Wei H Niu from Lincoln, NE, age 51

Us Patents

Microorganisms For The Production Of 1,4-Butanediol And Related Methods
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US Patent:

8129169, Mar 6, 2012
Filed:

Jun 4, 2010
Appl. No.:

12/794700
Inventors:

Stephen J. Van Dien - San Diego CA, US
Anthony P. Burgard - San Diego CA, US
Robert Haselbeck - San Diego CA, US
Catherine J. Pujol-Baxley - San Diego CA, US
Wei Niu - San Diego CA, US
John D. Trawick - San Diego CA, US
Harry Yim - San Diego CA, US
Mark J. Burk - San Diego CA, US
Robin E. Osterhout - San Diego CA, US
Jun Sun - San Diego CA, US
Assignee:

Genomatica, Inc. - San Diego CA
International Classification:

C12N 1/21
US Classification:

4352523
Abstract:

The invention provides non-naturally occurring microbial organisms comprising a 1,4-butanediol (BDO) pathway comprising at least one exogenous nucleic acid encoding a BDO pathway enzyme expressed in a sufficient amount to produce BDO and further optimized for expression of BDO. The invention additionally provides methods of using such microbial organisms to produce BDO.

Compositions And Methods For The Biosynthesis Of 1,4-Butanediol And Its Precursors
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US Patent:

8357520, Jan 22, 2013
Filed:

Jan 11, 2012
Appl. No.:

13/348564
Inventors:

Mark J. Burk - San Diego CA, US
Stephen J. Van Dien - San Diego CA, US
Anthony P. Burgard - Bellefonte PA, US
Wei Niu - San Diego CA, US
Assignee:

Genomatica, Inc. - San Diego CA
International Classification:

C12P 7/18
C12N 1/21
US Classification:

435158, 43525233
Abstract:

The invention provides a non-naturally occurring microbial biocatalyst including a microbial organism having a 4-hydroxybutanoic acid (4-HB) biosynthetic pathway having at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, or α-ketoglutarate decarboxylase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce monomeric 4-hydroxybutanoic acid (4-HB). Also provided is a non-naturally occurring microbial biocatalyst including a microbial organism having 4-hydroxybutanoic acid (4-HB) and 1,4-butanediol (BDO) biosynthetic pathways, the pathways include at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, 4-hydroxybutyrate:CoA transferase, 4-butyrate kinase, phosphotransbutyrylase, α-ketoglutarate decarboxylase, aldehyde dehydrogenase, alcohol dehydrogenase or an aldehyde/alcohol dehydrogenase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce 1,4-butanediol (BDO). Additionally provided are methods for the production of 4-HB and BDO.

Microorganisms For The Production Of 1,4-Butanediol, 4-Hydroxybutanal, 4-Hydroxybutyryl-Coa, Putrescine And Related Compounds, And Methods Related Thereto
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US Patent:

8377666, Feb 19, 2013
Filed:

Oct 13, 2010
Appl. No.:

12/904130
Inventors:

Robert Haselbeck - San Diego CA, US
John D. Trawick - La Mesa CA, US
Wei Niu - Lincoln NE, US
Anthony P. Burgard - Bellefonte PA, US
Assignee:

Genomatica, Inc. - San Diego CA
International Classification:

C12P 7/02
C12N 1/21
C12P 21/00
C12N 9/02
C12N 15/00
US Classification:

435155, 4352523, 435 691, 435189, 4353201
Abstract:

The invention provides non-naturally occurring microbial organisms comprising a 1,4-butanediol (BDO), 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine pathway comprising at least one exogenous nucleic acid encoding a BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine pathway enzyme expressed in a sufficient amount to produce BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine and further optimized for expression of BDO. The invention additionally provides methods of using such microbial organisms to produce BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine.

Microorganisms For The Production Of 1,4-Butanediol, 4-Hydroxybutanal, 4-Hydroxybutyryl-Coa, Putrescine And Related Compounds, And Methods Related Thereto
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US Patent:

8377667, Feb 19, 2013
Filed:

May 17, 2011
Appl. No.:

13/109732
Inventors:

Robert Haselbeck - San Diego CA, US
John D. Trawick - La Mesa CA, US
Wei Niu - Lincoln NE, US
Anthony P. Burgard - Bellefonte PA, US
Assignee:

Genomatica, Inc. - San Diego CA
International Classification:

C12P 7/02
C12N 1/21
C12P 21/00
C12N 15/00
C12N 9/02
US Classification:

435155, 4352523, 435 691, 435189, 4353201
Abstract:

The invention provides non-naturally occurring microbial organisms comprising a 1,4-butanediol (BDO), 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine pathway comprising at least one exogenous nucleic acid encoding a BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine pathway enzyme expressed in a sufficient amount to produce BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine and further optimized for expression of BDO. The invention additionally provides methods of using such microbial organisms to produce BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine.

