Yi H Liu

US Patent:

8562809, Oct 22, 2013

Filed:

Oct 2, 2009

Appl. No.:

13/122403

Inventors:

Yi Liu - Bethesda MD, US
Gregory F. Payne - Hunt Valley MD, US
W. Lee Meyer - Baltimore MD, US

Assignee:

University of Maryland, College Park - College Park MD

International Classification:

C25D 9/02

US Classification:

205229, 205317

Abstract:

A method of forming a bioelectronic device including a protein on an electrically conductive substrate, by electrodepositing aminopolysaccharide chitosan on the substrate while applying a cathodic voltage to the substrate, to form an aminopolysaccharide chitosan film thereon, applying an anodic voltage to the substrate in the presence of NaCl to activate the aminopolysaccharide chitosan film so that it is reactive with protein. The method also optionally includes reacting the aminopolysaccharide film, after activation thereof, with the protein, so that the protein assembles on and is coupled to the substrate, thereby forming a bioelectronic device. The protein can include single or multiple protein species, and including biosensing proteins. Additional methods include biosensing of electrochemically active compounds either present in a sample or generated during a biological recognition event and devices useful in such methods. The resulting devices are useful as sensors in hand-held devices, textiles, garments and the like.

US Patent:

20040010119, Jan 15, 2004

Filed:

Feb 12, 2002

Appl. No.:

10/074978

Inventors:

Xiaojia Guo - Branford CT, US
Elma Fernandes - Branford CT, US
Li Li - Branford CT, US
Ramesh Kekuda - Stamford CT, US
Yi Liu - New Haven CT, US
Mario Leite - Milford CT, US
Kimberly Spytek - New Haven CT, US
Weizhen Ji - Branford CT, US
Stacie Casman - North Haven CT, US
Ference Boldog - North Haven CT, US
Meera Patturajan - Branford CT, US
Corine Vernet - Branford CT, US
Robert Ballinger - Newington CT, US
Uriel Malyankar - Branford CT, US
Velizar Tchernev - Branford CT, US
Angela Blalock - Branford CT, US
Vladimir Gusev - Madison CT, US
Luca Rastelli - Guilford CT, US
Peter Mezes - Old Lyme CT, US
Karen Ellerman - Branford CT, US
Melvyn Heyes - New Haven CT, US
John Herrmann - Guilford CT, US
Richard Shimkets - Guilford CT, US
Noelle Ioime - Hamden CT, US
Carol Pena - New Haven CT, US
Suresh Shenoy - Branford CT, US
Raymond Taupier - East Haven CT, US
Valerie Gerlach - Branford CT, US
Linda Gorman - East Haven CT, US

International Classification:

C12Q001/68
C07H021/04
A61K038/17
C07K014/435
C07K014/47
C12P021/02
C12N005/06

US Classification:

530/350000, 514/012000, 435/006000, 435/069100, 435/320100, 435/325000, 536/023200

Abstract:

Disclosed herein are nucleic acid sequences that encode novel polypeptides. Also disclosed are polypeptides encoded by these nucleic acid sequences, and antibodies, which immunospecifically-bind to the polypeptide, as well as derivatives, variants, mutants, or fragments of the aforementioned polypeptide, polynucleotide, or antibody. The invention further discloses therapeutic, diagnostic and research methods for diagnosis, treatment, and prevention of disorders involving any one of these novel human nucleic acids and proteins.

US Patent:

20040043382, Mar 4, 2004

Filed:

Mar 7, 2002

Appl. No.:

10/092900

Inventors:

