Ying Ci Huang

US Patent:

6641708, Nov 4, 2003

Filed:

Aug 23, 1999

Appl. No.:

09/378874

Inventors:

Frederick F. Becker - Houston TX
Peter R. C. Gascoyne - Belaire TX
Ying Huang - San Diego CA
Xiao-Bo Wang - San Diego CA
Jun Yang - Houston TX

Assignee:

Board of Regents, The University of Texas System - Austin TX

International Classification:

G01N 2726

US Classification:

204547, 204643

Abstract:

Methods and apparatus for discriminating matter in a chamber having an inlet port and an outlet port utilizing dielectrophoresis and field flow fractionation. A carrier medium is introduced into the inlet port and is directed from the inlet port to the outlet port according to a velocity profile. A programmed voltage signal is applied to an electrode element coupled to the chamber to form a dielectrophoretic force on the matter. The dielectrophoretic force is balanced with a gravitational force to displace the matter to positions within said velocity profile in the carrier medium to discriminate the matter. A chamber having a top and bottom outlet port may be utilized to withdraw a first portion of a carrier medium from the top outlet port at a first, controllable fluid flow rate and to withdraw a second portion of the carrier medium from the bottom outlet port at a second, controllable fluid flow rate.

US Patent:

6887362, May 3, 2005

Filed:

Feb 6, 2002

Appl. No.:

10/072660

Inventors:

Ying Huang - San Diego CA, US
Karla Ewalt - San Diego CA, US
Robert Haigis - San Diego CA, US
Anita Forster - Santee CA, US
Michael K. Krihak - San Diego CA, US

Assignee:

Nanogen, Inc. - San Diego CA

International Classification:

G01N027/26

US Classification:

204547, 204643, 4351737, 4351739, 435 6, 435 72, 435 71, 4352852, 4352872, 4353061, 422 681, 422 50

Abstract:

This invention relates to devices and methods for performing active, multi-step molecular and biological sample preparation and diagnostic analyses employing immunochemical techniques. It relates generally to bioparticle separation, bioparticle enrichment, and electric field-mediated immunochemical detection on active electronic matrix devices utilizing AC and DC electric fields. More specifically, the invention relates to devices and methods for sample preparation/manipulation, immunoimmobilization, and immunoassays, all of which can be conducted on one or more active electronic chip devices within a single system. These manipulations are useful in a variety of applications, including, for example, detection of pathogenic bacteria and biological warfare agents, point-of-care diagnostics, food or medical product quality control assays, and other biological assays.

US Patent:

7686934, Mar 30, 2010

Filed:

Dec 12, 2006

Appl. No.:

11/638093

Inventors:

Dalibor Hodko - Poway CA, US
Ying Huang - San Diego CA, US
Daniel D. Smolko - Jamul CA, US

Assignee:

Gamida for Life B.V. - Rotterdam

International Classification:

G01N 27/447
G01N 27/453

US Classification:

204547, 204643

Abstract:

Devices and methods for performing separation of cell particles by dielectrophoresis in three dimensions. In one embodiment the device comprises a flow chamber having microfabricated chip with a plurality of vertical and horizontal electrodes, which may be coated with a protective layer to prevent direct contact between any electrode and a sample. The horizontal electrodes are in electrical communication with a DC or an AC power source, while the vertical electrodes are in electrical communication with an AC power source.

US Patent:

20040011651, Jan 22, 2004

Filed:

May 13, 2003

Appl. No.:

10/436647

Inventors:

Frederick Becker - Houston TX, US
Peter Gascoyne - Belaire TX, US
Ying Huang - San Diego CA, US
Xiaobo Wang - San Diego CA, US
Jun Yang - Houston TX, US

Assignee:

Board of Regents, The University of Texas System

International Classification:

G01N027/27

US Classification:

204/547000, 204/643000

Abstract:

Methods and apparatus for discriminating matter in a chamber having an inlet port and an outlet port utilizing dielectrophoresis and field flow fractionation. A carrier medium is introduced into the inlet port and is directed from the inlet port to the outlet port according to a velocity profile. A programmed voltage signal is applied to an electrode element coupled to the chamber to form a dielectrophoretic force on the matter. The dielectrophoretic force is balanced with a gravitational force to displace the matter to positions within said velocity profile in the carrier medium to discriminate the matter. A chamber having a top and bottom outlet port may be utilized to withdraw a first portion of a carrier medium from the top outlet port at a first, controllable fluid flow rate and to withdraw a second portion of the carrier medium from the bottom outlet port at a second, controllable fluid flow rate.

US Patent:

20100167294, Jul 1, 2010

Filed:

Sep 23, 2009

Appl. No.:

12/565206

Inventors:

Ying Huang - San Diego CA, US
James Light, II - San Diego CA, US
Elizabeth Mather - San Diego CA, US
William Weisburg - San Diego CA, US

International Classification:

C12Q 1/68
C12M 1/00

US Classification:

435 6, 4352877

Abstract:

Systems and methods are provided for immobilizing nucleic acid amplicons and protein antigens on a test device. Amplicons comprising a synthetic binding unit and a detectable label are generated and immobilized at predetermined locations on a test device by specific binding interactions between the synthetic binding unit and a synthetic capture unit located at the predetermined locations. The synthetic binding unit may include a unique design such that during amplification, a region of the synthetic binding unit is not subject to the amplification reaction, and thus the amplicon remains single stranded and available for binding to the synthetic capture unit during the capture process. In certain embodiments, the synthetic binding unit and a synthetic capture unit include synthetic nucleic acid analogs that do not interact with native nucleic acids or enzymes that act thereon. In one embodiment the synthetic binding unit and synthetic capture unit comprises puranosyl RNA (pRNA).