Yong Yao

US Patent:

6818747, Nov 16, 2004

Filed:

Oct 29, 2001

Appl. No.:

09/984292

Inventors:

Yong Yao - San Diego CA
Hong Xu - San Diego CA

Assignee:

Senomyx, Inc. - La Jolla CA

International Classification:

C07K 1900

US Classification:

530350, 435 697, 435536, 536 234

Abstract:

The invention provides a series of G protein variants that functionally couple to sensory cell receptors such as taste GPCRs (TRs) and olfactory GPCRs (ORs) in an overly promiscuous manner. According to the invention, the functional coupling can be determined, for example, by measuring changes in intracellular IP3, or calcium. In a particular embodiment, the G protein variants can be expressed in mammalian cell lines or Xenopus oocytes, and then evaluated using calcium fluorescence imaging and electrophysiological recording.

US Patent:

7041457, May 9, 2006

Filed:

Nov 21, 2001

Appl. No.:

09/989497

Inventors:

Yong Yao - San Diego CA, US
Hong Xu - San Diego CA, US

Assignee:

Senomyx, Inc. - La Jolla CA

International Classification:

G01N 33/53

US Classification:

435 71, 435 72, 435 721, 435 78

Abstract:

The invention provides a series of Gαprotein variants that functionally couple to sensory cell receptors such as taste GPCRs (TRs) and olfactory GPCRs (ORs) in an overly promiscuous manner. According to the invention, the functional coupling can be determined, for example, by measuring changes in intracellular IP3, or calcium. In a particular embodiment, the Gαprotein variants can be expressed in mammalian cell lines or oocytes, and then evaluated using calcium fluorescence imaging and electrophysiological recording.

US Patent:

7115377, Oct 3, 2006

Filed:

Mar 4, 2002

Appl. No.:

10/087217

Inventors:

Yong Yao - Gaithersburg MD, US
Liang Cao - Bethesda MD, US

Assignee:

Atto Bioscience, Inc. - Rockville MD

International Classification:

G01N 33/53
G01N 33/566

US Classification:

435 71, 435 72, 435 721, 436501

Abstract:

Disclosed are compositions and methods for their use, such as in identifying G-protein-coupled receptors, ligands and compounds that modulate the activities of G-protein-coupled receptors. The compositions and methods employ cyclic nucleotide-gated channels and fluorescence dyes in detecting changes of intracellular cAMP levels in response to the stimulation of G-protein-coupled receptors. Activation of the G-protein-coupled receptors can be detected in a variety of assays, including cell-based imaging assays with fluorescence microscopes and high throughput assays with multi-well plates and fluorescence plate readers.

US Patent:

7238213, Jul 3, 2007

Filed:

Nov 1, 2004

Appl. No.:

10/978282

Inventors:

Yong Yao - North Potomac MD, US
Liang Cao - Bethesda MD, US
Jianming Lu - Potomac MD, US
Isabel Llorente - Adamstown MD, US

Assignee:

Atto Bioscience - Rockville MD

International Classification:

C09B 51/00
A61B 5/0275
G01N 33/567

US Classification:

8667, 8648, 43027019, 435 71, 435 72, 435 721

Abstract:

Disclosed are novel compositions and methods employing cyclic nucleotide-gated channels and fluorescence dyes and other indicators in detecting changes of intracellular cAMP levels, for instance in response to the stimulation of G-protein-coupled receptors. CNG mutants comprising one or more pore mutations that enhance sensitivity to cAMP and decrease divalent cation-mediated blockage are used in a variety of assays, including detection of the G-protein-coupled receptor activity. Also disclosed are novel dye quencher formulations that are particularly suited for cell-based assays of protein interactions.

US Patent:

7291485, Nov 6, 2007

Filed:

Sep 30, 2004

Appl. No.:

10/952773

Inventors:

Yong Yao - San Diego CA, US
Hong Xu - San Diego CA, US

Assignee:

Senomyx, Inc. - San Diego CA

International Classification:

C12N 15/62

US Classification:

435 697, 4352523, 4353201, 536 234

Abstract:

The invention provides a series of Gαprotein variants that functionally couple to sensory cell receptors such as taste GPCRs (TRs) and olfactory GPCRs (ORs) in an overly promiscuous manner. According to the invention, the functional coupling can be determined, for example, by measuring changes in intracellular IP3, or calcium. In a particular embodiment, the Gαprotein variants can be expressed in mammalian cell lines or oocytes, and then evaluated using calcium fluorescence imaging and electrophysiological recording.

US Patent:

7384755, Jun 10, 2008

Filed:

Jul 25, 2006

Appl. No.:

11/492216

Inventors:

Yong Yao - Gaithersburg MD, US
Liang Cao - Bethesda MD, US

Assignee:

Atto Bioscience, Inc. - Rockville MD

International Classification:

G01N 33/53
G01N 33/566

US Classification:

435 71, 435 72, 435 721, 436501

Abstract:

Disclosed are compositions and methods for their use, such as in identifying G-protein-coupled receptors, ligands and compounds that modulate the activities of G-protein-coupled receptors. The compositions and methods employ cyclic nucleotide-gated channels and fluorescence dyes in detecting changes of intracellular cAMP levels in response to the stimulation of G-protein-coupled receptors. Activation of the G-protein-coupled receptors can be detected in a variety of assays, including cell-based imaging assays with fluorescence microscopes and high throughput assays with multi-well plates and fluorescence plate readers.

US Patent:

7604959, Oct 20, 2009

Filed:

Apr 12, 2007

Appl. No.:

11/783884

Inventors:

Yong Yao - North Potomac MD, US
Liang Cao - Bethesda MD, US
Jianming Lu - Potomac MD, US
Isabel Llorente - Adamstown MD, US

Assignee:

Atto BioScience Inc. - Rockville MD

International Classification:

C12N 15/12
C07K 14/705

US Classification:

435 691, 435 71, 435 72, 4352523, 4353201, 536 235

Abstract:

Disclosed are novel compositions and methods employing cyclic nucleotide-gated channels and fluorescence dyes and other indicators in detecting changes of intracellular cAMP levels, for instance in response to the stimulation of G-protein-coupled receptors. CNG mutants comprising one or more pore mutations that enhance sensitivity to cAMP and decrease divalent cation-mediated blockage are used in a variety of assays, including detection of the G-protein-coupled receptor activity. Also disclosed are novel dye quencher formulations that are particularly suited for cell-based assays of protein interactions.

US Patent:

7897386, Mar 1, 2011

Filed:

May 21, 2008

Appl. No.:

12/124717

Inventors:

Yong Yao - Gaithersburg MD, US
Liang Cao - Bethesda MD, US

Assignee:

Atto Bioscience Inc. - Rockville MD

International Classification:

C12N 5/10
C07K 14/705

US Classification:

435325, 435 721, 435 691

Abstract:

Disclosed are compositions and methods for their use, such as in identifying G-protein-coupled receptors, ligands and compounds that modulate the activities of G-protein-coupled receptors. The compositions and methods employ cyclic nucleotide-gated channels and fluorescence dyes in detecting changes of intracellular cAMP levels in response to the stimulation of G-protein-coupled receptors. Activation of the G-protein-coupled receptors can be detected in a variety of assays, including cell-based imaging assays with fluorescence microscopes and high throughput assays with multi-well plates and fluorescence plate readers.