Xinying Xie

US Patent:

6787312, Sep 7, 2004

Filed:

Aug 9, 2001

Appl. No.:

09/925808

Inventors:

Yijia Bao - Painted Post NY
Guangshan Li - Corning NY
Nalini Raghavachari - Painted Post NY
Mingqian He - Painted Post NY
Xinying Xie - Painted Post NY
Yuming Xie - Painted Post NY

Assignee:

Corning Incorporated - Corning NY

International Classification:

C12Q 168

US Classification:

435 6, 536 231, 536 243

Abstract:

A method of treating a substrate for immobilizing a biomolecule and substrates produced by the method are disclosed. The method includes contacting at least a portion of a substrate with a reducing agent such as a hydride. Treatment with an appropriate reducing agent substantially eliminates autofluorescence on substrates.

US Patent:

7141374, Nov 28, 2006

Filed:

Dec 5, 2003

Appl. No.:

10/729618

Inventors:

Yijia Bao - Painted Post NY, US
Guangshan Li - Corning NY, US
Nalini Raghavachari - Painted Post NY, US
Mingqian He - Painted Post NY, US
Xinying Xie - Painted Post NY, US
Yuming Xie - Painted Post NY, US

Assignee:

Corning Incorporated - Corning NY

International Classification:

C12Q 1/68
G01N 33/53

US Classification:

435 6, 435 71

Abstract:

A method of treating a substrate for immobilizing a biomolecule and substrates produced by the method are disclosed. The method includes contacting at least a portion of a substrate with a reducing agent such as a hydride. Treatment with an appropriate reducing agent substantially eliminates autofluorescence on substrates.

US Patent:

7364849, Apr 29, 2008

Filed:

Dec 5, 2003

Appl. No.:

10/729619

Inventors:

Yijia Bao - Painted Post NY, US
Guangshan Li - Corning NY, US
Nalini Raghavachari - Painted Post NY, US
Mingqian He - Painted Post NY, US
Xinying Xie - Painted Post NY, US
Yuming Xie - Painted Post NY, US

Assignee:

Corning Incorporated - Corning NY

International Classification:

C12Q 1/68
C07H 21/02
C07H 21/04

US Classification:

435 6, 536 231, 536 243

Abstract:

A method of treating a substrate for immobilizing a biomolecule and substrates produced by the method are disclosed. The method includes contacting at least a portion of a substrate with a reducing agent such as a hydride. Treatment with an appropriate reducing agent substantially eliminates autofluorescence on substrates.

US Patent:

20020172960, Nov 21, 2002

Filed:

Dec 20, 2001

Appl. No.:

10/028512

Inventors:

Yijia Bao - Painted Post NY, US
Ann Ferrie - Painted Post NY, US
Fang Lai - Painted Post NY, US
Thomas Volkert - Cambridge MA, US
Xinying Xie - Painted Post NY, US
Qin Zong - Germantown MD, US

International Classification:

C12Q001/68
C12M001/34

US Classification:

435/006000, 435/287200

Abstract:

A method for producing a DNA microarray for biological assays comprising a plurality of biosites located on a solid substrate. Each biosite having at least one set of networked oligonucleotides of medium to long lengths attached to the substrate by predominantly non-covalent means. The oligonucleotide strands are chemically presynthesized, exhibit no predetermined spatial orientation on the substrate surface, and have three-dimensional freedom of movement when in a hybridization solution. The oligonucleotides are chemically unmodified having a length of about 30 bases or longer. Preferably, the oligonucleotides are from about 45 bases to 85 bases, but some embodiments may include very long oligonucleotides of over 100 bases.

US Patent:

20030082618, May 1, 2003

Filed:

Oct 15, 2002

Appl. No.:

10/271193

Inventors:

Guangshan Li - Corning NY, US
Uwe Muller - Waukegan IL, US
Yijia Bao - DesPlaines IL, US
Xinying Xie - Painted Post NY, US
Nalini Raghavachari - Painted Post NY, US

International Classification:

C12Q001/68
C12P019/34
B05D003/00

US Classification:

435/006000, 435/091200, 427/002110

Abstract:

Methods and DNA microarray devices for detecting or measuring genetic aberrations or changes in genomic DNA using comparative genomic hybridization (CGH) techniques and gene-expression assays are provided.

US Patent:

20040043508, Mar 4, 2004

Filed:

Sep 3, 2002

Appl. No.:

10/234412

Inventors:

Anthony Frutos - Painted Post NY, US
Joydeep Lahiri - Painted Post NY, US
Thomas Leslie - Horseheads NY, US
Jinlin Peng - Painted Post NY, US
Dana C. Bookbinder - Corning NY, US
Xinying Xie - Painted Post NY, US

International Classification:

C12Q001/68
C12M001/34
G01N033/543

US Classification:

436/518000, 435/287200, 435/006000

Abstract:

A substrate, which that is capable of attaching biomolecules, and a method for preparing the substrate are provided. The substrate has a reactive surface that can covalently attach a polymer coating containing functional groups, which can reduce nonspecific binding of biomolecules to the surface for a biological array. Optionally, at least a portion of the substrate may be coated with an intermediate tie layer, which enhances the covalent bonding between the polymer coating with the underlying substrate. The present invention also pertains to a method that uses electrostatic blocking agents to reduce non-specific binding of proteins to a substrate, especially anhydride-modified surfaces.

US Patent:

20050136413, Jun 23, 2005

Filed:

Dec 22, 2003

Appl. No.:

10/745835

Inventors:

Michael Briggs - Hampton NH, US
Thomas Bunch - Painted Post NY, US
Andrew Ferrie - Painted Post NY, US
Xinying Xie - Painted Post NY, US

International Classification:

C12Q001/68

US Classification:

435006000

Abstract:

A reagent system for printing, assaying, and processing nucleic acid microarrays is provided. The system comprises: a printing kit, which includes a nucleic acid spotting solution; and a hybridization kit, which includes a nucleic acid pre-hybridization solution, a nucleic acid hybridization solution, and first, second, and third wash reagents, wherein the respective constituent components of the printing and hybridization kits are stable and retain functional performance, when stored together at a temperature between about 10 C. to about 50 C. A background reducing agent or solution is also included. The present reagent solutions are optimized for use with glass substrates having preferably an amine-coating, such as GAPS.

US Patent:

6391655, May 21, 2002

Filed:

Jul 29, 1998

Appl. No.:

09/124352

Inventors:

Dana C. Bookbinder - Corning NY
Leroy S. Hersh - Painted Post NY
Xinying Xie - Painted Post NY

Assignee:

Corning Incorporated - Corning NY

International Classification:

G01N 33545

US Classification:

436531, 435 6

Abstract:

A method of immobilizing aminated DNA to a substrate surface is provided for use in DNA hybridization assays. The surface can be widely employed in a variety of molded laboratory products including multiwell plates, cell culture dishes, and biological containers. The method comprises the steps of providing a polystyrene substrate having a surface content of between 0. 3 and 4. 0% atomic percent oxygen, and attaching amine modified oligonucleotide to the surface of the substrate. A resultant product comprising a polystyrene substrate having a surface oxygen content of between 0. 3 and 4. 0% atomic percent, and a plurality of amine modified oligonucleotides directly attached to the surface of the substrate, is also disclosed.