Zhengyu Yu Feng

US Patent:

7371571, May 13, 2008

Filed:

Dec 17, 2003

Appl. No.:

10/738938

Inventors:

Raul G. Barletta - Lincoln NE, US
Zhengyu Feng - Austin TX, US

Assignee:

Board of Regents University of Nebraska - Lincoln - Lincoln NE

International Classification:

C12N 15/00
C12N 1/21
C12Q 1/00
C12P 21/06
C12N 9/00
C07K 14/00
C07H 21/04

US Classification:

4353201, 4352523, 4352531, 435 691, 435183, 435 4, 530350, 536 232

Abstract:

Recombinant mycobacterial strains which overproduce essential biosynthetic enzymes of pathogenic mycobateria are provided. These strains overproduce enzymes involved in the synthesis and incorporation of -alanine into mycobacterial peptidoglycan, the backbone of the mycobacterial cell wall. These overproducing strains may be used as reference strains in in vitro screening methods to identify antimycobacterial agents.

US Patent:

20060068469, Mar 30, 2006

Filed:

Aug 17, 2005

Appl. No.:

11/205883

Inventors:

Shelley Payne - Austin TX, US
Jaquelin Dudley - Austin TX, US
Stefan Seliger - Berkeley CA, US
Zhengyu Feng - Austin TX, US

Assignee:

Research Development Foundation - Carson City NV

International Classification:

C12P 21/06
C12N 9/00
C12N 1/21
C07K 14/54
C07K 14/53
C07K 14/52
C07K 14/475

US Classification:

435069100, 435252300, 435471000, 530350000, 435183000, 530351000, 530388100

Abstract:

The present invention provides bacterial vectors and fusion proteins containing a TTSS polypeptide, compositions of such fusion proteins including polynucleotides, and methods of delivering one or more genes into a target cell that involve contacting the cell with a composition that includes such a fusion protein. Compositions and methods of gene delivery that involve a bacterium and a TAT, Antp, or HSV VP22 polypeptide are also disclosed. The invention also concerns methods of delivering one or more genes into a target cell utilizing a bacterium capable of becoming internalized within the cell, wherein the bacterium includes one or more genes targeted for delivery to the cell, a gene encoding an RNA polymerase, and a gene that causes lysis of the bacterium.