Kristopher M Zimmerman

US Patent:

8557970, Oct 15, 2013

Filed:

May 3, 2010

Appl. No.:

12/773002

Inventors:

Lance P. Encell - Fitchburg WI, US
Keith V. Wood - Mt. Horeb WI, US
Monika G. Wood - Mt. Horeb WI, US
Mary Hall - Waunakee WI, US
Paul Otto - Madison WI, US
Gediminas Vidugiris - Fitchburg WI, US
Kristopher Zimmerman - Madison WI, US

Assignee:

Promega Corporation - Madison WI

International Classification:

C12N 15/53
C12N 15/63
C12N 15/70
C12N 15/79
C12N 9/02

US Classification:

536 232, 435189, 4352523, 43525233, 4353201, 435810

Abstract:

A polynucleotide encoding a modified luciferase polypeptide. The modified luciferase polypeptide has at least 60% amino acid sequence identity to a wild-type luciferase and includes at least one amino acid substitution at a position corresponding to an amino acid in a wild-type luciferase of SEQ ID NO:1. The modified luciferase polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the wild-type luciferase.

US Patent:

20040101947, May 27, 2004

Filed:

Sep 26, 2003

Appl. No.:

10/673054

Inventors:

Laurie Engel - DeForest WI, US
John Shultz - Verona WI, US
Tonny Johnson - Madison WI, US
Kristopher Zimmerman - Madison WI, US
Laura Bozek - Madison WI, US
Judith Stevens - Oregon WI, US

Assignee:

Promega Corporation

International Classification:

C12N001/08

US Classification:

435/270000

Abstract:

Cell lysis compositions, methods for extracting and isolating proteins and peptides from a host cells using the compositions, kits and apparatus for extracting and isolating protein and peptide molecules from host cells and for detecting for the presence of a protein or peptide. The composition allows for the extraction and isolation of proteins and peptides from host cells without the need for mechanical disruption and with or without isolation of the cells from cell medium. The composition includes at least one surfactant having a hydrophobic-lipophilic balance value in the range from about 11 to about 16; and at least one cell membrane altering compound.

US Patent:

20070105154, May 10, 2007

Filed:

Oct 31, 2006

Appl. No.:

11/590252

Inventors:

Laurie Engel - DeForest WI, US
John Shultz - Verona WI, US
Tonny Johnson - Madison WI, US
Kristopher Zimmerman - Madison WI, US
Laura Bozek - Madison WI, US
Judith Stevens - Oregon WI, US

Assignee:

Promega Corporation - Madison WI

International Classification:

C40B 40/10
C40B 30/06
C12P 21/06

US Classification:

435007100, 435069100, 435320100, 435325000, 435068100, 435348000, 435419000, 435252300, 435254200

Abstract:

Cell lysis compositions, methods for extracting and isolating proteins and peptides from a host cells using the compositions, kits and apparatus for extracting and isolating protein and peptide molecules from host cells and for detecting for the presence of a protein or peptide. The composition allows for the extraction and isolation of proteins and peptides from host cells without the need for mechanical disruption and with or without isolation of the cells from cell medium. The composition includes at least one surfactant having a hydrophobic-lipophilic balance value in the range from about 11 to about 16; and at least one cell membrane altering compound.

US Patent:

20110283373, Nov 17, 2011

Filed:

May 11, 2011

Appl. No.:

13/105648

Inventors:

Brock BINKOWSKI - Sauk City WI, US
Braeden BUTLER - Madison WI, US
Lance P. ENCELL - Fitchburg WI, US
Frank FAN - Verona WI, US
Brad HOOK - Baraboo WI, US
Paul OTTO - Madison WI, US
Gediminas VIDUGIRIS - Fitchburg WI, US
Susan WIGDAL - Belleville WI, US
Kristopher ZIMMERMAN - Madison WI, US

International Classification:

A01K 67/00
C07H 21/00
C12N 1/00
C12N 15/63
C12N 5/10

US Classification:

800 13, 4353201, 435325, 435243, 435419, 536 232

Abstract:

A polynucleotide encoding a biosensor polypeptide comprising a modified circularly-permuted thermostable luciferase and a linker linking the C-terminal portion of the thermostable luciferase to the N-terminal portion of the thermostable luciferase. The modified circularly-permuted thermostable luciferase is modified relative to a parental circularly-permuted thermostable luciferase. The linker contains a sensor region capable of interacting with a target molecule in a cell. The modified circularly-permuted thermostable luciferase has an enhanced response after interaction of the biosensor with the target molecule relative to the parental circularly-permuted thermostable luciferase in the presence of the target molecule. Alternatively, the modified circularly-permuted thermostable luciferase has an enhanced response after interaction of the biosensor with the target molecule relative to the modified circularly-permuted thermostable luciferase in the absence of the target molecule.

