Guobin C Luo

US Patent:

20100255487, Oct 7, 2010

Filed:

Mar 26, 2010

Appl. No.:

12/748168

Inventors:

JOSEPH BEECHEM - EUGENE OR, US
THEO NIKIFOROV - CARLSBAD CA, US
Vi-En Choong - Carlsbad CA, US
Xinzhan Peng - Carlsbad CA, US
Guobin Luo - Oceanside CA, US
Cheng-Yao Chen - Carlsbad CA, US
Michael Previte - Carlsbad CA, US

Assignee:

LIFE TECHNOLOGIES CORPORATION - CARLSBAD CA

International Classification:

C12Q 1/68
C12N 9/10

US Classification:

435 6, 435193

Abstract:

Provided herein are systems and methods for nucleotide incorporation reactions. The systems comprise polymerases having altered nucleotide incorporation kinetics and are linked to an energy transfer donor moiety, and nucleotide molecules linked with at least one energy transfer acceptor moiety. The donor and acceptor moieties undergo energy transfer when the polymerase and nucleotide are proximal to each other during nucleotide binding and/or nucleotide incorporation. As the donor and acceptor moieties undergo energy transfer, they generate an energy transfer signal which can be associated with nucleotide binding or incorporation. Detecting a time sequence of the generated signals, or the change in the signals, can be used to determine the order of the incorporated nucleotides, and can therefore be used to deduce the sequence of the target molecule.

US Patent:

20100330570, Dec 30, 2010

Filed:

May 28, 2010

Appl. No.:

12/790760

Inventors:

Peter B. VANDER HORN - Encinitas CA, US
Cheng-Yao Chen - Carlsbad CA, US
Guobin Luo - Oceanside CA, US
Michael Previte - Carlsbad CA, US
Jamshid Temirov - Germantown TN, US
Theo Nikiforov - Carlsbad CA, US
Zhaohui Zhou - San Ramon CA, US
Hongye Sun - Belmont CA, US
Yufang Wang - Foster City CA, US
Stefanie Yukiko Nishimura - Mountain View CA, US
Hongyi Wang - Pearland TX, US
Marian Peris - Belmont CA, US
Barnett B. Rosenblum - San Jose CA, US
Michael Phelan - Hayward CA, US

Assignee:

LIFE TECHNOLOGIES CORPORATION - Carlsbad CA

International Classification:

C12Q 1/68
C12N 9/96

US Classification:

435 6, 435188

Abstract:

Provided herein are compositions and systems for use in polymerase-dependent, nucleotide transient-binding methods. The methods are useful for deducing the sequence of a template nucleic acid molecule and single nucleotide polymorphism (SNP) analyses. The methods rely on the fact that the polymerase transient-binding time for a complementary nucleotide is longer compared to that of a non-complementary nucleotide. The labeled nucleotides transiently-binds the polymerase in a template-dependent manner, but does not incorporate. The methods are conducted under any reaction condition that permits transient binding of a complementary or non-complementary nucleotide to a polymerase, and inhibits nucleotide incorporation.

US Patent:

20120329042, Dec 27, 2012

Filed:

Jul 30, 2012

Appl. No.:

13/562159

Inventors:

Joseph BEECHEM - Eugene OR, US
Theo NIKIFOROV - Carlsbad CA, US
Vi-En CHOONG - Carlsbad CA, US
Xinzhan PENG - Carlsbad CA, US
Guobin LUO - Oceanside CA, US
Cheng-Yao CHEN - Carlsbad CA, US
Michael PREVITE - Carlsbad CA, US

Assignee:

LIFE TECHNOLOGIES CORPORATION - Carlsbad CA

International Classification:

G01N 21/64

US Classification:

435 61

Abstract:

Provided herein are systems and methods for nucleotide incorporation reactions. The systems comprise polymerases having altered nucleotide incorporation kinetics and are linked to an energy transfer donor moiety, and nucleotide molecules linked with at least one energy transfer acceptor moiety. The donor and acceptor moieties undergo energy transfer when the polymerase and nucleotide are proximal to each other during nucleotide binding and/or nucleotide incorporation. As the donor and acceptor moieties undergo energy transfer, they generate an energy transfer signal which can be associated with nucleotide binding or incorporation. Detecting a time sequence of the generated signals, or the change in the signals, can be used to determine the order of the incorporated nucleotides, and can therefore be used to deduce the sequence of the target molecule.

