Zheng Q Wu

US Patent:

6689600, Feb 10, 2004

Filed:

Nov 16, 1999

Appl. No.:

09/441410

Inventors:

Zheng Wu - Sugar Land TX
Shuyuan Zhang - Media PA

Assignee:

Introgen Therapeutics, Inc. - Houston TX

International Classification:

C12N 104

US Classification:

4352351, 435239, 435243, 435260

Abstract:

The present invention addresses the need to improve the long-term storage stability (i. e. infectivity) of vector formulations. In particular, it has been demonstrated that for adenovirus, the use of bulking agents, cryoprotectants and lyoprotectants imparts desired properties that allow both lyophilized and liquid adenovirus formulations to be stored at 4Â C. for up to 6 months and retain an infectivity between 60-100% of the starting infectivity.

US Patent:

7125706, Oct 24, 2006

Filed:

Jun 12, 2001

Appl. No.:

09/880609

Inventors:

Shuyuan Zhang - Sugar Land TX, US
Capucine Thwin - Spring TX, US
Zheng Wu - Sugar Land TX, US
Toohyon Cho - Houston TX, US
Shawn Gallagher - Missouri City TX, US

Assignee:

Introgen Therapeutics, Inc. - Houston TX

International Classification:

C12N 7/01
C12N 15/861
A61K 39/235

US Classification:

4352351, 435239, 4353201, 4241991, 4242331, 424 932

Abstract:

The present invention addresses the need to improve the yields of viral vectors when grown in cell culture systems. In particular, it has been demonstrated that for adenovirus, the use of low-medium perfusion rates in an attached cell culture system provides for improved yields. In other embodiments, the inventors have shown that there is improved Ad-p53 production cells grown in serum-free conditions, and in particular in serum-free suspension culture. Also important to the increase of yields is the use of detergent lysis. Combination of these aspects of the invention permits purification of virus by a single chromatography step that results in purified virus of the same quality as preparations from double CsCl banding using an ultracentrifuge.

US Patent:

7235391, Jun 26, 2007

Filed:

Aug 28, 2001

Appl. No.:

09/941296

Inventors:

Zheng Wu - Sugar Land TX, US
Shuyuan Zhang - Media PA, US

Assignee:

Introgen Therapeutics, Inc. - Houston TX

International Classification:

C12N 1/04

US Classification:

4352351, 435239, 435243, 435260

Abstract:

The present invention addresses the need to improve the long-term storage stability (i. e. infectivity) of vector formulations. In particular, it has been demonstrated that for adenovirus, the use of bulking agents, cryoprotectants and lyoprotectants imparts desired properties that allow both lyophilized and liquid adenovirus formulations to be stored at 4 C. for up to 6 months and retain an infectivity between 60–100% of the starting infectivity.

US Patent:

7732129, Jun 8, 2010

Filed:

Dec 1, 1998

Appl. No.:

09/203078

Inventors:

Shuyuan Zhang - Sugar Land TX, US
Capucine Thwin - Spring TX, US
Zheng Wu - Sugar Land TX, US
Toohyon Cho - Houston TX, US
Shawn Gallagher - Missouri City TX, US

Assignee:

Crucell Holland B.V. - Leiden

International Classification:

C12Q 1/70

US Classification:

435 5

Abstract:

The present invention addresses the need to improve the yields of viral vectors when grown in cell culture systems. In particular, it has been demonstrated that for adenovirus, the use of low-medium perfusion rates in an attached cell culture system provides for improved yields. In other embodiments, the inventors have shown that there is improved Ad-p53 production with cells grown in serum-free conditions, and in particular in serum-free suspension culture. Also important to the increase of yields is the use of detergent lysis. Combination of these aspects of the invention permits purification of virus by a single chromatography step that results in purified virus of the same quality as preparations from double CsCl banding using an ultracentrifuge.

US Patent:

7888096, Feb 15, 2011

Filed:

Jun 26, 2007

Appl. No.:

11/768796

Inventors:

Zheng Wu - Sugar Land TX, US
Shuyuan Zhang - Media PA, US

Assignee:

Crucell Holland B.V. - Leiden

International Classification:

C12N 1/04
C12N 7/00

US Classification:

4352351, 435260, 4353201, 435239, 435243

Abstract:

The present invention addresses the need to improve the long-term storage stability (i. e. infectivity) of vector formulations. In particular, it has been demonstrated that for adenovirus, the use of bulking agents, cryoprotectants and lyoprotectants imparts desired properties that allow both lyophilized and liquid adenovirus formulations to be stored at 4 C. for up to 6 months and retain an infectivity between 60-100% of the starting infectivity.

US Patent:

7888097, Feb 15, 2011

Filed:

Oct 29, 2007

Appl. No.:

11/926854

Inventors:

Zheng Wu - Sugar Land TX, US
Shuyuan Zhang - Sugar Land TX, US

Assignee:

Crucell Holland B.V. - Leiden

International Classification:

C12N 7/00
C12N 1/04

US Classification:

4352351, 435260, 4353201, 4351723, 435239, 435243

Abstract:

The present invention addresses the need to improve the long-term storage stability (i. e. infectivity) of vector formulations. In particular, it has been demonstrated that for adenovirus, the use of bulking agents, cryoprotectants and lyoprotectants imparts desired properties that allow both lyophilized and liquid adenovirus formulations to be stored at 4 C. for up to 6 months and retain an infectivity between 60-100% of the starting infectivity.

US Patent:

20030008375, Jan 9, 2003

Filed:

Dec 27, 2001

Appl. No.:

10/033491

Inventors:

Shuyuan Zhang - Sugar Land TX, US
Capucine Thwin - Spring TX, US
Zheng Wu - Sugar Land TX, US
Toohyon Cho - Houston TX, US

Assignee:

Introgen Therapeutics, Inc.

International Classification:

C12N007/00
C12N007/02
C12N015/861

US Classification:

435/235100, 435/456000, 435/239000

Abstract:

The present invention addresses the need to improve the yields of viral vectors when grown in cell culture systems. In particular, it has been demonstrated that for adenovirus, the use of low-medium perfusion rates in an attached cell culture system provides for improved yields. In other embodiments, the inventors have shown that there is improved Ad-p53 production witrh cells grown in serum-free conditions, and in particular in serum-free suspension culture. Also important to the increase of yields is the use of detergent lysis. Combination of these aspects of the invention permits purification of virus by a single chromatography step that results in purified virus of the same quality as preparations from double CsCl banding using an ultracentrifuge.

US Patent:

20050175592, Aug 11, 2005

Filed:

Feb 18, 2005

Appl. No.:

11/062354

Inventors:

Zheng Wu - Sugar Land TX, US
Shuyuan Zhang - Media PA, US

International Classification:

C12N015/861
A61K048/00

US Classification:

424093200, 435456000

Abstract:

The present invention addresses the need to improve the long-term storage stability (i.e. infectivity) of vector formulations. In particular, it has been demonstrated that for adenovirus, the use of bulking agents, cryoprotectants and lyoprotectants imparts desired properties that allow both lyophilized and liquid adenovirus formulations to be stored at 4 C. for up to 6 months and retain an infectivity between 60-100% of the starting infectivity.

Address:

5514 Beechnut St, Houston, TX 77096

VIN:

4T1BE46K07U580620

Make:

TOYOTA

Model:

CAMRY

Year:

2007