Shi H Huang

US Patent:

6468985, Oct 22, 2002

Filed:

Mar 17, 2000

Appl. No.:

09/528706

Inventors:

Shi Huang - San Diego CA

Assignee:

The Burnham Institute - La Jolla CA

International Classification:

A61K 3170

US Classification:

514 44, 4353201, 435455, 435456, 435458

Abstract:

The present invention provides mammalian retinoblastoma (Rb) protein-interacting zinc finger (RIZ) proteins, nucleic acid molecules encoding the RIZ and antibodies specific for the RIZ. The invention also provides screening assays for identifying an agent that effectively alters the association of a RIZ with a second molecule, which can bind to the RIZ. The invention also provides active fragments of RIZ containing the PR domain, which can regulate transcription. In addition, the invention provides methods for introducing a nucleic acid molecule-encoding a RIZ into a cell and for A-contacting a cell with an effective agent in order to modulate a function of a cell. Such methods are useful, for example, for inducing growth of a cardiac cell or a neuronal cell in a subject and for effecting normal growth control to a tumor cell or causing differentiation of tumor cells. The invention further provides methods for detecting a RIZ in a sample by detecting the RIZ or a nucleic acid molecule encoding the RIZ. Such methods are useful to diagnose a pathology that is characterized by an increased or decreased level of a RIZ in a cell or by expression of a mutant RIZ in a cell.

US Patent:

6586579, Jul 1, 2003

Filed:

Sep 3, 1999

Appl. No.:

09/389956

Inventors:

Shi Huang - San Diego CA

Assignee:

The Burnham Institute - La Jolla CA

International Classification:

C07H 2104

US Classification:

536 231, 536 235, 536 2433, 530350, 530324, 530327, 435 691, 4351723, 4353201, 435243, 435375, 435377, 435410, 435325, 435 5, 514 2, 514 12, 514 44

Abstract:

The invention provides an isolated PR Family Member (PFM) nucleic acid molecule that contains a PFM PR domain nucleotide sequence, a PFM ZF domain nucleotide sequence, or a modification thereof. The invention also provides an isolated PFM nucleic acid molecule that contains a nucleotide sequence that encodes a PFM PR domain polypeptide, or that encodes a PFM ZF domain polypeptide, or that encodes an immunologically equivalent modification thereof. Also provided are isolated PFM oligonucleotides. The invention also provides methods for detecting a PFM nucleic acid molecule in a sample. Further provided is a method of modulating cell growth by expressing an encoded PFM polypeptide in the cell.

US Patent:

6927030, Aug 9, 2005

Filed:

Dec 17, 2001

Appl. No.:

10/024450

Inventors:

Shi Huang - San Diego CA, US
Robert B. Chadwick - Castro Valley CA, US

Assignee:

The Burnham Institute - La Jolla CA
The Ohio State University - Columbus OH

International Classification:

C07H021/04
C12Q001/68

US Classification:

435 6, 435 911, 435 912, 536 231, 536 243

Abstract:

The invention provides a method of inhibiting growth of a microsatellite instability (MSI)-positive tumor. The method is practiced by introducing into an MSI-positive tumor a nucleic acid molecule encoding a RIZ1 polypeptide and expressing the RIZ1 polypeptide in the tumor in an effective amount to inhibit growth of the tumor. Also provided is a method of determining the MSI status of a tumor. The method is practiced by determining in the tumor the number of adenosine (A) nucleotides in a poly(A) tract of a RIZ nucleic acid molecule in the tumor. An abnormal number of adenosine nucleotides in a RIZ poly(A) tract indicates that the tumor is MSI-positive.

US Patent:

6955905, Oct 18, 2005

Filed:

Jul 18, 2002

Appl. No.:

10/200012

Inventors:

Shi Huang - San Diego CA, US

Assignee:

The Burnham Institute - La Jolla CA

International Classification:

C12N009/10
C12N015/00
C12N005/00
C12Q001/48
C07H021/04

US Classification:

435193, 435 15, 4353201, 435 691, 435325, 4352523, 536 232, 530350

Abstract:

The present invention provides an isolated nucleic acid molecule encoding a PFM/SET polypeptide. Also provided is an isolated nucleic acid molecule encoding a functional fragment of a PFM/SET polypeptide that contains a PR, SET, PRAZ or PKZL domain of a PFM/SET polypeptide of the invention. Further provided by the invention are PFM/SET polypeptides, and functional fragments thereof that contain a PR, SET, PRAZ or PKZL domain of a PFM/SET polypeptide. The invention also provides PFM/SET antibodies, PFM/SET modulatory compounds, and related methods. The molecules of the invention can be used in methods of screening for a compound that modulates PFM/SET polypeptide histone methyltransferase activity and to modulate cell proliferation to prevent or treat proliferative disorders, including cancer. Additionally, the molecules and methods of the invention can be used to diagnose and prognose proliferative disorders.

