Ying Wu Huang

US Patent:

6641708, Nov 4, 2003

Filed:

Aug 23, 1999

Appl. No.:

09/378874

Inventors:

Frederick F. Becker - Houston TX
Peter R. C. Gascoyne - Belaire TX
Ying Huang - San Diego CA
Xiao-Bo Wang - San Diego CA
Jun Yang - Houston TX

Assignee:

Board of Regents, The University of Texas System - Austin TX

International Classification:

G01N 2726

US Classification:

204547, 204643

Abstract:

Methods and apparatus for discriminating matter in a chamber having an inlet port and an outlet port utilizing dielectrophoresis and field flow fractionation. A carrier medium is introduced into the inlet port and is directed from the inlet port to the outlet port according to a velocity profile. A programmed voltage signal is applied to an electrode element coupled to the chamber to form a dielectrophoretic force on the matter. The dielectrophoretic force is balanced with a gravitational force to displace the matter to positions within said velocity profile in the carrier medium to discriminate the matter. A chamber having a top and bottom outlet port may be utilized to withdraw a first portion of a carrier medium from the top outlet port at a first, controllable fluid flow rate and to withdraw a second portion of the carrier medium from the bottom outlet port at a second, controllable fluid flow rate.

US Patent:

6887362, May 3, 2005

Filed:

Feb 6, 2002

Appl. No.:

10/072660

Inventors:

Ying Huang - San Diego CA, US
Karla Ewalt - San Diego CA, US
Robert Haigis - San Diego CA, US
Anita Forster - Santee CA, US
Michael K. Krihak - San Diego CA, US

Assignee:

Nanogen, Inc. - San Diego CA

International Classification:

G01N027/26

US Classification:

204547, 204643, 4351737, 4351739, 435 6, 435 72, 435 71, 4352852, 4352872, 4353061, 422 681, 422 50

Abstract:

This invention relates to devices and methods for performing active, multi-step molecular and biological sample preparation and diagnostic analyses employing immunochemical techniques. It relates generally to bioparticle separation, bioparticle enrichment, and electric field-mediated immunochemical detection on active electronic matrix devices utilizing AC and DC electric fields. More specifically, the invention relates to devices and methods for sample preparation/manipulation, immunoimmobilization, and immunoassays, all of which can be conducted on one or more active electronic chip devices within a single system. These manipulations are useful in a variety of applications, including, for example, detection of pathogenic bacteria and biological warfare agents, point-of-care diagnostics, food or medical product quality control assays, and other biological assays.

US Patent:

7686934, Mar 30, 2010

Filed:

Dec 12, 2006

Appl. No.:

11/638093

Inventors:

Dalibor Hodko - Poway CA, US
Ying Huang - San Diego CA, US
Daniel D. Smolko - Jamul CA, US

Assignee:

Gamida for Life B.V. - Rotterdam

International Classification:

G01N 27/447
G01N 27/453

US Classification:

204547, 204643

Abstract:

Devices and methods for performing separation of cell particles by dielectrophoresis in three dimensions. In one embodiment the device comprises a flow chamber having microfabricated chip with a plurality of vertical and horizontal electrodes, which may be coated with a protective layer to prevent direct contact between any electrode and a sample. The horizontal electrodes are in electrical communication with a DC or an AC power source, while the vertical electrodes are in electrical communication with an AC power source.

US Patent:

7855748, Dec 21, 2010

Filed:

Dec 3, 2007

Appl. No.:

11/949542

Inventors:

Giuseppe Rossi - Los Angeles CA, US
Laurent Blanquart - Westlake Village CA, US
Ying Huang - Newbury Park CA, US

Assignee:

AltaSens, Inc. - Westlake Village CA

International Classification:

H04N 5/225

US Classification:

348372, 348241, 327539

Abstract:

The claimed subject matter provides systems and/or methods that facilitate generating and/or maintaining low noise reference voltages for CMOS imaging System-on-Chip (iSoC) sensors. A primary reference voltage can be generated utilizing a low noise bandgap. Further, the primary reference voltage can be filtered via a low pass filter. The filtered, primary reference voltage can thereafter be distributed to a plurality of isolated domains. Each of the isolated domains can generate an independent set of reference voltages based upon the filtered, primary reference voltage. Moreover, subsets of these reference voltages can be employed by programmable digital to analog converters (DACs). Each of the reference voltages can be isolated from switching noise and/or clock glitches generated within each domain. Further, each DAC output can be buffered to have adequately low impedance with appropriate drive capability and requisite signal swing.

