The present invention relates to FTY720 analogs bearing a boron dipyrromethene difluoro (BODIPY) fluorophore in the alkyl side chain and methods of preparation. The compounds of the present invention can be used in fluorescence spectroscopy and fluorescence microscopy and in chromatography using fluorescence detection.
Labeling Of Target Molecules, Identification Of Organelles And Other Applications, Novel Compositions, Methods And Kits
The present invention provides dyes, reactive dyes and labeled reagents that may be used in the detection or quantification of desirable target molecules, such as proteins, nucleic acids and cellular organelles. Dyes are provided that may be used free in solution where the binding of the dye to the target molecule provides signal generation. Dyes are also provided that comprise reactive groups that may be used to attach the dyes to probes that will bind to desirable target molecules. The novel dyes of the present invention have been modified to provide beneficial properties.
Zaiguo Li - Little Neck NY, US Praveen Pande - Holbrook NY, US
Assignee:
Enzo Life Sciences, Inc. - Farmingdale NY
International Classification:
D06L 3/04
US Classification:
8648, 8622, 8633
Abstract:
The present invention provides a fluorescent dye that incorporates a pentavalent phosphorus or arsenic into its ring structure. Also provided is a fluorescence energy transfer system that comprises the above fluorescent dye and a second dye, where the second dye is capable of energy transfer with the fluorescent dye. Additionally provided is a kit for labeling a target molecule. In further embodiments, another kit for labeling a target molecule is provided. A target molecule labeled with the above-described fluorescent dye is also provided. In additional embodiments, methods of labeling a target molecule are provided.
Zaiguo Li - Little Neck NY, US Maciej Szczepanik - Mount Sinai NY, US Wayne Forrest Patton - Dix Hills NY, US
Assignee:
Enzo Life Sciences, Inc. - Farmingdale NY
International Classification:
C07D 417/02 C12Q 1/00
US Classification:
546172, 435 4, 435 6, 435 72, 548156
Abstract:
The present invention provides dyes, reactive dyes and labeled reagents that may be used in the detection or quantification of desirable target molecules, such as proteins, nucleic acids and cellular organelles. Dyes are provided that may be used free in solution where the binding of the dye to the target molecule provides signal generation. Dyes are also provided that comprise reactive groups that may be used to attach the dyes to probes that will bind to desirable target molecules. The novel dyes of the present invention have been modified to provide beneficial properties.
Free Cholesterol Analogs Bearing A Boron Dipyrromethene Difluoro (Bodipy) Fluorophore In The Side Chain And Method Of Preparation And Use Thereof
Robert Bittman - Roslyn Heights NY, US Zaiguo Li - Fresh Meadows NY, US
International Classification:
C07J 43/00
US Classification:
540 4
Abstract:
The present invention relates to free cholesterol analogs bearing a boron dipyrromethene difluoro (BODIPY) fluorophore in the side chain and methods of preparation. The compounds of the present invention can be used in fluorescence spectroscopy and fluorescence microscopy to visualize the exchange, distribution, and trafficking of free cholesterol between living cells and to monitor the movement of free cholesterol between ordered and disordered lipid domains in membranes.
Fluorochromes For Organelle Tracing And Multi-Color Imaging
Wayne Forrest Patton - Dix Hills NY, US Praveen Pande - Holbrook NY, US Yuejun Xiang - Bayside NY, US Zaiguo Li - Fresh Meadows NY, US James J. Donegan - Long Beach NY, US Elazar Rabbani - New York NY, US
International Classification:
C12Q 1/68 C07D 241/04 C07D 231/54 C07D 241/02
US Classification:
435 6, 544381, 5483565, 544357
Abstract:
Provided are compounds, methods and kits for identifying in cells of interest organelles including nuclei and a wide variety of organelles other than nuclei (non-nuclear organelles), as well as cell regions or cell domains. These compounds and methods can be used with other conventional detection reagents for identifying the location or position or quantity of organelles and even for distinguishing between organelles in cells of interest.
Wayne Forrest Patton - Dix Hills NY, US Jack Coleman - East Nothport NY, US Yuejun Xiang - Bayside NY, US Praveen Pande - Holbrook NY, US Zaiguo Li - Fresh Meadows NY, US
International Classification:
G01N 33/53 C12Q 1/02
US Classification:
435 724, 435 72, 435 29
Abstract:
Provided are assays useful for detecting and monitoring autophagy and phospholipidosis, including the progression of lysosomal storage diseases. Drugs and treatments for lysosomal storage diseases can be monitored for effectiveness in lysosomal storage disease conditions. Drug candidates and suspected toxic agents can also be screened for toxicity to cells, tissues and organs. Also provided are methods for distinguishing between phospholipidosis activators and autophagy pathway perturbation agents.
Provided are various compounds comprising the formulaAlso provided are fluorescent dyes comprising the above compound. Additionally, a fluorescence energy transfer system is provided that comprises the above-described fluorescent dye and a second dye, wherein the second dye is capable of energy transfer with the fluorescent dye. Further provided is a kit for labeling a target molecule, where the kit comprises the above-described fluorescent dye with additional reagents useful for labeling the target molecule. Additionally provided is a target molecule labeled with the above-described fluorescent dye. A method of labeling a target molecule is also provided. The method comprises contacting reactive group Z of the above-described fluorescent dye with the target molecule such that reactive group Z reacts with the target molecule to form a covalent bond between reactive group Z and the target molecule. Also, another method of labeling a target molecule is provided. The method comprises contacting the above-described fluorescent dye, which further comprises a member of a binding pair, with the target molecule, where the target molecule comprises a second member of the binding pair.