Deborah John Boles

US Patent:

8606529, Dec 10, 2013

Filed:

Mar 26, 2012

Appl. No.:

13/429911

Inventors:

Deborah Boles - Cary NC, US
Kenton C. Hasson - Germantown MD, US
Sami Kanderian - Germantown MD, US

Assignee:

Canon U.S. Life Sciences, Inc. - Rockville MD

International Classification:

G06F 19/00

US Classification:

702 19, 435 6, 435 71, 435 79, 435 911, 435 912, 544245, 544334, 544242, 544152, 536 231, 536 243

Abstract:

The application relates to methods and systems for analysis of dissociation behavior of nucleic acids and identification of nucleic acids. In one aspect, methods and systems are disclosed for identifying a nucleic acid in a sample including an unknown nucleic acid and for detecting a single nucleotide polymorphism in a nucleic acid in a sample. Methods and systems are also disclosed for identification of a nucleic acid in a biological sample including at least one unknown nucleic acid by fitting denaturation data including measurements of a quantifiable physical change of the sample at a plurality of independent sample property points to a function to determine an intrinsic physical value and to obtain an estimated physical change function, and identifying the nucleic acid in the biological sample by comparing the intrinsic physical value for at least one unknown nucleic acid to an intrinsic physical value for a known nucleic acid.

US Patent:

20060115830, Jun 1, 2006

Filed:

Jul 28, 2005

Appl. No.:

11/190942

Inventors:

Toru Takahashi - Tokyo, JP
Deborah Boles - Sterling VA, US

International Classification:

C12Q 1/68
C12P 19/34

US Classification:

435006000, 435091200

Abstract:

The invention provides novel SGP primers for improved use in waveform-profiling methods of DNA amplification. In one embodiment, use of an SGP primer in a method of DNA amplification results in exponential amplification of several distinct products. In another embodiment, the methods of the invention further comprise a novel half-time elongation step. In another embodiment, the distinct products may be detected via melting temperature analysis. The primers and methods of the invention may be combined to determine an organism in a sample.

US Patent:

20070026421, Feb 1, 2007

Filed:

Feb 9, 2006

Appl. No.:

11/352452

Inventors:

Steven Sundberg - San Francisco CA, US
Michael Knapp - Palo Alto CA, US
Ivor Knight - Arlington VA, US
Deborah Boles - Sterling VA, US
Aaron Rulison - Los Altos CA, US
Wesley Dong - Belmont CA, US
Edward Donlon - San Jose CA, US
Robert Moti - San Jose CA, US
Andrew Fabans - Los Gatos CA, US
Allen Boronkay - San Jose CA, US
Michael Slater - Modesto CA, US

Assignee:

Caliper Life Sciences, Inc. - Mountain View CA
Canon U.S. Life Sciences, Inc. - Rockville MD

International Classification:

C12Q 1/68

US Classification:

435006000

Abstract:

The present invention provides novel methods and devices that employ microfluidic technology to generate molecular melt curves. In particular, the devices and methods in accordance with the invention are useful in providing for the analysis of PCR amplification products.

US Patent:

20120088236, Apr 12, 2012

Filed:

Jun 2, 2011

Appl. No.:

13/152260

Inventors:

Jacquline Yancy - Laurel MD, US
Lingxia Jiang - Boyds MD, US
Stephanie Buszczak - Rockville MD, US
Renee Howell - Rockville MD, US
Deborah Boles - Cary NC, US

Assignee:

CANON U.S. LIFE SCIENCES, INC. - Rockville MD

International Classification:

C12Q 1/68

US Classification:

435 611

Abstract:

The present invention relates to methods and systems for genome scanning using high resolution melting analysis for identifying mutations and/or variants in genes of interest.

