Yong Huang

US Patent:

20220410470, Dec 29, 2022

Filed:

Jul 7, 2022

Appl. No.:

17/859593

Inventors:

- Gainesville FL, US
Yong HUANG - Gainesville FL, US
Kaidong SONG - Gainesville FL, US
Wenxuan CHAI - Gainesville FL, US

International Classification:

B29C 64/118
B33Y 70/10
B29C 64/40
A61L 27/44
A61L 27/56
A61L 27/52

Abstract:

Described herein are apparatuses, systems, and methods for fabricating tissue constructs, such as by fabricating perfusable tissue constructs by embedding a sacrificial material into a composite matrix yield stress support bath. A composite matrix bath can include a microgel filler and a hydrogel precursor. An extrusion tip can be used for embedded printing of perfusable tissue constructs by disposing sacrificial material into the composite matrix bath while the extrusion tip travels along a predefined course through the composite matrix bath. This sacrificial material can be the printed tissue construct or can be removed to render the matrix bath a perfusable tissue construct. The composite matrix bath can include acellular or cell-laden hydrogels. The sacrificial material can include a salt and a physiological buffer or a non-cytotoxic porogen material. The hydrogel precursor can include at least one of gellan and gelatin. Cross-linking can be carried out chemically, thermally, enzymatically, or physically.

US Patent:

20200189182, Jun 18, 2020

Filed:

Dec 4, 2019

Appl. No.:

16/703691

Inventors:

- Gainesville FL, US
Yong Huang - Gainesville FL, US
Kaidong Song - Gainesville FL, US
Wenxuan Chai - Gainesville FL, US

International Classification:

B29C 64/118
B33Y 10/00
B33Y 30/00
B33Y 70/10
B29C 64/40
A61L 27/44
A61L 27/52
A61L 27/56

Abstract:

Described herein are apparatuses, systems, and methods for fabricating tissue constructs, such as by fabricating perfusable tissue constructs by embedding a sacrificial material into a composite matrix yield stress support bath. A composite matrix bath can include a microgel filler and a hydrogel precursor. An extrusion tip can be used for embedded printing of perfusable tissue constructs by disposing sacrificial material into the composite matrix bath while the extrusion tip travels along a predefined course through the composite matrix bath. This sacrificial material can be the printed tissue construct or can be removed to render the matrix bath a perfusable tissue construct. The composite matrix bath can include acellular or cell-laden hydrogels. The sacrificial material can include a salt and a physiological buffer or a non-cytotoxic porogen material. The hydrogel precursor can include at least one of gellan and gelatin. Cross-linking can be carried out chemically, thermally, enzymatically, or physically.