Ying Jiang

US Patent:

8450054, May 28, 2013

Filed:

Dec 9, 2010

Appl. No.:

12/928337

Inventors:

Ying Jiang - Eugene OR, US
Daniel J. Coleman - Corvallis OR, US
John J. Naleway - Eugene OR, US
Gabriele M. Cook - Eugene OR, US

Assignee:

Marker Gene Technologies, Inc. - Eugene OR

International Classification:

C12Q 1/00
C12Q 1/66

US Classification:

435 4, 435 8

Abstract:

A codon optimized and stabilized luciferase gene based upon the sequence of the natural luciferase gene isolated from (Japanese firefly) and a novel recombinant DNA characterized by incorporating this new gene coding for a novel luciferase into a vector DNA for improved activities in mammalian cells, are disclosed. This new luciferase exhibits long-wavelength light emission, as well as improved thermostability and higher expression levels in mammalian cell systems, compared to native luciferase. Assays using this new enzyme for measuring various biological metabolic functions are described.

US Patent:

20150152476, Jun 4, 2015

Filed:

Dec 3, 2014

Appl. No.:

14/544153

Inventors:

- EUGENE OR, US
YING JIANG - EUGENE OR, US
RYAN LINK-COLE - MONMOUTH OR, US

International Classification:

C12Q 1/68
C07D 271/12
C07D 209/24
C07J 19/00
C07D 495/04
C09B 23/08
C09B 26/02
C07D 311/16
C07D 471/04
C09B 23/06
C09B 11/24
C07C 233/31

Abstract:

The present invention provides systems and methods for production of activatable diazo-derivatives for use in labeling nucleotides. Labeling nucleotides is accomplished by contacting a stable hydrazide derivative of a detectable moiety with an activating polymer reagent which is used to directly label the nucleotide sample. Labeling occurs on the phosphate backbone of the nucleotide which does not perturb hybridization of the labeled nucleotide with its anti-sense strand. Since the method involves direct labeling, all types of nucleotides can be labeled without prior amplification or alteration.

US Patent:

20140087402, Mar 27, 2014

Filed:

Dec 4, 2012

Appl. No.:

13/694453

Inventors:

- Eugene OR, US
John J. Naleway - Eugene OR, US
Gabriele M. Cook - Eugene OR, US
Ying Jiang - Eugene OR, US

Assignee:

Marker Gene Technologies, Inc. - Eugene OR

International Classification:

C12N 9/02
C12Q 1/66

US Classification:

435 8, 536 232, 4353201, 435189

Abstract:

A codon optimized and stabilized luciferase gene and a novel recombinant DNA characterized by incorporating this new gene coding for a novel luciferase into a vector DNA for improved activities in mammalian cells, are disclosed. This new luciferase exhibits long-wavelength light emission, as well as improved thermostability and higher expression levels in mammalian cell systems, compared to native luciferase. Assays using this new enzyme for measuring various biological metabolic functions are described.