Wei S Liao

US Patent:

20100330706, Dec 30, 2010

Filed:

Jun 25, 2010

Appl. No.:

12/823988

Inventors:

Fang Wei - Santa Monica CA, US
Wei Liao - Santa Monica CA, US

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

G01N 33/545
B05D 5/12

US Classification:

436531, 427 211, 422 69

Abstract:

The present invention provides methods of making polymer-based biosensors and the biosensors made by said methods, wherein the biosensors comprise conducting polymers and negatively charged nanoparticles comprising a capture moiety. The present invention also provides methods of detecting analytes in a solution by contacting the solution with said polymer-based biosensors.

US Patent:

20190078159, Mar 14, 2019

Filed:

Apr 2, 2018

Appl. No.:

15/943298

Inventors:

- Oakland CA, US
Fang WEI - North Hills CA, US
Wei LIAO - North Hills CA, US

International Classification:

C12Q 1/6881
G01N 33/50
C12Q 1/6806
G01N 33/543
G01N 33/569
C12N 15/10
G01N 33/49

Abstract:

The molecules harbored in exosomes play important roles in biological science. A highly desirable goal for exosome research is the rapid, simple, simultaneous tracking and quantification of exosome harbored molecules. Disclosed herein are methods and devices for inducing the release and measurement of biomolecules harbored in exosomes. The disclosed method, Electric Field Induced Release and Measurement (EFIRM) technique, uses an electrical field to simultaneously disrupt exosomes to release the contents and measure the harbored exosomal RNA/proteins. The exosome vesicle contents can be released within minutes. This provides a potential on-site method for the detection of exosome-harbored biomolecules.

US Patent:

20190010538, Jan 10, 2019

Filed:

Jun 22, 2018

Appl. No.:

16/015407

Inventors:

- Dakland CA, US
Wei Liao - North Hills CA, US

International Classification:

C12Q 1/6806
G01N 33/53
G01N 1/40
C12Q 1/68
A61B 10/00

Abstract:

Provided herein is an all-in-one saliva collection apparatus that collects saliva to allow for the filtration of saliva in order to separate saliva components, such as extracellular proteins and nucleic acids that are not present in intact cells, from the intact cells and debris remaining in the extracted sample. The filtered saliva samples can be aliquoted into two fractions for protein and/or nucleic acid analysis. The present invention further describes long term storage at ambient temperatures of filtered salivary nucleic acids, and long term storage at ambient temperatures of filtered salivary proteins added to an ethanol solution. The filtered cell-free saliva samples have diagnostic usefulness.

US Patent:

20140329705, Nov 6, 2014

Filed:

Aug 6, 2012

Appl. No.:

14/236963

Inventors:

David T. Wong - Beverly Hills CA, US
Wei Liao - North Hills CA, US

Assignee:

THE REGENTS OF THE UNIVERSITY OF CALFORNIA - Oakland CA

International Classification:

C12Q 1/68
G01N 1/40
A61B 10/00
G01N 33/53

US Classification:

506 9, 435 612, 436501, 436 86, 435 792, 436176, 435 794, 435 5, 600573

Abstract:

Provided herein is an all-in-one saliva collection apparatus that collects saliva to allow for the filtration of saliva in order to separate saliva components, such as extracellular proteins and nucleic acids that are not present in intact cells, from the intact cells and debris remaining in the extracted sample. The filtered saliva samples can be aliquoted into two fractions for protein and/or nucleic acid analysis. The present invention further describes long term storage at ambient temperatures of filtered salivary nucleic acids, and long term storage at ambient temperatures of filtered salivary proteins added to an ethanol solution. The filtered cell-free saliva samples have diagnostic usefulness.

US Patent:

20140315195, Oct 23, 2014

Filed:

May 24, 2012

Appl. No.:

14/119843

Inventors:

David T. Wong - Beverly Hills CA, US
Fang Wei - North Hills CA, US
Wei Liao - North Hills CA, US

Assignee:

THE REGENTS OF THE UNIVERSITY OF CALIFORNIA - Oakland CA

International Classification:

C12Q 1/68
G01N 33/569

US Classification:

435 611, 435 721

Abstract:

The molecules harbored in exosomes play important roles in biological science. A highly desirable goal for exosome research is the rapid, simple, simultaneous tracking and quantification of exosome harbored molecules. Disclosed herein are methods and devices for inducing the release and measurement of biomolecules harbored in exosomes. The disclosed method, Electric Field Induced Release and Measurement (EFIRM) technique, uses an electrical field to simultaneously disrupt exosomes to release the contents and measure the harbored exosomal RNA/proteins. The exosome vesicle contents can be released within minutes. This provides a potential on-site method for the detection of exosome-harbored biomolecules.