Microorganisms And Methods For Carbon-Efficient Biosynthesis Of Mek And 2-Butanol
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US Patent:

8420375, Apr 16, 2013
Filed:

Jun 10, 2010
Appl. No.:

12/813469
Inventors:

Robin E. Osterhout - San Diego CA, US
Wei Niu - San Diego CA, US
Anthony P. Burgard - Bellefonte PA, US
Assignee:

Genomatica, Inc. - San Diego CA
International Classification:

C12N 1/20
C12N 1/00
C12N 9/00
C12P 7/26
C12P 7/16
C12P 21/06
C07H 21/04
US Classification:

4352523, 435243, 435148, 435160, 435183, 435 691
Abstract:

A non-naturally occurring microbial organism has at least one exogenous nucleic acid encoding a MEK pathway enzyme expressed in a sufficient amount to produce MEK. The MEK pathway includes an enzyme selected from an acetoacetyl-CoA dehydrogenase (bifunctional), an acetoacetyl-CoA aldehyde dehydrogenase, a 3-oxobutyraldehyde reductase, a 3-oxobutanol dehydratase, an MEK oxidoreductase, a 3-oxobutyraldehyde aminotransferase, a 4-aminobutan-2-one deaminase, a 2-amino-4-ketopentanoate (AKP) thiolase, an AKP aminotransferase, a 2,4-dioxopentanoate decarboxylase, an AKP deaminase, an acetylacrylate decarboxylase, an AKP decarboxylase, a glutamate dehydrogenase, a 3-oxobutyraldehyde oxidoreductase (aminating) and an AKP oxidoreductase (aminating). A 2-butanol pathway further includes an MEK reductase. A method for producing MEK or 2-butanol includes culturing these organisms under conditions and for a sufficient period of time to produce MEK or 2-butanol.

Microorganisms And Methods For The Coproduction 1,4-Butanediol And Gamma-Butyrolactone
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US Patent:

8530210, Sep 10, 2013
Filed:

Nov 4, 2010
Appl. No.:

12/940021
Inventors:

Jun Sun - San Diego CA, US
Mark J. Burk - San Diego CA, US
Anthony P. Burgard - Bellefonte PA, US
Robin E. Osterhout - San Diego CA, US
Wei Niu - Lincoln NE, US
John D. Trawick - La Mesa CA, US
Robert Haselbeck - San Diego CA, US
Assignee:

Genomatica, Inc. - San Diego CA
International Classification:

C12P 7/18
C12P 7/04
US Classification:

435158, 435126
Abstract:

The invention provides non-naturally occurring microbial organisms comprising 1,4-butanediol (14-BDO) and gamma-butyrolactone (GBL) pathways comprising at least one exogenous nucleic acid encoding a 14-BDO and GBL pathway enzyme expressed in a sufficient amount to produce 14-BDO and GBL. The invention additionally provides methods of using such microbial organisms to produce 14-BDO and GBL.

Compositions And Methods Of Producing Methionine
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US Patent:

8551742, Oct 8, 2013
Filed:

Apr 11, 2007
Appl. No.:

12/595817
Inventors:

Brian Brazeau - Oskaloosa IA, US
Jin-Sook Chang - Seoul, KR
Kwang Myung Cho - Gyeonggi-do, KR
Young Wook Cho - Seoul, KR
Mervyn Desouza - Plymouth MN, US
Holly J. Jessen - Chanhassen MN, US
So-Young Kim - Gyeonggi-do, KR
Wei Niu - Spring Park MN, US
Fernando A. Sanchez-Riera - Eden Prairie MN, US
Yong-Uk Shin - Gyeonggi-do, KR
Hyewon Um - Gyeonggi-do, KR
Assignee:

CJ Cheiljedang Corporation - Seoul
International Classification:

C12P 13/12
C12N 1/20
US Classification:

435113, 43525233
Abstract:

Described herein are microorganisms that produce methionine and related products from endogenous genes in a transsulfuration pathway, as well as from exogenous genes providing a direct sulfhydrylation pathway. Novel genes that are useful for methionine and SAMe production are disclosed.

Compositions And Methods For The Biosynthesis Of 1,4-Butanediol And Its Precursors
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US Patent:

20090075351, Mar 19, 2009
Filed:

Mar 14, 2008
Appl. No.:

12/049256
Inventors:

Mark J. Burk - San Diego CA, US
Stephen J. Van Dien - Encinitas CA, US
Anthony P. Burgard - Bellefonte PA, US
Wei Niu - San Diego CA, US
International Classification:

C12N 1/21
C12P 7/52
C12P 7/18
US Classification:

435141, 43525233, 435158
Abstract:

The invention provides a non-naturally occurring microbial biocatalyst including a microbial organism having a 4-hydroxybutanoic acid (4-HB) biosynthetic pathway having at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, or α-ketoglutarate decarboxylase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce monomeric 4-hydroxybutanoic acid (4-HB). Also provided is a non-naturally occurring microbial biocatalyst including a microbial organism having 4-hydroxybutanoic acid (4-HB) and 1,4-butanediol (BDO) biosynthetic pathways, the pathways include at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, 4-hydroxybutyrate:CoA transferase, 4-butyrate kinase, phosphotransbutyrylase, α-ketoglutarate decarboxylase, aldehyde dehydrogenase, alcohol dehydrogenase or an aldehyde/alcohol dehydrogenase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce 1,4-butanediol (BDO). Additionally provided is a method for the production of 4-HB. The method includes culturing a non-naturally occurring microbial organism having a 4-hydroxybutanoic acid (4-HB) biosynthetic pathway including at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase or α-ketoglutarate decarboxylase under substantially anaerobic conditions for a sufficient period of time to produce monomeric 4-hydroxybutanoic acid (4-HB). Further provided is a method for the production of BDO. The method includes culturing a non-naturally occurring microbial biocatalyst, comprising a microbial organism having 4-hydroxybutanoic acid (4-HB) and 1,4-butanediol (BDO) biosynthetic pathways, the pathways including at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, 4-hydroxybutyrate:CoA transferase, 4-hydroxybutyrate kinase, phosphotranshydroxybutyrylase, α-ketoglutarate decarboxylase, aldehyde dehydrogenase, alcohol dehydrogenase or an aldehyde/alcohol dehydrogenase for a sufficient period of time to produce 1,4-butanediol (BDO). The 4-HB and/or BDO products can be secreted into the culture medium.