Li Q Jiang

US Patent:

7767454, Aug 3, 2010

Filed:

Nov 4, 2002

Appl. No.:

10/288555

Inventors:

John Joseph Harrington - Mentor OH, US
Paul David Jackson - Shaker Heights OH, US
Li Jiang - Hudson OH, US

Assignee:

ABT Holding Company - Cleveland OH

International Classification:

C12N 15/00

US Classification:

435455, 435440

Abstract:

The present invention is in the fields of molecular biology, cell biology, and genetics. The invention is directed generally to mutating genes in cells in vitro and in multi-cellular organisms. The invention encompasses methods for mutating genes in cells using polynucleotides that act as insertional mutagens. Such methods are used to achieve mutation of a single gene to achieve a desired phenotype as well as mutation of multiple genes, required cumulatively to achieve a desired phenotype, in a cell or in a multi-cellular organism. The invention is also directed to methods of identifying one or more mutated genes, made by the methods of the invention, in cells and in multi-cellular organisms, by means of a tagging property provided by the insertional mutagen(s). The insertional mutagen thus allows identification of one or more genes that are mutated by insertion of an insertional mutagen. The invention is also directed to methods for correlating a phenotype with a gene by screening or selecting cells that have been mutated by an insertional mutagen incorporated into one or more genes in a cell and identifying the gene or genes causing the phenotype by means of a tagging property in one or more of the insertional mutagens.

US Patent:

7972853, Jul 5, 2011

Filed:

Jan 15, 2003

Appl. No.:

10/345115

Inventors:

John Joseph Harrington - Mentor OH, US
Paul David Jackson - Shaker Heights OH, US
Li Jiang - Hudson OH, US

Assignee:

ABT Holding Company - Cleveland OH

International Classification:

C12N 15/01
C12N 15/00
C07H 21/04

US Classification:

435440, 435455, 435441, 435446, 536 231

Abstract:

The present invention is directed generally to reduction or inactivation of gene function or gene expression in cells in vitro and in multicellular organisms. The invention encompasses methods for mutating cells using a combination of mutagens, particularly wherein at least one mutagen is an insertional mutagen, to achieve homozygous gene mutation or mutation of multiple genes required cumulatively to achieve a phenotype to create knock-outs, knock-downs, and other modifications in the same cell. The invention is also directed to cells (and libraries thereof) and organisms created by the methods of the invention, including those in which at least one of the genes created by insertional mutagenesis is tagged by means of the insertion sequences thereby allowing identification of the mutated gene(s). The invention is also directed to libraries of mutated cells and their uses. The invention is also directed to methods of identifying mutations with methods of the invention, in cells (and libraries thereof) and organisms, by means of the insertional tag.

US Patent:

8153431, Apr 10, 2012

Filed:

Jan 15, 2003

Appl. No.:

10/342761

Inventors:

John Joseph Harrington - Mentor OH, US
Paul David Jackson - Shaker Heights OH, US
Li Jiang - Hudson OH, US

Assignee:

ABT Holding Company - Cleveland OH

International Classification:

C12N 15/01
C12N 15/00
C07H 21/04

US Classification:

435440, 435455, 435441, 435446, 536 231

Abstract:

The present invention is directed generally to reduction or inactivation of gene function or gene expression in cells in vitro and in multicellular organisms. The invention encompasses methods for mutating cells using a combination of mutagens, particularly wherein at least one mutagen is an insertional mutagen, to achieve homozygous gene mutation or mutation of multiple genes required cumulatively to achieve a phenotype to create knock-outs, knock-downs, and other modifications in the same cell. The invention is also directed to cells (and libraries thereof) and organisms created by the methods of the invention, including those in which at least one of the genes created by insertional mutagenesis is tagged by means of the insertion sequences thereby allowing identification of the mutated gene(s). The invention is also directed to libraries of mutated cells and their uses. The invention is also directed to methods of identifying mutations with methods of the invention, in cells (and libraries thereof) and organisms, by means of the insertional tag.

US Patent:

20040018624, Jan 29, 2004

Filed:

Oct 22, 2002

Appl. No.:

10/277612

Inventors:

John Harrington - Mentor OH, US
Paul Jackson - Shaker Heights OH, US
Li Jiang - Hudson OH, US

Assignee:

Athersys, Inc. - Cleveland OH

International Classification:

C12N015/85

US Classification:

435/455000

Abstract:

The present invention is directed generally to reduction or inactivation of gene function or gene expression in cells in vitro and in multicellular organisms. The invention encompasses methods for mutating cells using a combination of mutagens, particularly wherein at least one mutagen is an insertional mutagen, to achieve homozygous gene mutation or mutation of multiple genes required cumulatively to achieve a phenotype to create knock-outs, knock-downs, and other modifications in the same cell. The invention is also directed to cells (and libraries thereof) and organisms created by the methods of the invention, including those in which at least one of the genes created by insertional mutagenesis is tagged by means of the insertion sequences thereby allowing identification of the mutated gene(s). The invention is also directed to libraries of mutated cells and their uses. The invention is also directed to methods of identifying mutations with methods of the invention, in cells (and libraries thereof) and organisms, by means of the insertional tag.

