- Hercules CA, US Andrew KOHLWAY - Pleasanton CA, US Jennifer CHEW - Sunnyvale CA, US Zachary BURKETT - Walnut Creek CA, US Man CHENG - Danville CA, US
International Classification:
C12Q 1/6869 G16B 40/10 G16B 30/10
Abstract:
Comparison of common sequencing reads from sequencing based on partition-based barcoding can be used to improve sequencing results. Increased loading of barcodes per partition can also improve sequencing results.
- Hercules CA, US Zachary BURKETT - Walnut Creek CA, US Man CHENG - Danville CA, US Meiye WU - Tracy CA, US Pranav PATEL - Fremont CA, US Duc DO - San Jose CA, US Arkadiusz BIBILLO - Walnut Creek CA, US
International Classification:
C12Q 1/6834 C12Q 1/6874 C12Q 1/6876 C12N 15/10
Abstract:
Provided are methods for detecting the presence of multiple barcoded solid supports in partitions, the method comprising providing a plurality of partitions wherein at least some partitions comprises multiple solid supports, where each solid support is linked to different solid support oligonucleotides, the solid support oligonucleotides comprising a barcode unique for the solid support; synthesizing a complementary sequence of the oligonucleotides to produce a double-stranded polynucleotide having barcodes from two of the solid support oligonucleotides; and sequencing one or both strands of the double-stranded polynucleotides, wherein sequences comprising two different barcodes indicates the presence of two solid supports in the same partition. Also provided are compositions and methods for producing the oligonucleotides.
Nucleotide Sequence Generation By Barcode Bead-Colocalization In Partitions
- Hercules CA, US Andrew KOHLWAY - Pleasanton CA, US Jennifer CHEW - Sunnyvale CA, US Zachary BURKETT - Walnut Creek CA, US Man CHENG - Danville CA, US
International Classification:
C12Q 1/6869 G16B 40/10 G16B 30/10
Abstract:
Comparison of common sequencing reads from sequencing based on partition-based barcoding can be used to improve sequencing results. Increased loading of barcodes per partition can also improve sequencing results.