US Patent:

20130040365, Feb 14, 2013

Filed:

Aug 10, 2012

Appl. No.:

13/572488

Inventors:

Peter VANDER HORN - Encinitas CA, US
Theo NIKIFOROV - Carlsbad CA, US
Guobin LUO - Oceanside CA, US
Mindy LANDES - Carlsbad CA, US
Daniel MAZUR - San Diego CA, US
Eileen TOZER - San Diego CA, US
Tommie LINCECUM - Houston TX, US

Assignee:

LIFE TECHNOLOGIES CORPORATION - Carlsbad CA

International Classification:

C12N 9/12

US Classification:

435194

Abstract:

The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragment thereof are provided that allow for nucleic acid amplification. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates for use in various downstream processes. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered catalytic properties. In some aspects, the disclosure provides modified polymerases having enhanced catalytic properties as compared to a reference polymerase.

US Patent:

20130344561, Dec 26, 2013

Filed:

Feb 8, 2012

Appl. No.:

13/984346

Inventors:

John Davidson - Guilford CT, US
Theo Nikiforov - Carlsbad CA, US
Guobin Luo - Oceanside CA, US

Assignee:

LIFE TECHNOLOGIES CORPORATION - Carlsbad CA

International Classification:

C12N 11/10

US Classification:

435178

Abstract:

In some embodiments, the disclosure relates generally to methods as well as related compositions, systems, kits and apparatus comprising linking proteins to target compounds and/or to locations of interest using tethers. For example, the tether can used to link the protein to a target compound, for example, to link an enzyme to a substrate. Similarly, the tether can be used to link the protein at or near a desired location on a surface. In one group of embodiments, the tether includes a polynucleotide and the target compound or location on the surface includes another polynucleotide that is capable of hybridizing to the tether. In such bodiments, the tether can be used to link the protein to the target compound or location using nucleic acid hybridization.

US Patent:

20220315917, Oct 6, 2022

Filed:

May 10, 2021

Appl. No.:

17/302674

Inventors:

- Carlsbad CA, US
Daniel MAZUR - San Diego CA, US
Sihong CHEN - Vista CA, US
Guobin LUO - Oceanside CA, US
Xinzhan PENG - San Diego CA, US

International Classification:

C12N 15/10
C12Q 1/6806
C12Q 1/6853
C12Q 1/6855

Abstract:

Provided are methods for preparing a library of target nucleic acid sequences, as well as compositions and uses therefor. Methods comprise contacting a nucleic acid sample with a plurality of adaptors capable of amplification of one or more target nucleic acid sequences under conditions wherein the target nucleic acid(s) undergo a first amplification; digesting the resulting first amplification products; repairing the digested target amplicons; and amplifying the repaired products in a second amplification, thereby producing a library of target nucleic acid sequence. Each of the plurality of adaptor compositions comprise a handle and a targeted nucleic acid sequence and optionally one or more tag sequences. Provided methods may be carried out in a single, addition only workflow reaction, allowing for rapid production of highly multiplexed targeted libraries, optionally including unique tag sequences. Resulting library compositions are useful for a variety of applications, including sequencing applications.

US Patent:

20210284976, Sep 16, 2021

Filed:

May 7, 2021

Appl. No.:

17/302621

Inventors:

- Carlsbad CA, US
Peter B. VANDER HORN - Encinitas CA, US
Eileen TOZER - San Diego CA, US
Sihong CHEN - Vista CA, US
Guobin LUO - Oceanside CA, US
Joshua SHIRLEY - Carlsbad CA, US
Kevin HEINEMANN - Carlsbad CA, US

International Classification:

C12N 9/12
C12Q 1/686

Abstract:

The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, recombinant polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides recombinant polymerases that yield lower systematic error rates and/or improved accuracy, when used in sequencing by synthesis reactions as compared to a control polymerase. In one aspect, the disclosure relates to recombinant polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In another aspect, the recombinant polymerases are useful for the amplification of nucleic acid templates during PCR, emPCR, isothermal amplification, recombinase polymerase amplification, rolling circle amplification, strand displacement amplification and proximity ligation amplification. In some aspects, the disclosure relates to recombinant polymerases useful for the generation of nucleic acid libraries and/or nucleic acid templates.

US Patent:

20200277580, Sep 3, 2020

Filed:

Apr 16, 2020

Appl. No.:

16/850629

Inventors:

- Carlsbad CA, US
Theo NIKIFOROV - Carlsbad CA, US
Guobin LUO - Oceanside CA, US
Mindy LANDES - Carlsbad CA, US
Daniel MAZUR - San Diego CA, US
Eileen TOZER - San Diego CA, US
Tommie LINCECUM - Carlsbad CA, US

International Classification:

C12N 9/12
C12Q 1/6844
C12Q 1/686

Abstract:

The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragment thereof are provided that allow for nucleic acid amplification. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates for use in various downstream processes. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered catalytic properties. In some aspects, the disclosure provides modified polymerases having enhanced catalytic properties as compared to a reference polymerase.