Parsons Brinckerhoff / Ammann & Whitney New York, NY Oct 2014 to Feb 2015 Junior Engineer / Senior InspectorMugar Library Circulation Desk Boston, MA Sep 2011 to May 2014 Library AssistantBoston University Student-satellite Applications and Training Boston, MA Jul 2013 to Sep 2013 Mechanical Design StudentBoston University China Care Fund Boston, MA Feb 2012 to Oct 2012 Fundraising CoordinatorRadisson Martinique Hotel New York, NY Jul 2012 to Sep 2012 Housekeeping Management Intern
Education:
Boston University Boston, MA 2014 Bachelor of Science in Mechanical EngineeringBronx High School of Science Bronx, NY 2010 High School Diploma
Dana-Farber Cancer Institute, Harvard Medical School
2009 to 2000 Research Technician at Department of Cancer Immunology and AIDSThe University of Hong Kong Hong Kong, Hong Kong Island 2007 to 2009 Master student and Research Assistant II at Department of Paediatrics & Adolescent MedicineDepartment of Chemistry, University of Massachusetts Boston Boston, MA 2005 to 2006 Research student
Education:
The University of Hong Kong Hong Kong, Hong Kong Island 2007 to 2009 M.Phil in Immunology and infectious diseaseUniversity of Massachusetts Boston Boston, MA 2001 to 2006 B.S in Biochemistry
Skills:
Virology and cell biology expertise In vitro HIV/SHIV replication assays using replication competent HIV/SHIV isolates Virus culture and propagation in primary cells such as PBMCs Virus titration and stock preparation Transformation, inoculation and ligation of virus plasmids Maintenance of cell lines Transfection of virus clones into human cell lines Molecular Biology and Immunology expertise DNA and RNA isolation DNA and RNA purification Real time PCR and Q-PCR DNA digestion (restriction enzyme) T cells and Monocytes isolation by magnetic beads from whole blood and primary cell cultures immunofluorescence staining and intracellular staining flow cytometry, spectrophotometry and confocal microcopy ELISA Immunoblot Neutralization assays Chemistry expertise thin layer chromatography HPLC-MS