The present invention concerns double stranded RNA compositions and transgenic plants capable of inhibiting expression of essential genes in parasitic nematodes, and methods associated therewith. Specifically, the invention relates to the use of RNA interference to inhibit expression of a target essential parasitic nematode gene, which is a parasitic nematode pas-5 gene, and relates to the generation of plants that have increased resistance to parasitic nematodes.
Aaron Wig - Chapel Hill NC, US Robert Ascenzi - Cary NC, US Sumita Chaudhuri - Cary NC, US Xiang Huang - Apex NC, US Rui-Guang Zhen - Chapel Hill NC, US Yu Han - Research Triangle Park NC, US
Assignee:
BASF Plant Science GmbH
International Classification:
A01H 5/00 A01H 1/00 C07H 21/04
US Classification:
800278, 800287, 800298, 536 241, 4353201, 435410
Abstract:
The invention provides plant gene promoters, and essential promoter elements, which are root-specific and/or induced by parasitic nematodes. The promoters of the invention are useful for controlling expression of nucleic acids of interest in plant roots.
Xiang Huang - Apex NC, US Ming Cheng - Cary NC, US Haiping Hong - Morrisville NC, US Yan Liu - Chapel Hill NC, US Kangfeng Mei - Apex NC, US
Assignee:
BASF Plant Sciences GmbH
International Classification:
C12N 15/82 C12N 5/04 A01H 5/00
US Classification:
435469, 435419, 800294, 8003172, 800312, 8003174
Abstract:
Disclosed herein are rooted plant assay systems, methods of making rooted plant assay systems, and methods of using rooted plant assay systems for high throughput screening of test polynucleotides for their ability to effect phenotypic traits having agricultural value.
Nematode Inducible Plant Mtn3-Like Gene Promotors And Regulatory Elements
Aaron Wiig - Chapel Hill NC, US Xiang Huang - Apex NC, US Sumita Chaudhuri - Cary NC, US
Assignee:
BASF Plant Science GmbH
International Classification:
C12N 15/63 C12N 15/82 A01H 1/00
US Classification:
800287, 800278, 536 241
Abstract:
The invention provides promoter polynucleotides that are root-preferred and/or induced by parasitic nematodes. The promoter polynucleotides of the invention are useful for controlling expression of nucleic acids of interest in plant roots.
Pathogen Inducible Plant Thehalose-6-Phophate Phophatase Gene Promoters And Regulatory Elements
Aaron Wiig - Chapel Hill NC, US Robert Ascenzi - Cary NC, US Xiang Huang - Apex NC, US Sumita Chaudhuri - Cary NC, US Rui-Guang Zhen - Chapel Hill NC, US Yu Han - Cary NC, US
Assignee:
BASF Plant Science GmbH
International Classification:
C12N 15/82 C12N 15/63 C12N 15/87
US Classification:
800287, 4353201, 536 241
Abstract:
The invention provides plant gene promoters and regulatory elements that are root-specific and/or induced by parasitic nematodes. The promoters of the invention are useful for controlling expression of nucleic acids of interest in plants' roots. The invention also provides expression cassettes including the plant gene promoters and regulatory elements of the invention, transgenic plants containing such expression cassettes, and methods of producing such transgenic plants.
Aaron Wiig - Chapel Hill NC, US Robert Ascenzi - Cary NC, US Xiang Huang - Apex NC, US Sumita Chaudhuri - Cary NC, US Rui-Guang Zhen - Chapel Hill NC, US Yu Han - Cary NC, US
Assignee:
BASF Plant Science GmbH
International Classification:
C12N 15/82 C12N 15/87 C12N 15/63
US Classification:
800287, 536 241, 4353201
Abstract:
The invention provides isolated promoter polynucleotides that are root-specific and/or induced by plant parasitic nematodes. The promoters of the invention are useful for controlling expression of nucleic acids of interest in plant roots and are particularly useful for controlling transcription of nucleic acids encoding agents that disrupt formation or maintenance of parasitic nematode feeding sites in plants.
Compositions And Methods Using Rna Interference For Control Of Nematodes
Peifeng REN - Cary NC, US Xiang HUANG - Apex NC, US Sumita CHAUDHURI - Cary NC, US Lawrence TALTON - Sanford NC, US John MCMILLAN - Raleigh NC, US
Assignee:
BASF PLANT SCIENCE GMBH - Ludwigshafen
International Classification:
A01H001/00 C07H021/04 C12N015/82 C12N005/04
US Classification:
800279000, 435419000, 435468000, 536023200
Abstract:
The present invention concerns double stranded RNA compositions and transgenic plants capable of inhibiting expression of essential genes in parasitic nematodes, and methods associated therewith. Specifically, the invention relates to the use of RNA interference to inhibit expression of a target essential parasitic nematode gene selected from the group consisting of a parasitic nematode cytosolic chaperonin gene, a parasitic nematode gene encoding heat shock protein-90, a parasitic nematode gene homologous to the Y65B4BR.5a gene, and a parasitic nematode gene homologous to a pat-10 gene, and relates to the generation of plants that have increased tolerance to parasitic nematodes.
The invention provides an improvement in the method of screening roots, both transgenic and non-transgenic, for activity against parasitic nematodes. In particular, the improvement of the invention is use of multi-well cell culture plates for root culture and nematode infection steps.
Novozymes Aug 1, 2014 - Sep 2019
Senior Department Manager at Novozymes
Weichert, Realtors Mark Thomas Properties Aug 1, 2014 - Sep 2019
Realtor and Broker
Syngenta Aug 2007 - Aug 2014
Team Leader and Senior Research Scientist Ii
Basf Jan 2002 - Aug 2007
Research Scientist
Education:
University of California, Riverside 1996 - 2001
Doctorates, Doctor of Philosophy
Skills:
Molecular Biology Biotechnology Life Sciences Genetics Genomics Assay Development Microbiology Dna R&D Molecular Genetics Pcr Technology Transfer Bioinformatics Agriculture Lifesciences Plant Pathology Polymerase Chain Reaction Protein Chemistry Research and Development Tissue Culture
Interests:
Science and Technology Children Arts and Culture Environment