Yury Goltsev from Menlo Park, CA, age 52

Resumes

At Baxter Lab, Stanford, Department Of Microbiology And Immunology

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Position:

Research Scientist at Stanford University School of Medicine

Location:

San Francisco Bay Area

Industry:

Biotechnology

Work:

Stanford University School of Medicine since Jul 2009
Research Scientist
UC Berkeley Jul 2003 - Jun 2009
postdoc
Weizmann Institute of Science Jun 1995 - Jun 2003
Graduate Research Fellow

Education:

Weizmann Institute of Science 1995 - 2003
PhD, Molecular Immunology Moscow Institute of Physics and Technology (State University) (MIPT) 1989 - 1995
520 1987 - 1989

Skills:

Molecular Biology
Cell
Western Blotting
Tissue Culture
Bioinformatics
Biochemistry
DNA

Honor & Awards:

Patents 1. United States Patent No.: US 6,586,571 B1 2. Patent Application Publication No.: US 2005/0152877 A1 Recent Publications 1: Papatsenko D, Levine M, Goltsev Y. PLoS Biol. 2011 Jan 25;9(1) 2: Oh ST, Simonds EF, Jones C, Hale MB, Goltsev Y, Gibbs KD Jr, Merker JD, Zehnder JL, Nolan GP, Gotlib J. Blood. 2010 Aug 12;116(6):988-92 3: Goltsev Y, Papatsenko D. BMC Bioinformatics. 2009 Oct 26;10:353. 4: Cande J, Goltsev Y, Levine MS. Proc Natl Acad Sci U S A. 2009 Aug 25;106(34):14414-9 5: Papatsenko D, Goltsev Y, Levine M. Nucleic Acids Res. 2009 Sep;37(17):5665-77. 6: Goltsev Y, Rezende GL, Vranizan K, Lanzaro G, Valle D, Levine M. Dev Biol. 2009 Jun 15;330(2):462-70. 7: Goltsev Y, Fuse N, Frasch M, Zinzen RP, Lanzaro G, Levine M. 2007 Jul;134(13):2415-24 8: Goltsev Y, Hsiong W, Lanzaro G, Levine M. Dev Biol. 2004 Nov 15;275(2):435-46.

Us Patents

Use Of Jam-A In Diagnosing And Treating Leukemia
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US Patent:

20130287790, Oct 31, 2013
Filed:

Mar 14, 2013
Appl. No.:

13/829229
Inventors:

Garry Nolan - San Francisco CA, US
Yury Goltsev - Stanford CA, US
International Classification:

C12Q 1/68
US Classification:

4241531, 435 611, 435 723, 435 29, 506 9, 4241781, 424649
Abstract:

Methods, compositions, and kits are provided for the use of JAM-A in diagnosing and treating leukemia. These methods, compositions, and kits find many uses, for example in diagnosing an individual with a leukemia, classifying a leukemia, providing a prognosis to an individual with a leukemia, treating an individual with a leukemia, screening candidate agents for the ability to treat a leukemia, and in basic research to better understand the molecular and cellular basis of leukemia.

Highly-Multiplexed Fluorescent Imaging
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US Patent:

20220267827, Aug 25, 2022
Filed:

Oct 11, 2021
Appl. No.:

17/498627
Inventors:

- Stanford CA, US
Nikolay Samusik - Mountain View CA, US
Yury Goltsev - STANFORD CA, US
International Classification:

C12Q 1/6804
C12Q 1/6813
G01N 33/53
G01N 1/30
G01N 33/543
G01N 33/554
Abstract:

Provided herein is a method and system for analyzing a sample. In some embodiments the method makes use of a plurality of capture agents that are each linked to a different oligonucleotide and a corresponding plurality of labeled nucleic acid probes, wherein each of the labeled nucleic acid probes specifically hybridizes with only one of the oligonucleotides. The sample is labeled with the capture agents en masse, and sub-sets of the capture agents are detected using iterative cycles using corresponding subsets of the labeled nucleic acid probes.

On-Slide Staining By Primer Extension
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US Patent:

20230002809, Jan 5, 2023
Filed:

Feb 15, 2022
Appl. No.:

17/672552
Inventors:

- Stanford CA, US
Garry P. Nolan - Redwood City CA, US
Yury Goltsev - Stanford CA, US
International Classification:

C12Q 1/6818
C12Q 1/6804
Abstract:

A method for analyzing planar sample is provided. In some cases the method comprises: (a) incubating the planar sample with a capture agent that is linked to an oligonucleotide, wherein the capture agent specifically binds to complementary sites in the planar sample; (b) reading a fluorescent signal caused by extension of a primer that is hybridized to the oligonucleotide, using fluorescence microscopy. Several implementations of the method, and multiplexed versions of the same, are also provided.