US Patent:

6989245, Jan 24, 2006

Filed:

May 9, 2002

Appl. No.:

10/142650

Inventors:

Shi Huang - San Diego CA, US

Assignee:

The Burnham Institute - La Jolla CA

International Classification:

C12Q 1/48
C12N 9/10
C07K 7/00

US Classification:

435 15, 435193, 530327

Abstract:

The invention provides a method of screening for a compound that modulates RIZ histone methyltransferase (HMT) activity, by contacting a RIZ or RIZ fragment having HMT activity with one or more candidate compounds, and determining histone methyltransferase activity of the contacted RIZ or RIZ fragment. Also provided is a method of screening for a compound that modulates progesterone receptor (PR) activity, by providing a RIZ1 modulatory compound, and determining the ability of the RIZ1 modulatory compound to modulate PR activity. Further provided is a method of identifying an individual with an estrogen receptor positive (ER+) tumor having a reduced likelihood of responding to endocrine therapy. The method involves determining the RIZ1 status of the tumor, wherein an abnormal RIZ1 status identifies the individual as an individual with a reduced likelihood of responding to endocrine therapy.

US Patent:

7335475, Feb 26, 2008

Filed:

May 3, 2005

Appl. No.:

11/121438

Inventors:

Shi Huang - San Diego CA, US

Assignee:

The Burnham Institute - La Jolla CA

International Classification:

C12Q 1/68
G01N 33/48
C12N 9/10
C12N 15/00
C12N 1/21
C12N 5/10
C12Q 1/48
C12P 21/06
C07H 21/04

US Classification:

435 6, 435193, 435 15, 4353201, 4352523, 435325, 435 691, 530350, 536 231, 536 232, 536 2433, 436 94

Abstract:

The present invention provides an isolated nucleic acid molecule encoding a PFM/SET polypeptide. Also provided is an isolated nucleic acid molecule encoding a functional fragment of a PFM/SET polypeptide that contains a PR, SET, PRAZ or PKZL domain of a PFM/SET polypeptide of the invention. Further provided by the invention are PFM/SET polypeptides, and functional fragments thereof that contain a PR, SET, PRAZ or PKZL domain of a PFM/SET polypeptide. The invention also provides PFM/SET antibodies, PFM/SET modulatory compounds, and related methods. The molecules of the invention can be used in methods of screening for a compound that modulates PFM/SET polypeptide histone methyltransferase activity and to modulate cell proliferation to prevent or treat proliferative disorders, including cancer. Additionally, the molecules and methods of the invention can be used to diagnose and prognose proliferative disorders.

US Patent:

7365158, Apr 29, 2008

Filed:

Feb 28, 2003

Appl. No.:

10/377079

Inventors:

Shi Huang - San Diego CA, US

Assignee:

The Burnham Institute - La Jolla CA

International Classification:

C07K 14/00
C07H 21/04
C12N 15/00
C12N 5/02

US Classification:

530350, 435 6, 435 691, 4353201, 435325, 536 235

Abstract:

The invention provides an isolated PR Family Member (PFM) nucleic acid molecule that contains a PFM PR domain nucleotide sequence, a PFM ZF domain nucleotide sequence, or a modification thereof. The invention also provides an isolated PFM nucleic acid molecule that contains a nucleotide sequence that encodes a PFM PR domain polypeptide, or that encodes a PFM ZF domain polypeptide, or that encodes an immunologically equivalent modification thereof. Also provided are isolated PFM oligonucleotides. The invention also provides methods for detecting a PFM nucleic acid molecule in a sample. Further provided is a method of modulating cell growth by expressing an encoded PFM polypeptide in the cell. Also provided is an isolated PFM polypeptide, containing a PFM PR domain amino acid sequence, or a PFM ZF domain amino acid sequence, or a modification thereof. The invention also provides an isolated PFM peptide, containing at least 8 contiguous amino acids of a PFM polypeptide.

US Patent:

20030049623, Mar 13, 2003

Filed:

Jul 18, 2001

Appl. No.:

09/910478

Inventors:

Shi Huang - San Diego CA, US

International Classification:

C12Q001/68
C07H021/04
C12P021/02
C12N005/06

US Classification:

435/006000, 435/455000, 435/325000, 435/320100, 435/069100, 530/350000, 536/023200

Abstract:

The present invention provides an isolated nucleic acid molecule encoding a PFM/SET polypeptide. Also provided is an isolated nucleic acid molecule encoding a functional fragment of a PFM/SET polypeptide that contains a PR, SET, PRAZ or PKZL domain of a PFM/SET polypeptide of the invention. Further provided by the invention are PFM/SET polypeptides, and functional fragments thereof that contain a PR, SET, PRAZ or PKZL domain of a PFM/SET polypeptide. The invention also provides PFM/SET antibodies, PFM/SET modulatory compounds, and related methods. The molecules and methods of the invention can be used to modulate cell proliferation to prevent or treat proliferative disorders, including cancer. Additionally, the molecules and methods of the invention can be used to diagnose and prognose proliferative disorders.