Xing J Chen

US Patent:

20210372995, Dec 2, 2021

Filed:

Oct 22, 2018

Appl. No.:

16/758030

Inventors:

- Baltimore MD, US
David Gracias - Baltimore MD, US
Jayson Pagaduan - Windsor MD, US
Anil Bhatta - Baltimore MD, US
Xing Chen - Baltimore MD, US
Qianru Jin - Cambridge MA, US

International Classification:

G01N 33/50
C12N 5/077
C12N 5/071
B01L 3/00

Abstract:

In one aspect, provided is a composition (biomimetic composition) that includes a biomimetic in vitro model of an arteriolar vessel comprising: at least one of 1) human smooth muscle cells and 2) human pulmonary endothelial cells; wherein the vessel recapitulates one or more of the overall tubular geometry, morphometrics, extracellular matrix constituents, cellular morphology, cellular alignment, and functional heterotypic connections between the human smooth muscle cells and/or the human endothelial cells as compared to an in vivo arteriolar vessel. A microfluidics-based model platform of the pulmonary circulation is provided. Methods of use include measuring flow in biomimetic vessels, and to determine the resistance of these biomimetic vessels in the setting of a variety of experimental conditions that recapitulate the pathobiology of pulmonary hypertension.