Skills:
                                    EXPERIMENTAL SKILLS Surgical Skills/models: Coronary artery ischemia surgeries (ischemia/reperfusion, permanent occlusion, ischemic preconditioning) in rodents Cardiac pacing in rats Hindlimb ischemia in rodents Intramyocardial injection with adenovirus Carotid artery, jugular vein, femoral artery catheterization in rodents, rabbits, and canines Assistant in large animal surgeries, non-human primates, swines, canines, rabbits, and rats (intramyocardial gene delivery, instrumentation, such as Konigsberg (for LV, RV pressure), vascular catheterization (for intracoronary drug delivery, aortic and pulmonary arterial pressure), flow probe (for coronary artery, renal, pulmonary artery flow), Ultrasonic crystals (for LV segmental length, wall thickness)) Conscious animal coronary artery occlusion (swines, rabbits) and stenosis (swines) Flow probe implantation in rodents (cardiac output, femoral artery flow) Telemetry implantation (ECG, pressure) in rodents Mini osmotic pump implantation In vivo studies: Chronic drug delivery in rodents and canines Neutral and radioactive microsphere study for regional blood flow (swines, canines, rodents) Hemodynamic study on Non-human primates and rodents on tether system (including put animals on/off tether) Conscious animal (swines, canines, rabbits, rats) hemodynamic study (drug dose response) Mornitoring oxygen consumption in metabolic chamber and treadmill system Stem cell therapy in cardiac ischemia reperfusion Infarct size study 1) Aortic cannulation for LV perfusion with alcian blue or evans blue 2) Aortic, coronary artery cannulation for dual perfusion in swines 3) TTC staining for infarct size assessment 4) measure infarct size on both acute and chronic ischemic models) Diabetic/obese study 1) Glucose tolerance test 2) Insulin sensitivity test Blood and tissue sampling, such as brain, cardiovascular system, fat pads and all other major organs. Proficient with notocord, powerlab Ex vivo Techniques: Langendorf perfusion system: Measurement of mice, rats oxygen consumption, hemodynamic variables In vitro Techniques: Mammalian cell culture, isolation of neonatal and adult rat cardiomyocytes and vascular smooth muscle cells DNA and RNA extraction Primer design for PCR and qPCR Protein analysis by SDS-PAGE and Western Blot electrophoresis ELISA Cell transfection Histological staining and quantitating for angiogenesis and myocardial remodeling (H&E, tricrome, WGA, TUNEL, PASR, immunoflorescent staining, immunochemistry staining, etc.) Company contract study Experience Asubio, Japan Gene therapy in swines with permanent Left circumflex coronary artery occlusion Cytokinetics Drug testing in canines model of myocardial infarction induced heart failure Drug testing in canine model of hypertension with/without angiotensin II Evaluation of novel compounds for the treatment of pulmonary hypertension utilizing swine model of pulmonary hypertension Genzyme Chronic drug delivery/treatment in rodent model of cardiac ischemia reperfusion injury GSK Drug testing (chronic injection) in rodents with ischemia reperfusion Drug testing (chronic gavage) in rodents with thoracic aortic constriction Amgen Drug testing in rodents with myocardial infarction induced heart failure