Bin Jiang

US Patent:

20220330544, Oct 20, 2022

Filed:

Apr 15, 2022

Appl. No.:

17/659432

Inventors:

- College Park MD, US
Weijie Li - Shanghai, CN
Bin Jiang - College Park MD, US

International Classification:

A01N 1/02
C12N 5/074

Abstract:

Human induced pluripotent stem cells (hiPSCs) possess tremendous potential for tissue regeneration and banking hiPSCs by cryopreservation for their ready availability is crucial to their widespread use. However, contemporary methods for hiPSC cryopreservation are associated with both limited cell survival and high concentration of toxic cryoprotectants and/or serum. The latter may cause spontaneous differentiation and introduce xenogeneic factors, which may compromise the quality of hiPSCs. Here, sand from nature is discovered to be capable of seeding ice above −10 C., which enables cryopreservation of hiPSCs with no serum, minimized cryoprotectant, and high cell survival. Furthermore, the cryopreserved hiPSCs retain high pluripotency and functions judged by the pluripotency marker expression, cell cycle analysis, and capability of differentiation into the three germ layers. This unique sand-mediated cryopreservation method may greatly facilitate the convenient and ready availability of high-quality hiPSCs and probably many other types of cells/tissues for the emerging cell-based translational medicine.

US Patent:

20230035127, Feb 2, 2023

Filed:

Jul 21, 2022

Appl. No.:

17/814044

Inventors:

- College Park MD, US
Bin Jiang - College Park MD, US

International Classification:

G01N 33/50
C12N 5/077

Abstract:

Methods and compositions for the production of cardiomyocytes and cardiac organoids from the differentiation of pluripotent stem cells in three-dimensional (3D) culture are provided. Rock inhibitor, which has been ubiquitously used as a medium supplement to prevent apoptosis during handling and culture of pluripotent stem cells, compromises the capacity of cardiac differentiation of pluripotent stem cells in 3D culture at the most commonly used concentrations.