Douglas D Greiner from Duluth, MN, age 74

Us Patents

Sample Distribution Devices And Methods
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US Patent:

8337777, Dec 25, 2012
Filed:

Jun 25, 2007
Appl. No.:

11/768138
Inventors:

James C. Nurse - Pleasanton CA, US
Douglas P. Greiner - Fremont CA, US
Nigel P. Beard - Redwood City CA, US
Assignee:

Applied Biosystems, LLC - Carlsbad CA
International Classification:

B01L 3/00
US Classification:

422503, 422502, 422504, 422537, 137 1506, 137 67
Abstract:

A device and methods for sample distribution through a channel in which an expandable valve provides a mechanism to regulate flow through the channel. The valve may be configured to exert a force on a membrane layer so as to substantially block a portion of the channel to retain the sample in a desired location and prevent flow past the valve mechanism between the channel and a chamber.

Device And Method For Making Discrete Volumes Of A First Fluid In Contact With A Second Fluid, Which Are Immiscible With Each Other
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US Patent:

8361807, Jan 29, 2013
Filed:

Mar 22, 2011
Appl. No.:

13/069213
Inventors:

Willy Wiyatno - Union City CA, US
Linda Lee - Palo Alto CA, US
David Cox - Foster City CA, US
Richard Reel - Hayward CA, US
Mark Oldham - Emerald Hills CA, US
Jim Nurse - Pleasanton CA, US
Douglas Greiner - Fremont CA, US
Dennis Lehto - Santa Clara CA, US
Janusz Wojtowicz - Sunnyvale CA, US
Sam Woo - Redwood City CA, US
Assignee:

Applied Biosystems, LLC - Carlsbad CA
International Classification:

G01N 1/14
G01N 1/28
US Classification:

436180, 422 681, 13756501, 13756511, 7386422, 7386424, 7386434
Abstract:

Various embodiments described in the application relate to an apparatus, system, and method for generating, within a conduit, discrete volumes of one or more fluids that are immiscible with a second fluid. The discrete volumes can be used for biochemical or molecular biology procedures involving small volumes, for example, microliter-sized volumes, nanoliter-sized volumes, or smaller. The system can comprise an apparatus comprising at least one conduit operatively connected to one or more pumps for providing discrete volumes separated from one another by a fluid that is immiscible with the fluid(s) of the discrete volumes, for example, aqueous immiscible-fluid-discrete volumes separated by an oil.

Sample Presentation Device
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US Patent:

20050164402, Jul 28, 2005
Filed:

Jan 13, 2005
Appl. No.:

11/036707
Inventors:

Christopher Belisle - Concord CA, US
John Walker - Hayward CA, US
Sarah Ngola - Sunnyvale CA, US
Douglas Greiner - Fremont CA, US
Mark Levy - San Jose CA, US
Xiaoxia Zhao - Fremont CA, US
Irene Chen - Fremont CA, US
Mark Stolowitz - Pleasanton CA, US
Donald Paquin - San Jose CA, US
International Classification:

G01N001/00
US Classification:

436174000, 422099000
Abstract:

The present invention relates to sample presentation devices useful in performing analytical measurements. These devices have been configured to enable various aspects of liquid handling such as: retention, storage, transport, concentration, positioning, and transfer. Additionally, these devices can enhance the detection and characterization of analytes. The sample presentation devices of the present invention are comprised of one or more substrates having a plurality of zones of differing wettability. Methods of analyzing samples using the sample presentation device of the invention, as well as methods of making the sample presentation devices are disclosed.

Device And Method For Making Discrete Volumes Of A First Fluid In Contact With A Second Fluid, Which Are Immiscible With Each Other
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US Patent:

20070062583, Mar 22, 2007
Filed:

Aug 22, 2006
Appl. No.:

11/508044
Inventors:

David Cox - Foster City CA, US
Willy Wiyatno - Union City CA, US
Mark Oldham - Los Gatos CA, US
James Nurse - Pleasanton CA, US
Douglas Greiner - Fremont CA, US
Sam Woo - Redwood City CA, US
Richard Reel - Hayward CA, US
Dennis Lehto - Santa Clara CA, US
Linda Lee - Palo Alto CA, US
Janusz Wojtowicz - Sunnyvale CA, US
International Classification:

G01N 1/28
G01N 1/14
US Classification:

137565010, 436180000, 073864340, 137565110, 073864220, 073864240, 137625460, 137240000, 222420000
Abstract:

Various embodiments described in the application relate to an apparatus, system, and method for generating, within a conduit, discrete volumes of one or more fluids that are immiscible with a second fluid. The discrete volumes can be used for biochemical or molecular biology procedures involving small volumes, for example, microliter-sized volumes, nanoliter-sized volumes, or smaller. The system can comprise an apparatus comprising at least one conduit operatively connected to one or more pumps for providing discrete volumes separated from one another by a fluid that is immiscible with the fluid(s) of the discrete volumes, for example, aqueous immiscible-fluid-discrete volumes separated by an oil.

