Dominique Soldati

US Patent:

56437188, Jul 1, 1997

Filed:

Nov 4, 1993

Appl. No.:

8/147783

Inventors:

Kami Kim - San Francisco CA
Dominique Soldati - Mountain View CA
John C. Boothroyd - Stanford CA

Assignee:

The Board of Trustees of the Leland Stanford Junior University - Palo Alto CA

International Classification:

C12Q 168
C12N 111
C12N 1564
C12P 2102

US Classification:

435 6

Abstract:

A method of transient transfection of obligate intracellular parasites is described. This method is exploited to develop a system for stable transformation utilizing selectable genes. For example, introduction of the chloramphenicol acetyl transferase (CAT) gene fused to Toxoplasma flanking sequences followed by chloramphenicol selection results in parasites stably expressing CAT. DNA hybridization analysis indicated that the CAT gene had inserted via homologous recombination.