Xiaohui Wang

US Patent:

20190185929, Jun 20, 2019

Filed:

Dec 10, 2018

Appl. No.:

16/215489

Inventors:

- South San Francisco CA, US
Tze Howe Charn - South San Francisco CA, US
Jason A. A. West - Pleasanton CA, US
Xiaohui Wang - Foster City CA, US

Assignee:

Fluidigm Corporation - South San Francisco CA

International Classification:

C12Q 1/6874
B01L 3/00
C12Q 1/6844

Abstract:

Described herein are cell-based analytic methods, including a method of incorporating nucleic acid sequences into reaction products from a cell population, wherein the nucleic acid sequences are incorporated into the reaction products of each cell individually or in small groups of cells individually. Also described herein is a matrix-type microfluidic device that permits at least two reagents to be delivered separately to each cell or group of cells, as well as primer combinations useful in the method and device.

US Patent:

20170315752, Nov 2, 2017

Filed:

Apr 27, 2016

Appl. No.:

15/140445

Inventors:

- MOUNTAIN VIEW CA, US
ANDREW KLEINERMAN - SAN FRANCISCO CA, US
BENJAMIN SCHOLBROCK - SUNNYVALE CA, US
TAHER VOHRA - SUNNYVALE CA, US
XIAOHUI WANG - SAN JOSE CA, US

International Classification:

G06F 3/06
G06F 3/06
G06F 3/06
G06F 3/06
G11C 11/4096
G11C 11/4074

Abstract:

Migrating data in a storage array that includes a plurality of storage devices, including: detecting, by the storage array, an occurrence of a storage device evacuation event associated with one or more source storage devices; responsive to detecting the occurrence of the storage device evacuation event, identifying, by the storage array, one or more target storage devices for receiving data stored on the one or more source storage devices; reducing, by the storage array, write access to the one or more source storage devices; and migrating the data stored on the one or more source storage devices to the one or more target storage devices.

US Patent:

20160251714, Sep 1, 2016

Filed:

Feb 26, 2016

Appl. No.:

15/055252

Inventors:

- South San Francisco CA, US
Tze Howe Charn - South San Francisco CA, US
Jason A. A. West - South San Francisco CA, US
Xiaohui Wang - South San Francisco CA, US

International Classification:

C12Q 1/68

Abstract:

Described herein are cell-based analytic methods, including a method of incorporating nucleic acid sequences into reaction products from a cell population, wherein the nucleic acid sequences are incorporated into the reaction products of each cell individually or in small groups of cells individually. Also described herein is a matrix-type microfluidic device that permits at least two reagents to be delivered separately to each cell or group of cells, as well as primer combinations useful in the method and device.

US Patent:

20150361486, Dec 17, 2015

Filed:

May 15, 2015

Appl. No.:

14/713887

Inventors:

- South San Francisco CA, US
Stacey N. Meyers - South San Francisco CA, US
Xiaohui Wang - South San Francisco CA, US
Jun Wang - South San Francisco CA, US

Assignee:

FLUIDIGM CORPORATION - South San Francisco CA

International Classification:

C12Q 1/68

Abstract:

The invention provides a method for detecting a target nucleotide sequence by tagging the nucleotide sequence with a nucleotide tag, providing a probe oligonucleotide with a melting temperature Tm1, comprising a regulatory sequence and a nucleotide tag recognition sequence; incorporating the probe oligonucleotide into the tagged polynucleotide in a polynucleotide amplification reaction, providing a regulatory oligonucleotide with a melting temperature Tm2, comprising a sequence segment that complementary to the regulatory sequence and a tail segment that does not hybridize to the probe nucleotide when the sequence segment and the regulatory sequence are annealed, amplifying the tagged target nucleic acid sequence in a PCR amplification reaction using the probe oligonucleotide as a primer, and using a DNA polymerase with high strand displacement activity and low 5′-nuclease activity, and detecting the amplification product; wherein Tm1 and Tm2 are higher than the annealing temperature associated with the polynucleotide amplification reaction.

US Patent:

20150337298, Nov 26, 2015

Filed:

May 21, 2015

Appl. No.:

14/719149

Inventors:

- South San Francisco CA, US
Xiaohui Wang - Foster City CA, US
Marc Unger - San Mateo CA, US
David Ruff - San Francisco CA, US

International Classification:

C12N 15/10
C12Q 1/68

Abstract:

In certain embodiments, the present invention provides a way of “digitally” marking different the alleles of different chromosomes by using a transposase to insert differently barcoded transposons into genomic DNA before further analysis. According to this method, each allele becomes marked with a unique pattern of transposon barcodes. Because each unique pattern of transposon barcodes identifies a particular allele, the method facilitates determinations of ploidy and copy number variation, improves the ability to discriminate among homozygotes, heterozygotes, and patterns arising from sequencing errors, and allows loci separated by uninformative stretches of DNA to be identified as linked loci, thereby facilitating haplotype determinations. Also provided is a novel artificial transposon end that includes a barcode sequence in two or more positions that are not essential for transposition.

US Patent:

20150147755, May 28, 2015

Filed:

Jul 24, 2014

Appl. No.:

14/340446

Inventors:

- South San Francisco CA, US
Stacey N. Myers - San Francisco CA, US
Jun Wang - Palo Alto CA, US
Xiaohui Wang - Foster City CA, US

International Classification:

C12Q 1/68

US Classification:

435 611

Abstract:

The invention provides a method for detecting a target nucleotide sequence by tagging the nucleotide sequence with a nucleotide tag, providing a probe oligonucleotide with a melting temperature Tm1, comprising a regulatory sequence and a nucleotide tag recognition sequence; incorporating the probe oligonucleotide into the tagged polynucleotide in a polynucleotide amplification reaction, providing a regulatory oligonucleotide with a melting temperature Tm2, comprising a sequence segment that is at least partially complementary to the regulatory sequence, amplifying the tagged target nucleic acid sequence in a PCR amplification reaction using the probe oligonucleotide as a primer, and detecting the amplification product; wherein Tm1 and Tm2 are higher than the annealing temperature associated with the polynucleotide amplification reaction.