Kevin B Hoff

US Patent:

20130274129, Oct 17, 2013

Filed:

Apr 4, 2013

Appl. No.:

13/856978

Inventors:

- Regensburg, DE
Frank Notka - Regensburg, DE
Matthias Arnold - Regensburg, DE
Kevin Hoff - La Jolla CA, US
Stephen Whiteside - Frederick MD, US

International Classification:

C07K 14/195
C12N 15/66
C12N 15/10

US Classification:

506 9, 536 237, 530350, 536 231, 506 26, 506 14, 4353201, 435188, 43525233, 4352572, 435369

Abstract:

The invention generally relates to compositions and methods for designing and producing functional DNA binding effector molecules and associated customized services, tool kits and functional assays. In some aspects, the invention provides methods and tools for efficient assembly of customized TAL effector molecules. Furthermore, the invention relates to uses of TAL effector molecules and functional evaluation of such TAL by, for example, customized assays.

US Patent:

20220347192, Nov 3, 2022

Filed:

Sep 4, 2020

Appl. No.:

17/640196

Inventors:

- San Diego CA, US
Kevin G. Hoff - San Diego CA, US
Russell S. Komor - San Diego CA, US

International Classification:

A61K 31/60
C12N 1/20
C12N 15/52
C12P 7/42
C12P 17/06
A61K 31/05
A61K 31/352
A61K 35/742
A61K 35/74
A61K 36/062
A61K 36/02

Abstract:

Described herein are non-natural variants of prenyltronsfcrases having at least one amino acid substitution as compared to its corresponding natural or unmodified prenyltransferascs. The variants are capable of an increased rate of formation of prenylated aromatic compounds, such as cannabinoids, as compared to a wild type control The prcnyltransferase variants can be expressed in an engineered microbe having a pathway to such cannabinoids, and optionally can include one or more other pathway transgencs to promote formation of substrate(s) for the prcnyltransferases. Therapeutically useful cannabinoids can be purified from engineered cells and cell cultures.

US Patent:

20210277426, Sep 9, 2021

Filed:

Nov 19, 2020

Appl. No.:

16/953050

Inventors:

- San Diego CA, US
Nicholas Eakley - San Diego CA, US
Kevin Gregory Hoff - San Diego CA, US
Robin E. Osterhout - San Diego CA, US
Priti Pharkya - San Diego CA, US
Erik C. Ralph - San Diego CA, US
Stephen J. Van Dien - San Diego CA, US

International Classification:

C12P 5/02
C12N 9/04
C12N 9/02
C12P 7/16
C12P 7/04

Abstract:

Disclosed are methods and engineered microorganisms that enhance or improve the production of crotyl alcohol. The engineered microorganisms include genetic modifications in alcohol dehydrogenase, alkene reductase or both enzymatic activities. By such genetic modifications, a crotyl alcohol production pathway is provided or improved.

US Patent:

20210254030, Aug 19, 2021

Filed:

Mar 8, 2019

Appl. No.:

16/978629

Inventors:

- San Diego CA, US
Kevin G. Hoff - San Diego CA, US
Anna Lechner - San Diego CA, US
Harish Nagarajan - San Diego CA, US

International Classification:

C12N 9/10
C12P 7/42
C12P 7/22

Abstract:

Described herein are prenyltransferases including non-natural variants thereof having at least one amino acid substitution as compared to its corresponding natural or unmodified prenyltransferases and that are capable of at least two-fold greater rate of formation of cannabinoids such as cannabigerolic acid, cannabigerovarinic acid, cannabigerorcinic acid, and cannabigerol, as compared to a wild type control. Prenyltransferase variants also demonstrated regioselectivity to desired cannabinoid isomers such as CDBA (3-GOLA), 3-GDVA, 3-GOSA, and CBG (2-GOL). The prenyltransferase variants can be used to form prenylated aromatic compounds, and can be expressed in an engineered microbe having a pathway to such compounds, which include 3-GOLA, 3-GDVA, 3-GOSA, and CBG. 3-GOLA can be used for the preparation of cannabigerol (CBG), which can be used in therapeutic compositions.

US Patent:

20210047636, Feb 18, 2021

Filed:

Jun 29, 2020

Appl. No.:

16/915834

Inventors:

- Carlsbad CA, US
- Regensburg, DE
Frank NOTKA - Regenstauf, DE
Matthias ARNOLD - Regensburg, DE
Kevin HOFF - San Diego CA, US
Stephen WHITESIDE - Frederick MD, US

International Classification:

C12N 15/10
C07K 14/195
C12N 15/66
C12N 9/90
C12N 9/96

Abstract:

The invention generally relates to compositions and methods for designing and producing functional DNA binding effector molecules and associated customized services, tool kits and functional assays. In some aspects, the invention provides methods and tools for efficient assembly of customized TAL effector molecules. Furthermore, the invention relates to uses of TAL effector molecules and functional evaluation of such TAL by, for example, customized assays.

US Patent:

20190112596, Apr 18, 2019

Filed:

Apr 12, 2018

Appl. No.:

15/951938

Inventors:

- Carlsbad CA, US
Frank NOTKA - Regenstauf, DE
Matthias Arnold - Regensburg, DE
Kevin HOFF - San Diego CA, US
Stephen WHITESIDE - Frederick MD, US

International Classification:

C12N 15/10
C12N 9/96
C12N 9/90
C07K 14/195
C12N 15/66

Abstract:

The invention generally relates to compositions and methods for designing and producing functional DNA binding effector molecules and associated customized services, tool kits and functional assays. In some aspects, the invention provides methods and tools for efficient assembly of customized TAL effector molecules. Furthermore, the invention relates to uses of TAL effector molecules and functional evaluation of such TAL by, for example, customized assays.

US Patent:

20180135082, May 17, 2018

Filed:

Apr 7, 2016

Appl. No.:

15/564327

Inventors:

- San Diego CA, US
Nicholas Eakley - San Diego CA, US
Kevin Gregory Hoff - San Diego CA, US
Robin E. Osterhout - San Diego CA, US
Priti Pharkya - San Diego CA, US
Erik C. Ralph - San Diego CA, US
Stephen Van Dien - San Diego CA, US

Assignee:

GENOMATICA, iNC. - San Diego CA

International Classification:

C12P 5/02
C12P 7/16
C12N 9/04
C12N 9/02

Abstract:

Disclosed are methods and engineered microorganisms that enhance or improve the production of crotyl alcohol. The engineered microorganisms include genetic modifications in alcohol dehydrogenase, alkene reductase or both enzymatic activities. By such genetic modifications, a crotyl alcohol production pathway is provided or improved.

US Patent:

20160264962, Sep 15, 2016

Filed:

Jul 28, 2015

Appl. No.:

14/811363

Inventors:

- Carslbad CA, US
- Carlsbad CA, US
Frank Notka - Regenstauf, DE
Matthias Arnold - Regensburg, DE
Kevin Hoff - San Diego CA, US
Stephen Whiteside - Carlsbad CA, US

International Classification:

C12N 15/10
C12N 9/90
C07K 14/195
C12N 9/96

Abstract:

The invention generally relates to compositions and methods for designing and producing functional DNA binding effector molecules and associated customized services, tool kits and functional assays. In some aspects, the invention provides methods and tools for efficient assembly of customized TAL effector molecules. Furthermore, the invention relates to uses of TAL effector molecules and functional evaluation of such TAL by, for example, customized assays.