Duc V Do

US Patent:

6613516, Sep 2, 2003

Filed:

Oct 11, 2000

Appl. No.:

09/689937

Inventors:

Fred C. Christians - Los Altos CA
Duc Do - San Jose CA
Thomas Gingeras - Encinitas CA
Kevin Gunderson - Encinitas CA
Charles G. Miyada - San Jose CA
Carsten Rosenow - Redwood City CA
Kai Wu - Mountain View CA
Qing Yang - San Jose CA

Assignee:

Affymetrix, Inc. - Santa Clara CA

International Classification:

C12Q 168

US Classification:

435 6, 435 71, 435 911, 435 912, 435 9132, 435194, 435196, 4352883, 436501, 536 231, 536 2433, 536 253

Abstract:

The presently claimed invention provides methods, compositions, and apparatus for studying nucleic acids. Specifically, the present invention provides a novel enrichment and labeling strategy for ribonucleic acids. In one embodiment, the invention provides enriching for a population of interest in a complex population by diminishing the presence of a target sequence. In a further embodiment, the invention can be used to reproducibly label and detect extremely small amounts of nucleic acids.

US Patent:

8388982, Mar 5, 2013

Filed:

Nov 19, 2009

Appl. No.:

12/622375

Inventors:

Xiangxu Kong - Foster City CA, US
Gene Shen - Santa Clara CA, US
Andrei Fedorov - San Mateo CA, US
John Lyle - Fremont CA, US
Grace Lee - San Ramon CA, US
Lubomir Sebo - Redwood City CA, US
Duc Do - San Jose CA, US
Robert Weber - Mountain View CA, US
Stephen Dudek - San Francisco CA, US

Assignee:

Pacific Biosciences of California, Inc. - Menlo Park CA

International Classification:

A61K 9/00
A61K 8/00
A61F 13/00
C12Q 1/68
C12Q 1/48
C12Q 1/02

US Classification:

424400, 424 59, 424422, 435 61, 435 15, 435 29

Abstract:

Compositions, devices, systems and methods for reducing and/or preventing photo-induced damage of one or more reactants in an illuminated analytical reaction by addition of one or more photo-induced damage mitigating agents to the reaction mixture and allowing the reaction to proceed for a period that is less than a photo-induced damage threshold period.

US Patent:

20010044105, Nov 22, 2001

Filed:

Dec 18, 2000

Appl. No.:

09/737761

Inventors:

Ali Laayoun - Lyon, FR
Duc Do - San Jose CA, US
Charles Miyada - San Jose CA, US

International Classification:

C12Q001/68
C12P019/34
C07H021/04

US Classification:

435/006000, 435/091100, 536/025320

Abstract:

A process is provided for labeling with signal amplification a ribonucleic acid (RNA), comprising fragmenting the RNA to form RNA fragments, fixing a first ligand to a terminal phosphate located at least one of the 3′ end and the 5′ end of each of a plurality of the RNA fragments, the terminal phosphate having been released during the fragmentation, and binding a plurality of labeling agents to the first ligand on each of a plurality of the fragments.

US Patent:

20050059009, Mar 17, 2005

Filed:

Jul 3, 2003

Appl. No.:

10/613365

Inventors:

Fred Christians - Los Altos CA, US
Duc Do - San Jose CA, US
Thomas Gingeras - Encinitas CA, US
Kevin Gunderson - Encinitas CA, US
Charles Miyada - San Jose CA, US
Carsten Rosenow - Redwood City CA, US
Kai Wu - Mountain View CA, US
Qing Yang - San Jose CA, US

Assignee:

Affymetrix, INC. - Santa Clara CA

International Classification:

C12Q001/68
C07H021/02
C07H021/04

US Classification:

435006000, 536025320

Abstract:

The presently claimed invention provides methods, compositions, and apparatus for studying nucleic acids. Specifically, the present invention provides a novel enrichment and labeling strategy for ribonucleic acids. In one embodiment, the invention provides enriching for a population of interest in a complex population by diminishing the presence of a target sequence. In a further embodiment, the invention can be used to reproducibly label and detect extremely small amounts of nucleic acids.

US Patent:

20070009939, Jan 11, 2007

Filed:

May 30, 2006

Appl. No.:

11/443879

Inventors:

Fred Christians - Los Altos Hills CA, US
Duc Do - San Jose CA, US
Thomas Gingeras - Encinitas CA, US
Kevin Gunderson - Encinitas CA, US
Charles Miyada - San Jose CA, US
Carsten Rosenow - Redwood City CA, US
Kai Wu - Mountain View CA, US
Qing Yang - San Jose CA, US

Assignee:

Affymetrix, INC. - Santa Clara CA

International Classification:

C12Q 1/68
C07H 21/02

US Classification:

435006000, 536025300

Abstract:

The presently claimed invention provides methods, compositions, and apparatus for studying nucleic acids Specifically, the present invention provides a novel enrichment and labeling strategy for ribonucleic acids In one embodiment, the invention provides enriching for a population of interest in a complex population by diminishing the presence of a target sequence In a further embodiment the invention can be used to reproducibly label and detect extremely small amounts of nucleic acids

US Patent:

20140057273, Feb 27, 2014

Filed:

Aug 22, 2013

Appl. No.:

13/973940

Inventors:

Austin P. So - Pleasanton CA, US
Duc Do - San Jose CA, US

International Classification:

G01N 21/64

US Classification:

435 612

Abstract:

Digital assay system, includes methods, apparatus, and compositions, for distinguishing and measuring different types of templates according to the different lengths of corresponding amplicons, which may be amplified by the same pair of primers. The system may include a length-sensitive reporter generating luminescence that varies according to amplicon length. The system may, for example, be utilized to identify, distinguish, and/or quantify wild-type and mutant/variant templates, processed and unprocessed template, a target template and a primer dimer, or the like.

US Patent:

20200385791, Dec 10, 2020

Filed:

Jun 8, 2020

Appl. No.:

16/896118

Inventors:

- Hercules CA, US
Zachary BURKETT - Walnut Creek CA, US
Man CHENG - Danville CA, US
Meiye WU - Tracy CA, US
Pranav PATEL - Fremont CA, US
Duc DO - San Jose CA, US
Arkadiusz BIBILLO - Walnut Creek CA, US

International Classification:

C12Q 1/6834
C12Q 1/6874
C12Q 1/6876
C12N 15/10

Abstract:

Provided are methods for detecting the presence of multiple barcoded solid supports in partitions, the method comprising providing a plurality of partitions wherein at least some partitions comprises multiple solid supports, where each solid support is linked to different solid support oligonucleotides, the solid support oligonucleotides comprising a barcode unique for the solid support; synthesizing a complementary sequence of the oligonucleotides to produce a double-stranded polynucleotide having barcodes from two of the solid support oligonucleotides; and sequencing one or both strands of the double-stranded polynucleotides, wherein sequences comprising two different barcodes indicates the presence of two solid supports in the same partition. Also provided are compositions and methods for producing the oligonucleotides.