Ning Zhao

US Patent:

6410342, Jun 25, 2002

Filed:

Aug 19, 1997

Appl. No.:

08/914861

Inventors:

Rhett L. Affleck - Lawrenceville NJ
Doug Hobbs - Lansdale PA
Ilya Feygin - Mountainside NJ
Gregory L. Kirk - Skillman NJ
James A. Connelly - Falls Township PA
Ning Zhao - West Windsor NJ
James P. Mueller - Cranbury NJ
Peter Kieselbach - Upper Black Eddy PA

Assignee:

Pharmacopeia, Inc. - Princeton NJ

International Classification:

G01N 33543

US Classification:

436518, 530334, 530335, 435DIG 1, 435DIG 22, 435DIG 49

Abstract:

A method for photoeluting photocleavable linker-attached chemical compounds, and an apparatus suitable for implementing the method, are disclosed. According to the method, photocleavable linker-attached library compounds are photoeluted from solid support media under conditions of controlled light exposure and controlled temperature. An apparatus suitable for carrying out the present photoelution method includes means for substantially evenly illuminating the photocleavable linker, means for controlling illumination time and intensity, and means for controlling sample temperature.

US Patent:

6468787, Oct 22, 2002

Filed:

Apr 19, 1999

Appl. No.:

09/294169

Inventors:

Rhett L. Affleck - Lawrenceville NJ
Doug Hobbs - Lansdale PA
Ilya Feygin - Mountainside NJ
Gregory L. Kirk - Skillman NJ
James A. Connelly - Falls Township PA
Ning Zhao - West Windsor NJ
James P. Mueller - Cranbury NJ
Peter Kieselbach - Upper Black Eddy PA

Assignee:

Pharmacopeia, Inc. - Princeton NJ

International Classification:

C12M 100

US Classification:

4352921, 4352941, 435DIG 44, 422135, 4221863

Abstract:

A method for photoeluting photocleavable linker-attached chemical compounds, and an apparatus suitable for implementing the method, are disclosed. According to the method, photocleavable linker-attached library compounds are photoeluted from solid support media under conditions of controlled light exposure and controlled temperature. An apparatus suitable for carrying out the present photoelution method includes means for substantially evenly illuminating the photocleavable linker, means for controlling illumination time and intensity, and means for controlling sample temperature.

US Patent:

60902809, Jul 18, 2000

Filed:

Apr 19, 1999

Appl. No.:

9/294168

Inventors:

James A. Connelly - Falls Township PA
Linda O'Brien - Spotswood NJ
Sue Xiuqing Zhang - Somerset NJ
Ning Zhao - West Windsor NJ

Assignee:

Pharmacopeia, Inc. - Princeton NJ

International Classification:

B01D 1508

US Classification:

2101982

Abstract:

A method and apparatus for high-throughput identity confirmation, quantitation and purity determination of a compound of interest in a mixture of compounds is disclosed. According to the method, a chemical mixture, such as may be obtained from a chemical library, is processed to obtain a stream having a time-varying composition, such as by using a high pressure liquid chromatograph. The stream is split into two portions or streams for substantially contemporaneous detection in a compound-identifying device, such as a mass spectrograph, and in a compound-quantitating device, such as an evaporative light scattering detector. A peak in a chromatogram obtained from the compound-quantitating device is identified as the compound of interest by cross-correlation, via elution time, with chromatographic results obtained from the compound-identifying device. Once the peak is identified as corresponding to the compound of interest, the corresponding peak area is used for quantitation and purity determination.

US Patent:

59389320, Aug 17, 1999

Filed:

Nov 14, 1997

Appl. No.:

8/970941

Inventors:

James A. Connelly - Falls Township PA
Linda O'Brien - Spotswood NJ
Sue Xiuqing Zhang - Somerset NJ
Ning Zhao - West Windsor NJ

Assignee:

Pharmacopeia, Inc. - Princeton NJ

International Classification:

B01D 1508

US Classification:

210659

Abstract:

A method and apparatus for high-throughput identity confirmation, quantitation and purity determination of a compound of interest in a mixture of compounds is disclosed. According to the method, a chemical mixture, such as may be obtained from a chemical library, is processed to obtain a stream having a time-varying composition, such as by using a high pressure liquid chromatograph. The stream is split into two portions or streams for substantially contemporaneous detection in a compound-identifying device, such as a mass spectrograph, and in a compound-quantitating device, such as an evaporative light scattering detector. A peak in a chromatogram obtained from the compound-quantitating device is identified as the compound of interest by cross-correlation, via elution time, with chromatographic results obtained from the compound-identifying device. Once the peak is identified as corresponding to the compound of interest, the corresponding peak area is used for quantitation and purity determination.