Muralidhara Padigaru - Branford CT, US
Kimberly Spytek - New Haven CT, US
Suresh Shenoy - Branford CT, US
Raymond Taupier - East Haven CT, US
Carol Pena - New Haven CT, US
Li Li - Branford CT, US
Bryan Zerhusen - Branford CT, US
Vladimir Gusev - Madison CT, US
Weizhen Ji - Branford CT, US
Linda Gorman - Branford CT, US
Charles Miller - Guilford CT, US
Ramesh Kekuda - Norwalk CT, US
Meera Patturajan - Branford CT, US
Esha Gangolli - Madison CT, US
Corine Vernet - Branford CT, US
Xiaojia Guo - Branford CT, US
Velizar Tchernev - Branford CT, US
Elma Fernandes - Branford CT, US
Stacie Casman - North Haven CT, US
Uriel Malyankar - Branford CT, US
Valerie Gerlach - Branford CT, US
Yi Liu - San Diego CA, US
David Anderson - Branford CT, US
Steven Spaderna - Berlin CT, US
Elina Catterton - Madison CT, US
Mario Leite - Milford CT, US
Haihong Zhong - Guilford CT, US
John Alsobrook - Madison CT, US
Denise Lepley - Branford CT, US
Daniel Rieger - Branford CT, US
Catherine Burgess - Wethersfield CT, US

International Classification:

C12Q001/68
C07H021/04
C12N009/00
C12P021/02
C12N005/06
C07K014/47

US Classification:

435/006000, 435/069100, 435/183000, 435/320100, 435/325000, 530/350000, 536/023200

Abstract:

The present invention provides novel isolated polynucleotides and small molecule target polypeptides encoded by the polynucleotides. Antibodies that immunospecifically bind to a novel small molecule target polypeptide or any derivative, variant, mutant or fragment of that polypeptide, polynucleotide or antibody are disclosed, as are methods in which the small molecule target polypeptide, polynucleotide and antibody are utilized in the detection and treatment of a broad range of pathological states. More specifically, the present invention discloses methods of using recombinantly expressed and/or endogenously expressed proteins in various screening procedures for the purpose of identifying therapeutic antibodies and therapeutic small molecules associated with diseases.

US Patent:

20120103822, May 3, 2012

Filed:

Apr 27, 2010

Appl. No.:

13/266433

Inventors:

Yi Liu - Bethesda MD, US
Gregory F. Payne - Hunt Valley MD, US
Xiaohua Yang - Ardmore OK, US

Assignee:

UNIVERSITY OF MARYLAND COLLEGE PARK - College Park MD

International Classification:

C25D 9/02
G01N 33/53
C12N 13/00

US Classification:

205317, 4351731, 435 71

Abstract:

Methods for the generation of hydrogels formed by electrodeposition of an electroaddressable polymer are described. The hydrogels may contain one or more cell populations electroaddressed or electroaddressable to a location within the hydrogel and where the cells of the cell populations are entrapped by the hydrogel and are capable of expansion within the hydrogel and may be releasable from the hydrogel. Further provided are electroaddressable polysaccharide blends for the in-film expansion of a cell population, allowing probing of the cells and formation of immunocomplexes. Further provided are methods of using hydrogels containing electroaddressed or electroaddressable cell populations in in-film bioprocessing methods such as cell-based biosensing, protein-based biosensing, and in studies of cell signaling.

US Patent:

20190167116, Jun 6, 2019

Filed:

Nov 15, 2018

Appl. No.:

16/192229

Inventors:

Yu Chen - College Park MD, US
Qinggong Tang - College Park MD, US
Udayakumar Kanniyappan - Hyattsville MD, US
Lin Z. Li - Aston PA, US
He Xu - Lansdowne PA, US
Yi Liu - College Park MD, US
Emily Conant - Philadelphia PA, US

International Classification:

A61B 5/00
A61B 1/07
A61B 1/04
A61B 1/06
A61B 1/00
A61B 10/02

Abstract:

In accordance with aspects of the present disclosure, an exemplary system includes a hollow probe having a lumen containing one or more excitation optical fiber(s) and one or more imaging optical fiber(s) where the probe is sized to access a person's body, a first light source optically coupled to the excitation fiber(s) and configured to emit light that excites fluorescence of NADH in breast tissue, a second light source optically coupled to the excitation fiber(s) and configured to emit light that excites fluorescence of FAD in tissue such as breast tissue, an image capturing device optically coupled to the imaging fiber(s), and a controller configured to control the first light source and the image capturing device to capture NADH fluorescence signals/intensities while the probe is within the person's body and control the second light source and the image capturing device to capture FAD fluorescence signals/intensities while the probe is within the person's body.