Zhonggang Hou

US Patent:

20130236959, Sep 12, 2013

Filed:

Dec 17, 2012

Appl. No.:

13/717275

Inventors:

Guokai Chen - Rockville MD, US
James A. Thomson - Madison WI, US
Zhonggang Hou - Madison WI, US

International Classification:

C12N 5/074

US Classification:

435360, 530399, 536 235, 43525233, 435366

Abstract:

Thermostable FGF-2 proteins having enhanced ability to support human pluripotent stem cell cultures are provided. Also provided are methods and compositions utilizing thermostable FGF-2 proteins.

US Patent:

20120301962, Nov 29, 2012

Filed:

May 4, 2012

Appl. No.:

13/464235

Inventors:

James A. Thomson - Madison WI, US
Zhonggang Hou - Madison WI, US
Guokai Chen - Rockville MD, US

International Classification:

C12N 5/074
C12N 1/21
C12N 15/74
C07K 14/435
C12N 15/12

US Classification:

435377, 435395, 530395, 536 235, 4353201, 4352523

Abstract:

Vitronectin-derived cell culture substrates and methods of using the same for culturing pluripotent stem cells are presented.

US Patent:

20220315920, Oct 6, 2022

Filed:

Jun 21, 2019

Appl. No.:

17/254169

Inventors:

- Ithaca NY, US
- Ann Arbor MI, US
Yan ZHANG - Ann Arbor MI, US
Zhonggang HOU - Ann Arbor MI, US

International Classification:

C12N 15/11
C12N 9/22
C12N 15/90

Abstract:

Provided are compositions, methods, and kits for CRISPR-based editing of DNA targets by Type I CRISPR-associated (Cas) enzymes.

US Patent:

20190078054, Mar 14, 2019

Filed:

Sep 21, 2018

Appl. No.:

16/138724

Inventors:

- Madison WI, US
Nicholas E. Propson - Houston TX, US
Michael P. Schwartz - Madison WI, US
Zhonggang Hou - Madison WI, US
Gene I. Uenishi - Madison WI, US
Igor I. Slukvin - Verona WI, US
William L. Murphy - Waunakee WI, US
Jue Zhang - Madison WI, US

International Classification:

C12N 5/079
C12N 5/0786
G01N 33/50
C12N 5/071
C12N 5/0789
C12N 5/00

Abstract:

The present invention relates to methods for deriving human hematopoietic progenitors, primitive macrophages, and microglial cells from human pluripotent stem cells. In particular, provided herein are highly efficient and reproducible methods of obtaining human primitive macrophages and microglia from human pluripotent stem cells, where the primitive macrophages and microglia can be suitable for clinically relevant therapeutic applications.

US Patent:

20160186137, Jun 30, 2016

Filed:

Dec 31, 2015

Appl. No.:

14/986224

Inventors:

- Madison WI, US
Nicholas E. Propson - Houston TX, US
Michael P. Schwartz - Madison WI, US
Zhonggang Hou - Madison WI, US
Gene I. Uenishi - Madison WI, US
Igor I. Slukvin - Verona WI, US
William L. Murphy - Waunakee WI, US
Jue Zhang - Madison WI, US

International Classification:

C12N 5/079
C12N 5/071
G01N 33/50
C12N 5/0789

Abstract:

The present invention relates to methods for deriving human hematopoietic progenitors, primitive macrophages, and microglial cells from human pluripotent stem cells. In particular, provided herein are highly efficient and reproducible methods of obtaining human primitive macrophages and microglia from human pluripotent stem cells, where the primitive macrophages and microglia can be suitable for clinically relevant therapeutic applications.

US Patent:

20160186146, Jun 30, 2016

Filed:

Dec 31, 2015

Appl. No.:

14/986363

Inventors:

- Madison WI, US
William L. Murphy - Waunakee WI, US
Charles D. Page - Sun Prairie WI, US
Michael P. Schwartz - Madison WI, US
Zhonggang Hou - Madison WI, US

International Classification:

C12N 5/071

Abstract:

The present invention relates to three-dimensional (3D) tissue constructs and methods of using such 3D tissue constructs to screen for neurotoxic agents. In particular, provided herein are methods of producing and using complex, highly uniform human tissue models comprising physiologically relevant human cells, where the tissue models have the degree of sample uniformity and reproducibility required for use in quantitative high-throughput screening applications.

US Patent:

20150368610, Dec 24, 2015

Filed:

Aug 5, 2015

Appl. No.:

14/818779

Inventors:

- Madison WI, US
Zhonggang Hou - Madison WI, US
Guokai Chen - Madison WI, US

International Classification:

C12N 5/0735
C12N 5/074

Abstract:

Vitronectin-derived cell culture substrates and methods of using the same for culturing pluripotent stem cells are presented.

US Patent:

20140349405, Nov 27, 2014

Filed:

May 22, 2014

Appl. No.:

14/285252

Inventors:

- Evanston IL, US
- Madison WI, US
Alfonso Mondragon - Chicago IL, US
Rakhi Rajan - Skokie IL, US
James Thomson - Madison WI, US
Zhonggang Hou - Madison WI, US

Assignee:

WISCONSIN ALUMNI RESEARCH FOUNDATION - Madison WI
NORTHWESTERN UNIVERSITY - Evanston IL

International Classification:

C12N 15/85
C12N 9/22

US Classification:

435462, 435325, 435196, 536 232

Abstract:

Disclosed are components and methods for RNA-directed DNA cleavage and gene editing. The components include and the methods utilize a Cas9 protein from and one or more RNA molecules in order to direct the Cas9 protein to bind to and optionally cleave or nick a target sequence.