Sample Presentation Device
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US Patent:

20080248589, Oct 9, 2008
Filed:

May 19, 2005
Appl. No.:

11/596285
Inventors:

Christopher M. Belisle - Concord CA, US
John A. Walker - Hayward CA, US
Sarah M. Ngola - Sunnyvale CA, US
Douglas P. Greiner - Fremont CA, US
Mark J. Levy - San Jose CA, US
Xiaoxia Zhao - Fremont CA, US
Irene Y. Chen - Fremont CA, US
Mark L. Stolowitz - Pleasanton CA, US
Donald P. Paquin - San Jose CA, US
International Classification:

G01N 33/543
B01J 19/00
US Classification:

436518, 422 681
Abstract:

The present invention relates to sample presentation devices useful in performing analytical measurements. These devices have been configured to enable various aspects of liquid handling such as: retention, storage, transport, concentration, positioning, and transfer. Additionally, these devices can enhance the detection and characterization of analytes. The sample presentation devices of the present invention are comprised of one or more substrates having a plurality of zones of differing wettability. Methods of analyzing samples using the sample presentation device of the invention, as well as methods of making the sample presentation devices are disclosed.

Methods Of Modifying Support Surfaces For The Immobilization Of Particles And The Use Of The Immobilized Particles For Analyzing Nucleic Acids
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US Patent:

20090099027, Apr 16, 2009
Filed:

Aug 22, 2008
Appl. No.:

12/197132
Inventors:

Douglas P. GREINER - Fremont CA, US
Jeremy STUART - Charlotte NC, US
Meng C. TAING - Hayward CA, US
Steven M. MENCHEN - Fremont CA, US
Assignee:

APPLIED BIOSYSTEMS INC. - Foster City CA
International Classification:

C40B 20/04
C40B 50/18
C40B 40/04
US Classification:

506 4, 506 32, 506 15
Abstract:

Methods of modifying a nucleophilic surface of a support are described. The methods involve reacting a multifunctional electrophilic reagent with nucleophilic groups on the surface of the support. The resulting electrophilic surface can be used for the covalent attachment of particles (e.g. beads) having nucleophilic functional groups. For example, nucleic acid templates with nucleophilic (e.g., amine) groups can be attached to a surface of the particles. The nucleophilic groups on the nucleic acid templates can then be used to attach the particles to the modified surface of the support. The resulting support-bound particles can be used to analyze (e.g., sequence) the nucleic acid templates on the particles.

Methods And Systems For Nucleic Acid Sequencing Validation, Calibration And Normalization
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US Patent:

20100129810, May 27, 2010
Filed:

Sep 8, 2009
Appl. No.:

12/555549
Inventors:

Douglas P. GREINER - Fremont CA, US
Carmen GJERSTAD - Millbrae CA, US
Janet S. ZIEGLE - Berkeley CA, US
Lee W. JONES - Hayward CA, US
Min-Yi SHEN - Mountain View CA, US
Heinz BREU - Palo Alto CA, US
Assignee:

LIFE TECHNOLOGIES CORPORATION - Carlsbad CA
International Classification:

C12Q 1/68
US Classification:

435 6
Abstract:

A system for performing quality control for nucleic acid sample sequencing is disclosed. The system has a set of solid supports, each support having attached thereto a plurality of nucleic acid sequences. The set has plural groups of solid supports and each group contains solid supports having the same nucleic acid sequences attached thereto. The nucleic acid sequences of each group differ from each other. The nucleic acid sequences are synthetically derived. A method of preparing a quality control for performing nucleic acid sample sequencing and a method of validating a nucleic acid sequencing instrument are also disclosed.

Sample Presentation Device
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US Patent:

20110070659, Mar 24, 2011
Filed:

May 24, 2010
Appl. No.:

12/786133
Inventors:

Christopher M. Belisle - Concord CA, US
Sarah M. Ngola - Sunnyvale CA, US
Douglas P. Greiner - Fremont CA, US
Mark J. Levy - San Jose CA, US
Xiaoxia Zhao - Fremont CA, US
Irene Y. Chen - Fremont CA, US
Mark L. Stolowitz - Pleasanton CA, US
Donald P. Paquin - San Jose CA, US
Assignee:

Qiagen Sciences, Inc. - Hilde
International Classification:

G01N 33/543
G01N 1/00
G01N 33/50
B01L 9/00
G01N 21/00
G01N 21/76
G01N 24/00
G01N 30/96
US Classification:

436501, 436174, 436 86, 422566, 436164, 436172, 436173, 422 69
Abstract:

The present invention relates to sample presentation devices useful in performing analytical measurements. These devices have been configured to enable various aspects of liquid handling such as: retention, storage, transport, concentration, positioning, and transfer. Additionally, these devices can enhance the detection and characterization of analytes. The sample presentation devices of the present invention are comprised of one or more substrates having a plurality of zones of differing wettability. Methods of analyzing samples using the sample presentation device of the invention, as well as methods of making the sample presentation devices are disclosed.