Hoa Tuoi Thi Do

US Patent:

20200223913, Jul 16, 2020

Filed:

Jan 10, 2020

Appl. No.:

16/739724

Inventors:

- LAS VEGAS NV, US
Steven D. DAVIN - Seattle WA, US
Hoa Binh DO - Kirkland WA, US
Leon F. GARCIA-MARTINEZ - Woodinville WA, US
Geoffrey F. LEE - Mercer Island WA, US
Ethan W. OJALA - Snohomish WA, US
Mark YOUNG - Boulder CO, US
John A. LATHAM - Seattle WA, US

International Classification:

C07K 16/18
C07K 1/22
G01N 33/68
C07K 16/06
B01D 15/32
B01D 15/38
B01D 15/42
C07K 1/16
C07K 1/20
C07K 1/34
C07K 1/36

Abstract:

Processes for producing and purifying recombinant proteins are disclosed. In particular, the present disclosure provides processes of producing and purifying multi-subunit proteins expressed in yeast or filamentous fungal cells. The production and/or purification of such proteins are monitored for impurities, preferably using lectin binding assays, such that one or more process parameters may be adjusted to maximize the amount of desired recombinant protein and minimize the amount of glycosylated impurities. The processes can also be monitored for other undesired product-associated impurities, such as aggregates and nucleic acids. In exemplary embodiments, the recombinant proteins are multi-subunit proteins, such as antibodies, the host cell is a yeast, such as , and the glycosylated impurity is a glycovariant of the desired recombinant polypeptide, such as an N-linked and/or O-linked glycovariant.

US Patent:

20170137500, May 18, 2017

Filed:

Oct 21, 2016

Appl. No.:

15/331241

Inventors:

- LAS VEGAS NV, US
Steven D. Davin - Seattle WA, US
Hoa Binh Do - Kirkland WA, US
Leon F. Garcia-Martinez - Woodinville WA, US
Geoffrey F. Lee - Mercer Island WA, US
Ethan W. Ojala - Snohomish WA, US
Mark Young - Boulder CO, US
John A. Latham - Seattle WA, US

International Classification:

C07K 16/18
C07K 1/20
C07K 1/34
B01D 15/42
C07K 1/22
G01N 33/68
B01D 15/38
B01D 15/32
C07K 1/16
C07K 1/36

Abstract:

Processes for producing and purifying recombinant proteins are disclosed. In particular, the present disclosure provides processes of producing and purifying multi-subunit proteins expressed in yeast or filamentous fungal cells. The production and/or purification of such proteins are monitored for impurities, preferably using lectin binding assays, such that one or more process parameters may be adjusted to maximize the amount of desired recombinant protein and minimize the amount of glycosylated impurities. The processes can also be monitored for other undesired product-associated impurities, such as aggregates and nucleic acids. In exemplary embodiments, the recombinant proteins are multi-subunit proteins, such as antibodies, the host cell is a yeast, such as , and the glycosylated impurity is a glycovariant of the desired recombinant polypeptide, such as an N-linked and/or O-linked glycovariant.

US Patent:

20140288272, Sep 25, 2014

Filed:

Mar 17, 2014

Appl. No.:

14/215370

Inventors:

- Las Vegas NV, US
Steven D. DAVIN - Seattle WA, US
Hoa Binh DO - Kirkland WA, US
Leon F. GARCIA-MARTINEZ - Woodinville WA, US
Geoffrey F. LEE - Mercer Island WA, US
Ethan W. OJALA - Snohomish WA, US
Mark YOUNG - Boulder CO, US
John A. LATHAM - Seattle WA, US

Assignee:

ALDERBIO HOLDINGS LLC - Las Vegas NV

International Classification:

C07K 1/22
C07K 16/18

US Classification:

5303871, 530417, 435 691, 435 696

Abstract:

Processes for producing and purifying recombinant proteins are disclosed. In particular, the present disclosure provides processes of producing and purifying multi-subunit proteins expressed in yeast or filamentous fungal cells. The production and/or purification of such proteins are monitored for impurities, preferably using lectin binding assays, such that one or more process parameters may be adjusted to maximize the amount of desired recombinant protein and minimize the amount of glycosylated impurities. The processes can also be monitored for other undesired product-associated impurities, such as aggregates and nucleic acids. In exemplary embodiments, the recombinant proteins are multi-subunit proteins, such as antibodies, the host cell is a yeast, such as , and the glycosylated impurity is a glycovariant of the desired recombinant polypeptide, such as an N-linked and/or O-linked glycovariant.