Michael G. Murray - Madison WI Jane H. Cramer - Madison WI Jeanne Romero-Severson - Mazomainie WI David West - Prescott WI Yu Ma - Madison WI
A set of nucleic acid probes useful for tracking Maize Dwarf Mosaic Virus Resistance (MDMV), a polygenic trait, is provided. Chromosome segments are identified enabling isolation of the genes governing this trait.
A general method for identifying probes useful for tracking and introgressing polygenic traits into elite genomes and identifying chromosome segments governing the traits is also provided. This method involves the analysis of RFLP polymorphisms between parent donor and recipient genotypes and observed phenotypic data using multiple regression by leaps and bounds ("leaps"), followed by standard multiple regression applied to the "leaps" data. The "leaps" data may be used to identify flanking markers, and epistasis may be determined by analysis of the multiple regression data.
Kits comprising a subset of the most useful probes (those most closely linked to the trait of interest) are provided. The kits may also comprise flanking probes.