Shi Zhan Huang

US Patent:

6660525, Dec 9, 2003

Filed:

Feb 10, 2000

Appl. No.:

09/501857

Inventors:

Francis J. Martin - San Francisco CA
Samuel Zalipsky - Redwood City CA
Shi Kun Huang - Castro Valley CA

Assignee:

Alza Corporation - Mountain View CA

International Classification:

C12N 1586

US Classification:

435458, 435375, 424450, 530402, 530403

Abstract:

A method of liposome-based therapy for a mammalian subject is disclosed. The method uses liposomes with outer surfaces that contain an affinity moiety effective to bind specifically to a biological surface at which the therapy is aimed, and a hydrophilic polymer coating effective to shield the affinity moiety from interaction with the target surface. The hydrophilic polymer coating is made up of polymer chains covalently linked to surface lipid components in the liposomes through releasable linkages. After a desired liposome biodistribution is achieved, a releasing agent is administered to cause cleaving of a substantial portion of the releasable linkages in the liposomes, to expose the affinity agent to the target surface.

US Patent:

6958241, Oct 25, 2005

Filed:

Jul 8, 2003

Appl. No.:

10/616094

Inventors:

Francis J. Martin - San Francisco CA, US
Samuel Zalipsky - Redwood City CA, US
Shi Kun Huang - Castro Valley CA, US

International Classification:

C12N015/88
C12N005/06
C12N005/12
A61K009/127

US Classification:

435458, 435375, 424450

Abstract:

A method of liposome-based therapy for a mammalian subject is disclosed. The method uses liposomes with outer surfaces that contain an affinity moiety effective to bind specifically to a biological surface at which the therapy is aimed, and a hydrophilic polymer coating effective to shield the affinity moiety from interaction with the target surface. The hydrophilic polymer coating is made up of polymer chains covalently linked to surface lipid components in the liposomes through releasable linkages. After a desired liposome biodistribution is achieved, a releasing agent is administered to cause cleaving of a substantial portion of the releasable linkages in the liposomes, to expose the affinity agent to the target surface.

US Patent:

6974589, Dec 13, 2005

Filed:

Oct 10, 2000

Appl. No.:

09/685940

Inventors:

Shi Kun Huang - Castro Valley CA, US
Samuel Zalipsky - Redwood City CA, US
Wei-Ming Zhang - San Francisco CA, US
Bei Jin - Union City CA, US
Yolanda P. Quinn - Daly City CA, US

Assignee:

Miza Corporation - Mountain View CA

International Classification:

A61K009/127

US Classification:

424450, 424 121, 424 9321, 424 951, 424417, 4284022

Abstract:

A lipid represented by the formula: wherein each of Rand Ris an alkyl or alkenyl chain having between about 8 to about 24 carbon atoms; n=0–20; L is selected from the group consisting of (i) —X—(C═O)—Y—, (ii) —X—(C═O)—, and (iii) —X—CH—, wherein X and Y are independently selected from oxygen, NH, and a direct bond; and Z is a weakly basic moiety that has a pK of less than about 7. 4 and greater than about 4. 0 is described.

US Patent:

7108863, Sep 19, 2006

Filed:

Mar 26, 2002

Appl. No.:

10/108154

Inventors:

Samuel Zalipsky - Redwood City CA, US
Theresa M. Allen - Edmonton, CA
Shi Kun Huang - Castro Valley CA, US

Assignee:

Alza Corporation - Mountain View CA

International Classification:

A61B 5/055
A61B 8/00
A61K 9/127

US Classification:

424450, 424 9321, 424 951

Abstract:

A liposomal composition and a method of using the same for achieving intracellular delivery of a liposome-entrapped agent is described. The liposomes are composed of a pH sensitive lipid and include a targeting ligand to direct the liposomes to a target cell. The liposomes also include a stabilizing component, such a polymer-derivatized lipid, where the polymer is attached to the lipid by a releasable linkage. Administration of the liposomes results in cellular internalization and destabilization of the liposome for intracellular delivery of the entrapped agent.

US Patent:

20050260261, Nov 24, 2005

Filed:

May 19, 2005

Appl. No.:

11/133879

Inventors:

Shi Huang - Castro Valley CA, US
Samuel Zalipsky - Redwood City CA, US
Wei-Ming Zhang - San Francisco CA, US
Bei Jin - Union City CA, US
Yolanda Quinn - Daly City CA, US

International Classification:

C07D233/54
A61K009/127

US Classification:

424450000, 548338100

Abstract:

A lipid represented by the formula: wherein each of Rand Ris an alkyl or alkenyl chain having between about 8 to about 24 carbon atoms; n=0-20; L is selected from the group consisting of (i) —X—(C═O)—Y—, (ii) —X—(C═O)—, and (iii) —X—CH—, wherein X and Y are independently selected from oxygen, NH and a direct bond; and Z is a weakly basic moiety that has a pK of less than about 7.4 and greater than about 4.0 is described.

US Patent:

20060240009, Oct 26, 2006

Filed:

Jun 20, 2006

Appl. No.:

11/472140

Inventors:

Samuel Zalipsky - Redwood City CA, US
Theresa Allen - Edmonton, CA
Shi Huang - Castro Valley CA, US

International Classification:

A61K 39/395
A61K 9/127

US Classification:

424144100, 424450000

Abstract:

A liposomal composition and a method of using the same for achieving intracellular delivery of a liposome-entrapped agent is described. The liposomes are composed of a pH sensitive lipid and include a targeting ligand to direct the liposomes to a target cell. The liposomes also include a stabilizing component, such a polymer-derivatized lipid, where the polymer is attached to the lipid by a releasable linkage. Administration of the liposomes results in cellular internalization and destabilization of the liposome for intracellular delivery of the entrapped agent.

US Patent:

58518185, Dec 22, 1998

Filed:

Mar 28, 1997

Appl. No.:

8/827236

Inventors:

Shi Kun Huang - Castro Valley CA
Edwin Kiyoshi Oto - Redwood City CA
Mohammad Hassanipour - Vallejo CA
Bei Jin - Union City CA

Assignee:

Sequus Pharmaceuticals, Inc. - Menlo Park CA

International Classification:

C12N 1564
A01N 2526
B01J 1302
C12P 2102

US Classification:

4353201

Abstract:

An improvement in a method of preparing plasmid-liposome complexes for in vivo transfection is described. The improvement includes selecting a condensing agent to condense the plasmid prior to contact with the liposomes, selecting a working medium and selecting a ratio of liposome lipid to plasmid. Also disclosed are DNA plasmid-liposome complexes formed by the method.

US Patent:

53566335, Oct 18, 1994

Filed:

Oct 7, 1992

Appl. No.:

7/958100

Inventors:

Martin C. Woodle - Menlo Park CA
Francis J. Martin - San Francisco CA
Shi K. Huang - Castro Valley CA

Assignee:

Liposome Technology, Inc. - Menlo Park CA

International Classification:

A61K 9127
A61K 3722

US Classification:

424450

Abstract:

A method of and composition for concentrating a therapeutic agent in an inflamed dermal region are disclosed. The composition, which is also used in the method, is a liposomal composition. The liposomes contain the therapeutic agent in entrapped form and are composed of vesicle-forming lipids including a vesicle-forming lipid derivatized with hydrophilic biocompatible polymer. After intravenous administration, the liposomes are taken up by the inflamed region within 24-48 hours, for site-specific release of entrapped compound into the inflamed region.