Armando Gabriel Sanchez

US Patent:

8189187, May 29, 2012

Filed:

Nov 13, 2009

Appl. No.:

12/617897

Inventors:

Marshall Donnie Graham - Nicholasville KY, US
William Gerry Graham - Nicholasville KY, US
James P. Clarkin - Scottsdale AZ, US
Mark A. Wells - Davie FL, US
Jose M. Cano - Miami FL, US
Carlos Alberto Arboleda - Miami FL, US
Armando Jose Sanchez - Miami FL, US

Assignee:

Beckman Coulter, Inc. - Brea CA

International Classification:

G01N 1/10

US Classification:

356246

Abstract:

An improved optical flow cell adapted for use in a flow cytometer for differentiating formed bodies (e. g. , blood cells). Manufactured from a monolithic transparent material, the improved flow cell has an internal flow channel of polygonal transverse cross-section through which prepared samples can be metered and an external envelope suited to acquisition of optical parameters from formed bodies in such samples. Preferably, such flow cell is formed by a glass-drawing process in which a relatively large glass preform having a rectilinear internal channel of a desired polygonal cross-sectional shape is heated and drawn to achieve a desired cross-sectional area of reduced size. Also disclosed are preferred methods for differentiating formed bodies using the flow cell of the invention.

US Patent:

20180188154, Jul 5, 2018

Filed:

Sep 25, 2017

Appl. No.:

15/714473

Inventors:

- Brea CA, US
William Gerry Graham - Nicholasville KY, US
James P. Clarkin - Scottsdale AZ, US
Mark A. Wells - Davie FL, US
Jose M. Cano - Miami FL, US
Carlos Alberto Arboleda - Miami FL, US
Armando J. Sanchez - Miami FL, US

Assignee:

Beckman Coulter, Inc. - Brea CA

International Classification:

G01N 21/05
C03B 23/047

Abstract:

An improved optical flow cell adapted for use in a flow cytometer for differentiating formed bodies (e.g., blood cells) in liquid suspensions. Preferably manufactured by assembling, aligning, and optically joining at least two elements made from transparent material, the improved flow cell has a seamless internal flow channel of preferably non-circular cross-section in a cylindrical first element through which prepared samples can be metered and an independent second element having an external envelope suited to acquisition of optical parameters from formed bodies in such suspensions, the second element being conforming and alignable to the first element so that non-axisymmetric refractive effects on optical characterizing parameters of formed bodies passing through the flow channel in the first element may be minimized before the two elements are optically joined and fixed in working spatial relationship. Preferably, such first element is formed by a glass-drawing process in which a relatively large glass preform having a rectilinear internal channel of a desired cross-sectional shape is heated and drawn to achieve a desired cross-sectional area of reduced size. Preferably, such second element is provided by through-boring a suitable commercial optical component to conform to such first element. Also disclosed are preferred methods for differentiating formed bodies using the flow cell of the invention.

US Patent:

20160011098, Jan 14, 2016

Filed:

Mar 14, 2014

Appl. No.:

14/770930

Inventors:

- Brea CA, US
William Gerry GRAHAM - Nicholasville KY, US
James P. CLARKIN - Scottsdale AZ, US
Mark A. WELLS - Davie FL, US
Jose M. CANO - Miami FL, US
Carlos Alberto ARBOLEDA - Miami FL, US
Armando J. SANCHEZ - Miami FL, US

International Classification:

G01N 21/05
C03C 27/00
G01N 15/14
C03B 23/047

Abstract:

An improved optical flow cell adapted for use in a flow cytometer for differentiating formed bodies (e.g., blood cells) in liquid suspensions. Preferably manufactured by assembling, aligning, and optically joining at least two elements made from transparent material, the improved flow cell has a seamless internal flow channel of preferably non-circular cross-section in a cylindrical first element through which prepared samples can be metered and an independent second element having an external envelope suited to acquisition of optical parameters from formed bodies in such suspensions, the second element being conforming and alignable to the first element so that non-axisymmetric refractive effects on optical characterizing parameters of formed bodies passing through the flow channel in the first element may be minimized before the two elements are optically joined and fixed in working spatial relationship.