patient prior to administering the hemoglobin solution. A composition of the invention includes stored red blood cells and a hemoglobin solution.

US Patent:

6986984, Jan 17, 2006

Filed:

Nov 26, 2002

Appl. No.:

10/306819

Inventors:

Maria S. Gawryl - Charlestown MA, US
Robert A. Houtchens - Milford MA, US
William R. Light - Natick MA, US

Assignee:

Biopure Corporation - Cambridge MA

International Classification:

A01N 1/02
A61K 38/17
C07K 1/00

US Classification:

435 2, 424529, 530354, 530363, 530380, 530385, 530829

Abstract:

Red blood cells are purified by defibrinating whole blood and then filtering the defibrinated whole blood, whereby at least a portion of a plasma component is separated from the red blood cells to form a suspension of red blood cells, thereby purifying the red blood cells. Whole blood is defibrinated by, for example, using a chemical coagulating agent or mechanical agitation. Separation of the plasma component from red blood cells can be completed by, for example, diafiltration. The suspension of red blood cells can then be employed to produce a hemoglobin-based oxygen carrier.

US Patent:

7001715, Feb 21, 2006

Filed:

Feb 28, 2002

Appl. No.:

10/090428

Inventors:

Robert A. Houtchens - Milford MA, US
Maria S. Gawryl - East Boston MA, US
William R. Light - Natick MA, US
Javed Baqai - Lexington MA, US

Assignee:

Biopure Corporation - Cambridge MA

International Classification:

A01N 1/02
A61K 38/16
A61K 35/14
C07K 17/00

US Classification:

435 2, 424529, 424533, 514 6, 530380, 530385

Abstract:

Red blood cells are purified by separating whole blood, such as by centrifugation, to form a red blood cell fraction and a liquid fraction. The whole blood can be defibrinated or treated to prevent coagulation prior to separation. Preferably, the whole blood is bovine blood. The red blood cell fraction is then diafiltered to purify the red blood cells. The purified red blood cells can then be lysed to form a lysate of purified red blood cells. The purified red blood cells and the lysate of purified red blood cells are suitable for use in producing hemoglobin blood substitute.

US Patent:

7041799, May 9, 2006

Filed:

Jul 7, 2000

Appl. No.:

10/018529

Inventors:

Maria S. Gawryl - East Boston MA, US
Robert A. Houtchens - Milford MA, US
William R. Light - Natick MA, US

Assignee:

Biopure Corporation - Cambridge MA

International Classification:

C07K 14/805
A61B 19/02

US Classification:

530385, 514 6, 604403, 604408

Abstract:

The invention relates to a method for preserving the stability of a hemoglobin blood substitute comprising maintaining the hemoglobin blood substitute in an atmosphere substantially free of oxygen. The method for preserving the deoxygenated hemoglobin blood substitute comprises maintaining the deoxygenated blood substitute in an oxygen barrier film overwrap package, wherein at least one face of the overwrap package comprises a transparent laminate material and wherein at least one other face of the overwrap package comprises a foil laminate material. The preserved deoxygenated hemoglobin blood substitute comprises a deoxygenated hemoglobin blood substitute and an oxygen barrier film overwrap package wherein at least one face of the overwrap package comprises a transparent laminate material and wherein at least one other face of the overwrap package comprises a foil laminate material.

US Patent:

7041800, May 9, 2006

Filed:

Jul 7, 2000

Appl. No.:

10/018599

Inventors:

Maria S. Gawryl - East Boston MA, US
Robert A. Houtchens - Milford MA, US
William R. Light - Natick MA, US

Assignee:

Biopure Corporation - Cambridge MA

International Classification:

C07K 14/805
A61B 19/02

US Classification:

530385, 514 6, 604403, 604408

Abstract:

The invention relates to a method for preserving the stability of a hemoglobin blood substitute comprising maintaining the hemoglobin blood substitute in an atmosphere substantially free of oxygen. The method for preserving a deoxygenated hemoglobin blood substitute comprises maintaining the deoxygenated hemoglobin blood substitute in an oxygen barrier film overwrap package. In one embodiment, the package comprises a transparent laminate material comprising an oxygen barrier layer and a polyolefin layer, wherein the laminant has a thickness of between about 0. 001 and about 0. 01 inches (or about 0. 0254 to about 0. 254 millimeters) and an oxygen permeability of less than about 0. 01 cubic centimeters per 100 square inches (or about 0. 01 cc per 645 square centimeters) over 24 hours at 1 atmosphere and at about 23 C. The oxygen barrier layer comprises ethylene vinyl alcohol.

US Patent:

7135554, Nov 14, 2006

Filed:

Nov 29, 2004

Appl. No.:

10/998861

Inventors:

Thomas C. Page - Watertown MA, US
Jose O. Torres - Norton MA, US
William R. Light - Natick MA, US

Assignee:

Biopure Corporation - Cambridge MA

International Classification:

C07K 14/805

US Classification:

530385, 530402, 514 6

Abstract:

A stabilized hemoglobin solution is contacted with polymerizing agent. The stabilized hemoglobin solution includes stabilized tetrameric hemoglobin. At least a portion of the stabilized tetrameric hemoglobin is polymerized by reaction with the polymerizing agent, thereby producing a polymerized hemoglobin solution. In one embodiment, the stabilized hemoglobin solution includes a filtrate formed by filtrating polymerized solution of native hemoglobin through a filter having a molecular weight cut off of about 100 kD.