Jonathan Renn

Jonathan Renn

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Jonathan Renn Chicago, IL

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Work:

Northwestern University, Department of Molecular Biosciences

Aug 2010 to 2000
Postdoctoral Fellow University of Notre Dame, Department of Chemistry and Biochemistry
Notre Dame, IN
Aug 2004 to Jun 2010
Graduate Student

Education:

University of Notre Dame
Notre Dame, IN
Jun 2010
Ph.D. in Biochemistry Benedictine University
Lisle, IL
May 2004
B.S. in Biochemistry

Skills:

Protein expression in vitro and in bacterial systems; protein refolding methods; FPLC and chromatography methods including affinity, ion exchange, size exclusion; affinity purification of proteasome; cell fractionation methods including bacterial outer membrane purification; Measuring protein folding and stability in vitro using fluorescence spectroscopy, circular dichroism spectroscopy and protease sensitivity methods; quantitative analysis of complex biological processes recapitulated in vitro; protein identification using mass spectrometry; Single-molecule force and distance measurements on proteins using magnetic tweezers; Site-directed mutagenesis; PCR; cloning; Quantification of protein secretion across the E. coli outer membrane; fluorescence microscopy; Western blotting; Familiar with a wide variety of chemistries used to functionalize beads and surfaces that bind proteins specifically; Adobe Photoshop and Illustrator; Microsoft Office Suite; SnapGene; KaleidaGraph; PyMOL; ImageJ; Endnote; Prism; BLAST; ExPASy