US Patent:

20040011651, Jan 22, 2004

Filed:

May 13, 2003

Appl. No.:

10/436647

Inventors:

Frederick Becker - Houston TX, US
Peter Gascoyne - Belaire TX, US
Ying Huang - San Diego CA, US
Xiaobo Wang - San Diego CA, US
Jun Yang - Houston TX, US

Assignee:

Board of Regents, The University of Texas System

International Classification:

G01N027/27

US Classification:

204/547000, 204/643000

Abstract:

Methods and apparatus for discriminating matter in a chamber having an inlet port and an outlet port utilizing dielectrophoresis and field flow fractionation. A carrier medium is introduced into the inlet port and is directed from the inlet port to the outlet port according to a velocity profile. A programmed voltage signal is applied to an electrode element coupled to the chamber to form a dielectrophoretic force on the matter. The dielectrophoretic force is balanced with a gravitational force to displace the matter to positions within said velocity profile in the carrier medium to discriminate the matter. A chamber having a top and bottom outlet port may be utilized to withdraw a first portion of a carrier medium from the top outlet port at a first, controllable fluid flow rate and to withdraw a second portion of the carrier medium from the bottom outlet port at a second, controllable fluid flow rate.

US Patent:

20100167294, Jul 1, 2010

Filed:

Sep 23, 2009

Appl. No.:

12/565206

Inventors:

Ying Huang - San Diego CA, US
James Light, II - San Diego CA, US
Elizabeth Mather - San Diego CA, US
William Weisburg - San Diego CA, US

International Classification:

C12Q 1/68
C12M 1/00

US Classification:

435 6, 4352877

Abstract:

Systems and methods are provided for immobilizing nucleic acid amplicons and protein antigens on a test device. Amplicons comprising a synthetic binding unit and a detectable label are generated and immobilized at predetermined locations on a test device by specific binding interactions between the synthetic binding unit and a synthetic capture unit located at the predetermined locations. The synthetic binding unit may include a unique design such that during amplification, a region of the synthetic binding unit is not subject to the amplification reaction, and thus the amplicon remains single stranded and available for binding to the synthetic capture unit during the capture process. In certain embodiments, the synthetic binding unit and a synthetic capture unit include synthetic nucleic acid analogs that do not interact with native nucleic acids or enzymes that act thereon. In one embodiment the synthetic binding unit and synthetic capture unit comprises puranosyl RNA (pRNA).

US Patent:

20220273617, Sep 1, 2022

Filed:

Feb 21, 2022

Appl. No.:

17/676684

Inventors:

Ying Huang - Arcadia CA, US
Bradley Tram Andresen - Upland CA, US
Jinghua Jeffrey Wang - Aradia CA, US
Vijay Kumar Nekkanti - Manchester MO, US
Ayaz Shahid - Pomona CA, US

International Classification:

A61K 31/403
A61P 9/10
A61P 9/12
A61P 9/04
A61P 35/00

Abstract:

A novel prophylactic therapy for cancer, as well as therapy against DNA damage, inflammation and immunosuppression involves the β-blocker carvedilol, which is a racemic mixture consisting of two enantiomers, S- and R-carvedilol, in 1:1 ratio. S-carvedilol is a β-blocker, with a highly potent antagonizing activity against the β-adrenergic receptors, which is the main mechanism for the drug's pharmacological activity in treatment of high blood pressure and heart failure. Carvedilol—the racemic mixture—prevents ultraviolet radiation induced skin cancer by attenuating DNA damage, reducing inflammation and reversing immunosuppression. The non-β-blocking enantiomer R-carvedilol exhibits the same cancer preventive efficacy as the racemic carvedilol, without disturbing the cardiovascular system. Both carvedilol and R-carvedilol prevent chemical carcinogen-induced lung cancer development and lung inflammation.

US Patent:

20160199345, Jul 14, 2016

Filed:

Sep 12, 2014

Appl. No.:

14/915965

Inventors:

- Pomona CA, US
Ying HUANG - Arcadia CA, US
Jinghua Jeffrey WANG - Arcadia CA, US
Ranadheer RAVULA - Atlanta GA, US
Zhi Jun WANG - Chino Hills CA, US

International Classification:

A61K 31/365
A61K 31/517
A61K 31/337
A61K 31/704

Abstract:

The invention provides compositions comprising wilforlide A for overcoming drug resistance in cancer therapy, particularly in the treatment of cancers which have exhibited resistance to treatment by chemotherapeutic drugs. Methods and compositions for treating a cancer are disclosed. The method administers to a patient in need thereof a combination of wilforlide A and a chemotherapeutic drug. Wilforlide A and chemotherapeutic drug are administered in a combined amount effective to treat the can cer. The cancer being treated is at least in part resistant to treatment by the chemotherapeutic drug alone.