Yen S Liu

US Patent:

20030165917, Sep 4, 2003

Filed:

Aug 14, 2002

Appl. No.:

10/219195

Inventors:

Edwin Ullman - Atherton CA, US
Ming Wu - Castro Valley CA, US
Yen Liu - Cupertino CA, US

International Classification:

C12Q001/68
C12P019/34

US Classification:

435/006000, 435/091200

Abstract:

A method for detection of nucleic acid targets using as reagents: (1) a stem loop probe having a long strand for hybridizing to a target, a short strand, usually with a free 3′-end for extension, hybridized to a portion of the long strand and a linker forming a loop and joining the short and long strands; and (2) a hybridizing reagent that hybridizes to the short strand when released by the target. The target is detected by extension of one or both the probe or hybridizing reagent along each other. A circular hybridizing reagent can be employed with DNA polymerase for concatenated extension of the probe 3′-end or extension of the 3′-end of the probe along a hybridizing reagent having a promoter sequence for forming transcripts of at least a portion of the long strand or the hybridizing reagent. A non-cleavable restriction site consensus sequence in the linker, where the hybridizing reagent is extended with dNTPs and DNA polymerase and the extended sequence is cleaved with a restriction enzyme, so that chains may be repetitively produced and cleaved. The presence of the target nucleic acid is established by detecting the concatenated chain, the RNA transcripts or the repetitively produced chains.

US Patent:

62007572, Mar 13, 2001

Filed:

Jan 19, 1999

Appl. No.:

9/233413

Inventors:

Nurith Kurn - Palo Alto CA
Yen Ping Liu - Cupertino CA
Alla Lishanski - San Jose CA
Marc Taylor - Mountain View CA

Assignee:

Dade Behring Inc. - Deerfield IL

International Classification:

C12Q 168

US Classification:

435 6

Abstract:

The present invention relates to a method for selectively extending an oligonucleotide primer along a specific target polynucleotide sequence in a mixture of polynucleotides. A combination is provided comprising the mixture, an oligonucleotide primer having a modification, and a binding substance for the modification wherein the binding substance binds to the oligonucleotide and prevents the extension of the oligonucleotide along the target polynucleotide sequence. The temperature of the combination is adjusted to a level sufficient to irreversibly denature the binding substance and permit the extension of the oligonucleotide primer along the specific target polynucleotide sequence. The invention has particular application in the amplification of nucleic acids. Also disclosed are kits for carrying out a method in accordance with the present invention.

US Patent:

51870665, Feb 16, 1993

Filed:

Feb 14, 1990

Appl. No.:

7/479930

Inventors:

Martin Becker - Palo Alto CA
Nurith Kurn - Palo Alto CA
Yen P. Liu - Cupertino CA
Rajesh D. Patel - Fremont CA
Thomas M. Houts - Mountain View CA
John D. Olson - Sunnyvale CA

Assignee:

Syntex (U.S.A.) Inc. - Palo Alto CA

International Classification:

C12Q 100
G01N 33545

US Classification:

435 736

Abstract:

This invention is to a method for detecting an amphiphilic antigen in a biological sample suspected of containing the amphiphilic antigen, which method comprises providing in combination a hydrophilic solid support modified to have a hydrophobic surface and an assay medium suspected of containing an amphiphilic antigen, incubating the combination under conditions sufficient for the amphiphilic antigen to bind to the hydrophobic surface, and determining the presence or amount of the amphiphilic antigen bound to the hydrophobic surface.