US Patent:

20140030725, Jan 30, 2014

Filed:

Nov 12, 2012

Appl. No.:

13/674655

Inventors:

Braeden BUTLER - Madison WI, US
Lance P. ENCELL - Fitchburg WI, US
Frank FAN - Verona WI, US
Brad HOOK - Baraboo WI, US
Paul OTTO - Madison WI, US
Kimberly RISMA - Cincinnati OH, US
Gediminas VIDUGIRIS - Fitchburg WI, US
Susan WIGDAL - Belleville WI, US
Kristopher ZIMMERMAN - Madison WI, US

Assignee:

PROMEGA CORPORATION - Madison WI

International Classification:

C12Q 1/66
C12N 9/02

US Classification:

435 613, 536 232, 4353201, 435 8, 435 618, 435325

Abstract:

A polynucleotide encoding a biosensor polypeptide comprising a modified circularly-permuted thermostable luciferase and a linker linking the C-terminal portion of the thermostable luciferase to the N-terminal portion of the thermostable luciferase. The modified circularly-permuted thermostable luciferase is modified relative to a parental circularly-permuted thermostable luciferase. The linker contains a sensor region capable of interacting with a target molecule in a cell. The modified circularly-permuted thermostable luciferase has an enhanced response after interaction of the biosensor with the target molecule relative to the parental circularly-permuted thermostable luciferase in the presence of the target molecule. Alternatively, the modified circularly-permuted thermostable luciferase has an enhanced response after interaction of the biosensor with the target molecule relative to the modified circularly-permuted thermostable luciferase in the absence of the target molecule.

US Patent:

20220389434, Dec 8, 2022

Filed:

Jun 20, 2022

Appl. No.:

17/844371

Inventors:

- Madison WI, US
Mary Hall - Waunakee WI, US
Paul Otto - Madison WI, US
Gediminas Vidugiris - Fitchburg WI, US
Keith V. Wood - Mt. Horeb WI, US
Monika G. Wood - Mt. Horeb WI, US
Kristopher Zimmerman - Madison WI, US

International Classification:

C12N 15/52

Abstract:

A polynucleotide encoding a modified luciferase polypeptide. The modified luciferase polypeptide has at least 60% amino acid sequence identity to a wild-type luciferase and includes at least one amino acid substitution at a position corresponding to an amino acid in a wild-type luciferase of SEQ ID NO: 1. The modified luciferase polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the wild-type luciferase.

US Patent:

20210032676, Feb 4, 2021

Filed:

Oct 20, 2020

Appl. No.:

17/075415

Inventors:

- Madison WI, US
Mary Hall - Waunakee WI, US
Paul Otto - Madison WI, US
Gediminas Vidugiris - Fitchburg WI, US
Keith V. Wood - Mt. Horeb WI, US
Monika G. Wood - Mt. Horeb WI, US
Kristopher Zimmerman - Madison WI, US

International Classification:

C12Q 1/66
C12N 9/02

Abstract:

A polynucleotide encoding a modified luciferase polypeptide. The modified luciferase polypeptide has at least 60% amino acid sequence identity to a wild-type luciferase and includes at least one amino acid substitution at a position corresponding to an amino acid in a wild-type luciferase of SEQ ID NO:1. The modified luciferase polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the wild-type luciferase.

US Patent:

20200248228, Aug 6, 2020

Filed:

Mar 17, 2020

Appl. No.:

16/821682

Inventors:

- Madison WI, US
Mary Hall - Waunakee WI, US
Paul Otto - Madison WI, US
Gediminas Vidugiris - Fitchburg WI, US
Keith V. Wood - Mt. Horeb WI, US
Monika G. Wood - Mt. Horeb WI, US
Kristopher Zimmerman - Madison WI, US

International Classification:

C12Q 1/66
C12N 9/02

Abstract:

A polynucleotide encoding a modified luciferase polypeptide. The modified luciferase polypeptide has at least 60% amino acid sequence identity to a wild-type luciferase and includes at least one amino acid substitution at a position corresponding to an amino acid in a wild-type luciferase of SEQ ID NO:1. The modified luciferase polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the wild-type luciferase.