Skills:
Protein biology and Biochemistry: Expression and extraction from E.coli and mammalian cell system, purification using ultra centrifugation, gel filtration and affinity binding column and HPLC methods, protein analysis and detection by ELISA, IEF, SDS-PAGE and western blotting, phosphatase assay. Virology and Immunology: Isolation, cultivation, purification and identification of wild strain virus, viral identification using serological, cytological and molecular biology methods, vaccine production, antibody conjugation, immunofluorescence, immunoblotting, ELISA. Molecular Biology: CHIP assay, cloing, PCR, qPCR, site-directed mutation, northern blotting, southern blotting, gene knockout and knock-in using recombineering. Cell Biology: Cell culture (include cell lines, ES cells and primary cells), differentiation, cell growth, proliferation, apoptosis, adhesion assay, transfection using shRNA or siRNA, gene over expression or knock down using siRNA, shRNA or lenti-virus (AAV) or adenovirus on primary cells, AAV production. Animals: Knockout and transgenic mice, subcutaneous pump implantation Microscopy: Light microscopy, fluorescent/laser microscopy