Xiao Yong Wang

US Patent:

6824980, Nov 30, 2004

Filed:

May 23, 2001

Appl. No.:

09/862417

Inventors:

Xiao Bing Wang - Lutherville MD 21093

International Classification:

C12Q 168

US Classification:

435 6, 435 71, 435 911, 435 912, 4352872, 536221, 536 231, 536 243, 536 2431, 536 2432, 536 2433

Abstract:

A method for detecting and/or quantifying a target DNA or RNA present in a sample by a isometric primer extension method is disclosed. The method includes carrying out a primer extension reaction in the absence of a free nucleotide so that the primer extension reaction is stopped where the absent nucleotide would have been inserted. Thus, as the amount of incorporation of a labeled nucleotide on the primer extended product is detected, the amount of the target RNA or DNA in the sample is measured.

US Patent:

20030175220, Sep 18, 2003

Filed:

Dec 11, 2002

Appl. No.:

10/316269

Inventors:

Xiao Wang - Lutherville MD, US
Shinkatsu Morisawa - Kyoto, JP

International Classification:

A61K007/20

US Classification:

424/053000

Abstract:

The present invention provides compositions and methods for inhibiting, reducing, preventing or controlling dental caries formation. Electrolyzed water having an oxidation-reduction potential from about -150 to 0 mV measured against a platinum electrode, also known as “Trim water” can reduce, inhibit, prevent, or control caries in part by neutralizing dental plaque acid by increasing the pH in the area surrounding dental plaque. One embodiment provides contacting a tooth with electrolyzed water, typically after the tooth has been challenged with a sugar, to neutralize dental plaque acid and thereby reduce the formation of dental caries. Electrolyzed water and compositions comprising electrolyzed water can be formulated as palatable beverages, gels, candy, or other foodstuffs that are safely digested by individuals such as young children.

US Patent:

20040137497, Jul 15, 2004

Filed:

Jan 14, 2004

Appl. No.:

10/757263

Inventors:

Xiao Wang - Sparks MD, US

International Classification:

C12Q001/68
C12P019/34

US Classification:

435/006000, 435/091200

Abstract:

Methods, compositions, and kits for the detecting and/or quantification of polynucleotides are provided. An exemplary method provides hybridizing a first primer to the target polynucleotide and forming equal length primer extension products using a nucleotide reaction mixture consisting of X, Y, and Z, wherein X and Y are different purine non-terminator nucleotides, and Z is a pyrimidine non-terminator nucleotide, or X and Y are different pyrmidine non-terminator nucleotides, and Z is a purine non-terminator nucleotide. A portion of the extension products are hybridized to a second primer that is immobilized on a solid support, and the second primer is extended with at least one nucleotide having a detectable marker. The signal from the detectable marker can be correlated to the amount of target polynucleotide. Kits for performing this method are also provided.

US Patent:

20060110765, May 25, 2006

Filed:

Nov 18, 2005

Appl. No.:

11/282491

Inventors:

Xiao Wang - Lutherville MD, US

International Classification:

C12Q 1/68
C12P 19/34

US Classification:

435006000, 435091200

Abstract:

Methods and compositions for detecting nucleic acid polymorphisms are provided.

US Patent:

20060141503, Jun 29, 2006

Filed:

Nov 21, 2005

Appl. No.:

11/284453

Inventors:

Xiao Wang - Lutherville MD, US

Assignee:

SHINKATSU MORISAWA - NISHIKYO-KU

International Classification:

C12Q 1/68

US Classification:

435006000

Abstract:

The invention relates to a method for detecting any mutation at a predetermined site occurring in a known nucleic acid sequence. The method uses primer extension analysis to detect the mutation. Unlabeled terminator is supplied along with labeled non-terminator in the primer extension reaction to detect whether the first nucleic acid base on the template strand that is directly opposite the nucleic acid base immediately 3′ to a primer is a mutant. In the primer extension reaction, the terminator is complementary to the wild-type base on the template strand that is directly opposite the nucleic acid base immediately 3′ to the primer. Non-terminators are the other nucleotides and are labeled. When the terminator is incorporated into the primer extension strand, primer extension reaction terminates. Incorporation of a labeled non-terminator in the primer extension strand indicates that a mutation has occurred at the predetermined nucleic acid base site.

US Patent:

20210147834, May 20, 2021

Filed:

Nov 16, 2020

Appl. No.:

17/099093

Inventors:

Xiao Bing WANG - Sparks Glencoe MD, US
Xiao Fei LI - Sparks Glencoe MD, US

International Classification:

C12N 15/10
C12Q 1/6853

Abstract:

One embodiment provides methods to identify known and unknown gene fusions by creating a cDNA circle and analyzing the circle cDNA by amplification or sequencing. The circle cDNA is created in two approaches: 1) reverse transcribe a target RNA to cDNA, ligate the 3′end of the cDNA to its 5′ end to form a circle cDNA, or 2). ligate the 3′ end of target RNA to its 5′ end to form a circle RNA, reverse transcribe the RNA to a cDNA, and ligate the cDNA to form a circle cDNA. The circle cDNA is amplified using a primer designed from a known sequence of a wild type target gene by rolling circle amplification or PCR methods. The known or unknown fusion gene sequences in the circle cDNA are amplified and identified by sequencing analysis.

US Patent:

20180060485, Mar 1, 2018

Filed:

Aug 21, 2017

Appl. No.:

15/682240

Inventors:

- Indianapolis IN, US
- College Park MD, US
Haixu Tang - Bloomington IN, US
Xiao Wang - Greenbelt MD, US

International Classification:

G06F 19/28
G06F 19/00
G06F 21/62
G06F 19/22

Abstract:

Method including securely calculating, by a processor in electrical communication with a genome registry, an edit-distance between a query private genome and a second private genome, and securely calculating, by the processor, a set difference size between the query genome and the second private genome are disclosed. Systems capable of performing similar patient queries while preserving privacy are also disclosed.