Multiplexed Single Molecule Rna Visualization With A Two-Probe Proximity Ligation System
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US Patent:

20210238665, Aug 5, 2021
Filed:

Apr 14, 2021
Appl. No.:

17/230706
Inventors:

- Stanford CA, US
Felice Alessio Bava - Menlo Park CA, US
Yury Goltsev - Stanford CA, US
Garry P. Nolan - Redwood City CA, US
International Classification:

C12Q 1/6841
C12Q 1/682
G01N 33/542
Abstract:

SNAIL provides cost-efficient detection of specific nucleic acids in single cells, and may be combined with flow cytometry to simultaneously analyze large numbers of cells for a plurality of nucleic acids, e.g. at least one, to up to 5, up to 10, up to 15, up to 20 or more transcripts can be simultaneously analyzed, at a rate of up to about 50, 100, 250, 500 or more cells/second. The methods require only two primers for amplification, and may further include a detection primer.

Detection Of Target Nucleic Acids In A Cellular Sample
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US Patent:

20200385788, Dec 10, 2020
Filed:

May 13, 2020
Appl. No.:

15/930891
Inventors:

- Stanford CA, US
Yury Goltsev - Stanford CA, US
Quan Nguyen - San Ramon CA, US
Yunqing Ma - San Jose CA, US
Chunfai Lai - Fremont CA, US
International Classification:

C12Q 1/6813
C12Q 1/6841
Abstract:

Methods of assaying cells of a cellular sample for the presence of a target nucleic acid are provided. Aspects of the methods include evaluating a cellular sample that has been contacted with a nuclease inhibitor for the presence of a target nucleic acid. Also provided are devices and kits that find use in practicing the methods described herein.

On-Slide Staining By Primer Extension
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US Patent:

20200063187, Feb 27, 2020
Filed:

Nov 11, 2019
Appl. No.:

16/679769
Inventors:

- Stanford CA, US
Garry P. Nolan - Redwood City CA, US
Yury Goltsev - Stanford CA, US
David Robert McIlwain - Palo Alto CA, US
International Classification:

C12Q 1/6804
Abstract:

A method for analyzing planar sample is provided. In some cases the method comprises: (a) labelling the planar sample with a capture agent that is linked to a nucleic acid, wherein the capture agent specifically binds to complementary sites in the planar sample; (b) reading a fluorescent signal caused by extension of a primer that is hybridized to the nucleic acid, using fluorescence microscopy. Several implementations of the method, and multiplexed versions of the same, are also provided.

Highly-Multiplexed Fluorescent Imaging
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US Patent:

20200024641, Jan 23, 2020
Filed:

Jun 14, 2019
Appl. No.:

16/442349
Inventors:

- Stanford CA, US
Nikolay Samusik - Mountain View CA, US
Yury Goltsev - STANFORD CA, US
International Classification:

C12Q 1/6804
C12Q 1/6813
G01N 33/53
G01N 1/30
G01N 33/543
G01N 33/554
Abstract:

Provided herein is a method and system for analyzing a sample. In some embodiments the method makes use of a plurality of capture agents that are each linked to a different oligonucleotide and a corresponding plurality of labeled nucleic acid probes, wherein each of the labeled nucleic acid probes specifically hybridizes with only one of the oligonucleotides. The sample is labeled with the capture agents en masse, and sub-sets of the capture agents are detected using iterative cycles using corresponding subsets of the labeled nucleic acid probes.

On-Slide Staining By Primer Extension
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US Patent:

20190330689, Oct 31, 2019
Filed:

Jan 4, 2019
Appl. No.:

16/240463
Inventors:

- Stanford CA, US
Garry P. Nolan - Redwood City CA, US
Yury Goltsev - Stanford CA, US
International Classification:

C12Q 1/6818
C12Q 1/6804
Abstract:

A method for analyzing planar sample is provided. In some cases the method comprises: (a) incubating the planar sample with a capture agent that is linked to an oligonucleotide, wherein the capture agent specifically binds to complementary sites in the planar sample; (b) reading a fluorescent signal caused by extension of a primer that is hybridized to the oligonucleotide, using fluorescence microscopy. Several implementations of the method, and multiplexed versions of the same, are also provided.