William R Majarian

US Patent:

56838689, Nov 4, 1997

Filed:

Oct 6, 1994

Appl. No.:

8/244376

Inventors:

Robert Alan LaRossa - West Chester PA
William Robert Majarian - Mount Royal NJ
Tina Kangas Van Dyk - Wilmington DE

Assignee:

E. I. Du Pont de Nemours and Company - Wilmington DE

International Classification:

C12Q 168
C12N 121
C07H 2104

US Classification:

435 6

Abstract:

Subtle changes in environmental stress can now be detected and measured at sublethal levels as a generic response to environmental stress. The present invention provides a method to detect changes in the environmental stress level. The stress change is indicated as a change in the luminescence output of a genetically engineered microorganism. In the present invention, the luminescence gene complex is under the control of a stress inducible promoter.

US Patent:

51127490, May 12, 1992

Filed:

Oct 2, 1987

Appl. No.:

7/104735

Inventors:

Robert N. Brey - Rochester NY
William R. Majarian - Pittsford NY
Subramonia Pillai - Rochester NY
Wayne T. Hockmeyer - Pittsford NY

Assignee:

Praxis Biologics, Inc. - Rochester NY

International Classification:

C12N 1500
C12N 121
C12N 100
C12P 2102
A01N 6300
C07H 1512
C07K 300

US Classification:

4351723

Abstract:

The present invention is directed to attenuated strains of enteroinvasive bacteria that express a peptide or protein related to an epitope of the malaria parasites of the genus Plasmodium. The bacterial strains of the invention which can multiply in a host without causing significant disease or disorder, and which express a Plasmodium-related peptide that induces a protective immune response against malaria, can be used in live vaccine formulations for malaria. In specific embodiments, a Plasmodium-related peptide can be expressed as a fusion protein, for example, with a bacterial enterotoxin. The invention also relates to methods for expression of malaria antigens or fragments thereof within attenuated enteroinvasive bacteria. In particular embodiments, the invention is directed to the expression by attenuated Salmonella spp. of epitopes of Plasmodium circumsporozoite proteins.