US Patent:

20120288865, Nov 15, 2012

Filed:

Apr 11, 2012

Appl. No.:

13/444502

Inventors:

Steven A. Sundberg - San Francisco CA, US
Michael R. Knapp - Palo Alto CA, US
Ivor T. Knight - Arlington VA, US
Deborah J. Boles - Sterling VA, US
Aaron J. Rulison - Los Altos CA, US
Wesley B. Dong - Belmont CA, US
Edward P. Donlon - San Jose CA, US
Robert J. Moti - San Jose CA, US
Andrew G. Fabans - Los Gatos CA, US
Allen R. Boronkay - San Jose CA, US
Michael Slater - Modesto CA, US

Assignee:

Canon U.S. Life Sciences, Inc. - Rockville MD
Caliper Life Sciences, Inc. - Mountain View CA

International Classification:

G01N 25/04

US Classification:

435 612

Abstract:

The present invention provides novel methods and devices that employ microfluidic technology to generate molecular melt curves. In particular, the devices and methods in accordance with the invention are useful in providing for the analysis of PCR amplification products.

US Patent:

20130178374, Jul 11, 2013

Filed:

Jul 6, 2012

Appl. No.:

13/542721

Inventors:

Allen E. Eckhardt - Durham NC, US
Jonathan Benton - Apex NC, US
Deborah Boles - Cary NC, US

Assignee:

ADVANCED LIQUID LOGIC, INC. - Research Triangle Park NC

International Classification:

C12N 15/10
C12Q 1/68

US Classification:

506 5, 506 38

Abstract:

The systems and methods of the invention provide a guided approach to pyrosequencing (i.e., hybrid pyrosequencing). A de novo nucleic acid sequence may compared to a library of possible results and the next nucleotide to be dispensed is selected based on the comparison of the de novo sequence and the library of possible results. In another example, at least the first nucleotide to be dispensed is selected based on a query of a database(s) of non-sequence parameters (e.g., incidence of infection, diagnostic symptoms, sample source) and subsequent dispensations determined based on a comparison of the de novo sequence and the library of possible results (e.g., candidate sequences). The systems and methods of the invention may be performed using a droplet actuator.

US Patent:

20180321170, Nov 8, 2018

Filed:

May 25, 2018

Appl. No.:

15/990369

Inventors:

- Rockville MD, US
Deborah John Boles - Cary NC, US
Aaron Rulison - Los Altos CA, US
Wesley B. Dong - Belmont CA, US
Andrew Fabans - Los Gatos CA, US
Allen Boronkay - San Jose CA, US
Edward Donlon - San Jose CA, US
Robert Moti - San Jose CA, US
Michael Slater - Modesto CA, US
Steven A. Sundberg - San Francisco CA, US
Michael R. Knapp - Palo Alto CA, US

Assignee:

Canon U.S. Life Sciences, Inc. - Rockville MD
CALIPER LIFE SCIENCES, INC. - MOUNTAIN VIEW CA

International Classification:

G01N 25/04
B01L 3/00
B01L 7/00
C12Q 1/6862
G01N 21/33
G01N 21/64
C12Q 1/6844
C12Q 1/686
C12Q 1/68

Abstract:

The present invention provides novel methods and devices that employ microfluidic technology to generate molecular melt curves. In particular, the devices and methods in accordance with the invention are useful in providing for the analysis of PCR amplification products.

US Patent:

20160377562, Dec 29, 2016

Filed:

Jun 27, 2016

Appl. No.:

15/194367

Inventors:

- Mountain View CA, US
- Rockville MD, US
Ivor T. Knight - Arlington VA, US
Deborah J. Boles - Sterling VA, US
Aaron Rulison - Los Altos CA, US
Wesley B. Dong - Belmont CA, US
Andrew Fabans - Los Gatos CA, US
Allen Boronkay - San Jose CA, US
Edwawrd Donlon - San Jose CA, US
Robert Moti - San Jose CA, US
Michael Slater - Modesto CA, US

Assignee:

CALIPER LIFE SCIENCES, INC. - MOUNTAIN VIEW CA
CANON U.S. LIFE SCIENCES, INC. - ROCKVILLE MD

International Classification:

G01N 25/04
G01N 21/33
B01L 3/00
G01N 21/64
C12Q 1/68
B01L 7/00

Abstract:

The present invention provides novel methods and devices that employ microfluidic technology to generate molecular melt curves. In particular, the devices and methods in accordance with the invention are useful in providing for the analysis of PCR amplification products.