US Patent:

20040253589, Dec 16, 2004

Filed:

Jan 15, 2003

Appl. No.:

10/342896

Inventors:

John Harrington - Mentor OH, US
Paul Jackson - Shaker Heights, US
Li Jiang - Hudson OH, US

Assignee:

Athersys, Inc. - Cleveland 44115

International Classification:

C12Q001/68
C12N015/85

US Classification:

435/006000, 435/455000

Abstract:

The present invention is directed generally to reduction or inactivation of gene function or gene expression in cells in vitro and in multiorganisms. The invention encompasses methods for mutating cells using a combination of mutagens, particularly wherein at least one mutagen is an insertional mutagen, to achieve homozygous gene mutation or mutation of multiple genes required cumulatively to achieve a phenotype to create s, s, and other modifications in the same cell. The invention is also directed to cells (and libraries thereof) and organisms created by the methods of the invention, including those in which at least one of the genes created by insertional mutagenesis is tagged by means of the insertion sequences thereby allowing identification of the mutated gene(s). The invention is also directed to libraries of mutated cells and their uses. The invention is also directed to methods of identifying mutations with methods of the invention, in cells (and libraries thereof) and organisms, by means of the insertional tag.

US Patent:

20040253590, Dec 16, 2004

Filed:

Jan 15, 2003

Appl. No.:

10/342923

Inventors:

John Harrington - Mentor OH, US
Paul Jackson - Shaker Heights OH, US
Li Jiang - Hudson OH, US

Assignee:

Athersys, Inc. - Cleveland 44115

International Classification:

C12Q001/68
C12N015/85

US Classification:

435/006000, 435/455000

Abstract:

The present invention is directed generally to reduction or inactivation of gene function or gene expression in cells in vitro and in multiorganisms. The invention encompasses methods for mutating cells using a combination of mutagens, particularly wherein at least one mutagen is an insertional mutagen, to achieve homozygous gene mutation or mutation of multiple genes required cumulatively to achieve a phenotype to create s, s, and other modifications in the same cell. The invention is also directed to cells (and libraries thereof) and organisms created by the methods of the invention, including those in which at least one of the genes created by insertional mutagenesis is tagged by means of the insertion sequences thereby allowing identification of the mutated gene(s). The invention is also directed to libraries of mutated cells and their uses. The invention is also directed to methods of identifying mutations with methods of the invention, in cells (and libraries thereof) and organisms, by means of the insertional tag.

US Patent:

20040253591, Dec 16, 2004

Filed:

Jan 15, 2003

Appl. No.:

10/342948

Inventors:

John Harrington - Mentor OH, US
Paul Jackson - Shaker Heights OH, US
Li Jiang - Hudson OH, US

Assignee:

Athersys, Inc. - Cleveland 44115

International Classification:

C12Q001/68
C12N015/85

US Classification:

435/006000, 435/455000

Abstract:

The present invention is directed generally to reduction or inactivation of gene function or gene expression in cells in vitro and in multiorganisms. The invention encompasses methods for mutating cells using a combination of mutagens, particularly wherein at least one mutagen is an insertional mutagen, to achieve homozygous gene mutation or mutation of multiple genes required cumulatively to achieve a phenotype to create s, s, and other modifications in the same cell. The invention is also directed to cells (and libraries thereof) and organisms created by the methods of the invention, including those in which at least one of the genes created by insertional mutagenesis is tagged by means of the insertion sequences thereby allowing identification of the mutated gene(s). The invention is also directed to libraries of mutated cells and their uses. The invention is also directed to methods of identifying mutations with methods of the invention, in cells (and libraries thereof) and organisms, by means of the insertional tag.

US Patent:

20100311612, Dec 9, 2010

Filed:

Jan 13, 2010

Appl. No.:

12/686470

Inventors:

John Joseph Harrington - Mentor OH, US
Paul David Jackson - Shaker Heights OH, US
Li Jiang - Hudson OH, US

Assignee:

ABT HOLDING COMPANY - Cleveland OH

International Classification:

C40B 40/02
C12N 15/01
C12Q 1/68
C12N 5/10

US Classification:

506 14, 435441, 435 6, 435446, 435447, 435448, 435325

Abstract:

The present invention is directed generally to reduction or inactivation of gene function or gene expression in cells in vitro and in multicellular organisms. The invention encompasses methods for mutating cells using a combination of mutagens, particularly wherein at least one mutagen is an insertional mutagen, to achieve homozygous gene mutation or mutation of multiple genes required cumulatively to achieve a phenotype to create knock-outs, knock-downs, and other modifications in the same cell. The invention is also directed to cells (and libraries thereof) and organisms created by the methods of the invention, including those in which at least one of the genes created by insertional mutagenesis is tagged by means of the insertion sequences thereby allowing identification of the mutated gene(s). The invention is also directed to libraries of mutated cells and their uses. The invention is also directed to methods of identifying mutations with methods of the invention, in cells (and libraries thereof) and organisms, by means of the insertional tag.